##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926898_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 683934 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.80895525006799 31.0 28.0 31.0 16.0 34.0 2 27.801062675638292 31.0 28.0 31.0 16.0 34.0 3 27.745671365950514 31.0 28.0 31.0 15.0 34.0 4 31.361779060552628 35.0 32.0 37.0 17.0 37.0 5 31.109121640392203 35.0 32.0 37.0 16.0 37.0 6 30.553288767629624 35.0 30.0 37.0 11.0 37.0 7 30.677634099196705 35.0 31.0 37.0 11.0 37.0 8 30.400094161132508 35.0 30.0 37.0 10.0 37.0 9 32.63994625212374 37.0 32.0 39.0 16.0 39.0 10 32.83001137536663 37.0 33.0 39.0 15.0 39.0 11 32.99978799123892 37.0 33.0 39.0 16.0 39.0 12 32.86133018683089 37.0 33.0 39.0 11.0 39.0 13 32.97277807507742 38.0 33.0 39.0 11.0 39.0 14 33.96795743448929 39.0 33.0 41.0 11.0 41.0 15 33.512325750730334 39.0 33.0 40.0 10.0 41.0 16 33.600613509490685 39.0 33.0 40.0 10.0 41.0 17 33.83160217213942 39.0 33.0 41.0 10.0 41.0 18 33.88564832279138 39.0 34.0 41.0 10.0 41.0 19 33.25056803726675 39.0 32.0 40.0 8.0 41.0 20 33.518738650220634 39.0 33.0 41.0 2.0 41.0 21 33.51342819628795 39.0 33.0 41.0 2.0 41.0 22 33.53041082911509 39.0 33.0 41.0 2.0 41.0 23 33.52053853149573 39.0 34.0 41.0 2.0 41.0 24 32.22167051206696 38.0 31.0 40.0 2.0 41.0 25 32.784525992274105 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2123704969752609 1101 2 -0.28955609628594914 1101 3 -1.145053422243663 1101 4 -0.4800233994191032 1101 5 -0.6759436384727486 1101 6 -2.5685455127711876 1101 7 -0.983204646123621 1101 8 -0.49530089600764526 1101 9 -0.4709398830883771 1101 10 -9.562822950924438 1101 11 -4.39451200131732 1101 12 -1.743668021380337 1101 13 -1.0252931573604904 1101 14 -0.751157819874372 1101 15 -2.445757377982769 1101 16 -0.6461146663881934 1101 17 -0.4711934709867691 1101 18 -0.32354400296853925 1101 19 -1.1354695181695504 1101 20 -0.3971573044370871 1101 21 -0.3369507290903755 1101 22 -0.22969799849831674 1101 23 -0.2795374311248011 1101 24 -0.4002927352997574 1101 25 -0.21963797174674227 1102 1 -0.0833865821933415 1102 2 -0.4590704875199627 1102 3 -0.4277351058501715 1102 4 -0.2199617445034967 1102 5 -0.2612532865513728 1102 6 -1.454953961325959 1102 7 -0.669017911087149 1102 8 -0.05971196947466595 1102 9 -0.08765996366707896 1102 10 -0.10175406223903138 1102 11 -0.1894941458095829 1102 12 -0.1496412228576105 1102 13 -0.4413844798889528 1102 14 -0.5829345191998314 1102 15 0.1942542204667035 1102 16 -0.10499205856839922 1102 17 -0.2934899639819193 1102 18 -0.5118143078856718 1102 19 -0.03920849201199417 1102 20 -0.11960335638593023 1102 21 -0.30872376574757965 1102 22 -0.25996689939261586 1102 23 -0.3909374860648285 1102 24 -3.7939624236677325 1102 25 -2.05417388115675 1103 1 0.05271860615065194 1103 2 -0.11811709123607628 1103 3 0.28026062975750676 1103 4 0.13663283362626544 1103 5 -0.40740518014463234 1103 6 0.22848090515794794 1103 7 0.1347286621690209 1103 8 0.3415673482225614 1103 9 0.20114600648217618 1103 10 0.3479007250490227 1103 11 0.20224105573372242 1103 12 -0.1368277392552173 1103 13 -0.0905366780816621 1103 14 -0.010790139059714932 1103 15 0.3223992098057238 1103 16 0.2794581394212443 1103 17 0.011693303354867624 1103 18 -0.20020399211757223 1103 19 -1.7142871802036872 1103 20 -0.549504869229871 1103 21 -0.4394172371659977 1103 22 -0.23645689837728412 1103 23 -0.34230818359757365 1103 24 1.1428941099905074 1103 25 0.5128481515369288 1104 1 -0.30242171256320916 1104 2 -0.23064302954822224 1104 3 -1.2140208538132171 1104 4 -0.4369292269334011 1104 5 -0.38331327289802175 1104 6 -1.7279699278899798 1104 7 -0.3503751918108726 1104 8 0.08642702950869818 1104 9 0.20208381497180739 1104 10 0.31319979630634975 1104 11 0.27805949762182536 1104 12 0.3291955901160222 1104 13 0.10705987287830965 1104 14 -0.18323508939918298 1104 15 0.2792036097493096 1104 16 -0.11297190268444268 1104 17 -0.04226140784847132 1104 18 -0.20776790613332707 1104 19 -1.396301604927178 1104 20 -0.3431205188460851 1104 21 -0.16054462197974573 1104 22 -0.07956486219460146 1104 23 -0.029802391547285367 1104 24 1.277549709643992 1104 25 0.651672012769474 1105 1 -0.295783136009625 1105 2 -0.271272890141006 1105 3 -0.017265502646676367 1105 4 -0.20627408523884228 1105 5 -0.34478198452492137 1105 6 -0.7860885323731637 1105 7 -0.6656396802899138 1105 8 -0.03571488174259585 1105 9 -1.1200735057129485 1105 10 -0.6384656263695945 1105 11 -0.6918555097217265 1105 12 -0.5992468532422777 1105 13 -0.5809597741493562 1105 14 -0.7505064158143213 1105 15 -0.26415131284236537 1105 16 -0.7666593319575483 1105 17 -0.6488691655351246 1105 18 -0.601085027458538 1105 19 -1.2229531831902491 1105 20 -0.5016103943667574 1105 21 -0.6577348080968051 1105 22 -0.6625345489497079 1105 23 -0.8357427281470962 1105 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8 1.2533800290960144 1110 9 1.031624827844972 1110 10 1.0481318672296496 1110 11 0.6765485687941961 1110 12 0.7553998700205398 1110 13 0.5956210999466762 1110 14 0.49401498741076466 1110 15 0.7953536954673908 1110 16 0.6350205853941091 1110 17 0.3827366378157251 1110 18 0.5854370046126078 1110 19 -1.7294204158183746 1110 20 0.19655068006166232 1110 21 0.36606252379916526 1110 22 0.5486804714877849 1110 23 0.5907864907362494 1110 24 -0.33542362594364405 1110 25 0.008875512757654747 1111 1 -0.00321181029636719 1111 2 -0.3831521089331922 1111 3 0.24038187382708998 1111 4 0.06713964889849677 1111 5 -0.006305699762073402 1111 6 0.18473137582591903 1111 7 0.021828594392832912 1111 8 0.48619043731059364 1111 9 0.025500250024613536 1111 10 -0.04069404865381898 1111 11 -0.35175020866871876 1111 12 -0.5100109608833492 1111 13 -0.5564264288159038 1111 14 -0.4866768775189385 1111 15 -1.4291031623608603 1111 16 -0.8248494138583666 1111 17 -0.6537220140636109 1111 18 -0.41276894429771005 1111 19 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0.9799348406094346 2215 6 1.4091450352167492 2215 7 0.9625909935422463 2215 8 -0.023929019816179675 2215 9 0.41216295563472016 2215 10 0.11974437776434144 2215 11 0.06787578420137663 2215 12 -0.08684503430882984 2215 13 0.022330841807651325 2215 14 0.16968374540932984 2215 15 0.22469945193647334 2215 16 0.4769330615935061 2215 17 -0.08972489104153425 2215 18 -0.6564302114188578 2215 19 0.342181169848196 2215 20 -3.213565747818599 2215 21 -1.8336752793659272 2215 22 -0.9618979222444608 2215 23 -0.6761891936287014 2215 24 -0.2192470176469854 2215 25 -0.21075959664709387 2216 1 -0.259456718524671 2216 2 -1.0255150504197488 2216 3 -0.38646014946327156 2216 4 -0.24622894744980428 2216 5 -0.31287827847487293 2216 6 -1.6311202080145932 2216 7 -9.369584881430239 2216 8 -10.851127456972243 2216 9 -5.793334512998346 2216 10 -3.3179619836708305 2216 11 -2.306942617735668 2216 12 -1.842416793058554 2216 13 -1.66426116787758 2216 14 -1.7640137012118515 2216 15 -2.7689968940903817 2216 16 -1.9125177418403503 2216 17 -2.295797420207286 2216 18 -2.0720036210600625 2216 19 -1.265864174637823 2216 20 -2.215483533422823 2216 21 -4.867779945885847 2216 22 -3.2737017963364714 2216 23 -3.223894454469555 2216 24 -3.614550231382406 2216 25 -2.8241648354760613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45361.0 3 1.0 4 403.0 5 2183.0 6 2580.0 7 2056.0 8 1780.0 9 1537.0 10 1424.0 11 1455.0 12 1408.0 13 1305.0 14 1387.0 15 1349.0 16 1480.0 17 1608.0 18 1891.0 19 2294.0 20 2977.0 21 3761.0 22 4703.0 23 5668.0 24 6447.0 25 7510.0 26 8528.0 27 9330.0 28 10399.0 29 12338.0 30 14372.0 31 17578.0 32 22579.0 33 29398.0 34 39579.0 35 54768.0 36 78691.0 37 125801.0 38 158005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.505649522966614 18.15818664306532 12.895799966883875 25.44036386708418 2 15.077105071673385 19.387024562646683 40.941746250891256 24.594124114788684 3 19.06221129674339 23.9138169077804 29.487519234682896 27.53645256079331 4 12.163457247493259 16.14088377176618 38.129831137877 33.56582784286356 5 13.89240279559105 37.44375108768615 35.16556959655801 13.498276520164787 6 32.4070336767106 36.24516668714061 17.844942058742667 13.502857577406127 7 28.24244860002259 31.02480194672326 22.349327696949516 18.383421756304628 8 25.391117127608243 36.07532093824111 20.986933673985707 17.54662826016494 9 25.984296876485235 15.942808925832061 19.8596692370575 38.213224960625205 10 14.998135160632145 29.09558947206763 33.69786677002509 22.208408597275138 11 34.57535560686778 22.98184785422129 23.321697904891707 19.121098634019223 12 23.07263721752559 24.805249311590867 30.888694881570235 21.233418589313317 13 28.694599289888217 21.714563140870034 25.55539632309501 24.03544124614674 14 21.308521917157165 21.53401820641246 27.16104266442438 29.996417212005994 15 23.738551730894937 29.60062120066624 23.03195059641406 23.628876472024764 16 22.812590028531275 28.09884602616805 26.466816123168535 22.62174782213214 17 22.282065868307257 28.336591992910403 26.90434164596187 22.47700049282047 18 22.312886970305833 27.865225389639065 27.89096247941764 21.93092516063746 19 24.07142000567429 26.948050998411784 26.837197602758756 22.14333139315517 20 23.56904178700691 27.173326508975247 27.340328300369247 21.917303403648596 21 23.555252040319434 26.54703811862149 26.860562208356058 23.03714763270302 22 23.288931264778956 26.972026550574924 27.02584133121389 22.713200853432227 23 22.970012664114346 26.97529474044415 26.926451620450937 23.128240974990568 24 23.492431631699553 26.87819979439425 27.064648825078635 22.564719748827564 25 23.197385353299016 26.81562938699111 27.09493448759944 22.892050772110434 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 271.5 3 359.0 4 359.0 5 359.0 6 529.5 7 700.0 8 700.0 9 700.0 10 1033.0 11 1366.0 12 1366.0 13 1366.0 14 2208.0 15 3050.0 16 3050.0 17 3050.0 18 5176.5 19 7303.0 20 7303.0 21 7303.0 22 11263.5 23 15224.0 24 15224.0 25 15224.0 26 21538.0 27 27852.0 28 27852.0 29 27852.0 30 35860.5 31 43869.0 32 43869.0 33 43869.0 34 51344.0 35 58819.0 36 58819.0 37 58819.0 38 65573.5 39 72328.0 40 72328.0 41 72328.0 42 77794.5 43 83261.0 44 83261.0 45 83261.0 46 87137.5 47 91014.0 48 91014.0 49 91014.0 50 89227.5 51 87441.0 52 87441.0 53 87441.0 54 79509.5 55 71578.0 56 71578.0 57 71578.0 58 62121.0 59 52664.0 60 52664.0 61 52664.0 62 43281.0 63 33898.0 64 33898.0 65 33898.0 66 26217.5 67 18537.0 68 18537.0 69 18537.0 70 13727.5 71 8918.0 72 8918.0 73 8918.0 74 6272.0 75 3626.0 76 3626.0 77 3626.0 78 2467.5 79 1309.0 80 1309.0 81 1309.0 82 885.0 83 461.0 84 461.0 85 461.0 86 292.0 87 123.0 88 123.0 89 123.0 90 76.0 91 29.0 92 29.0 93 29.0 94 21.0 95 13.0 96 13.0 97 13.0 98 10.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21741863981027407 2 0.1323227095012092 3 0.13495454239736582 4 0.06974357174815114 5 0.020908450230577802 6 0.02017738553720096 7 0.3177207157415774 8 0.30777823591165226 9 0.01769176557971968 10 0.03348276295665956 11 0.015059932683563034 12 0.01622963619296599 13 0.014328867990186187 14 0.01593721031561525 15 0.014182655051510817 16 0.01798419145707042 17 0.016814487947667465 18 0.014036442112835449 19 0.02163951492395465 20 0.01593721031561525 21 0.01389022917416008 22 0.015644784438264513 23 0.016375849131641355 24 0.014767506806212296 25 0.013744016235484711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 683934.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.38629798270405 #Duplication Level Percentage of deduplicated Percentage of total 1 92.99075164613195 81.26117532984513 2 5.209826359934143 9.105348774547027 3 0.946501761540257 2.4813385492533357 4 0.3391389403842072 1.1854438600781134 5 0.16367037721128497 0.715127417696346 6 0.09208428269229645 0.48281427401235444 7 0.05475454426840991 0.33493578449425 8 0.041468694488612075 0.28990365548284575 9 0.02943450431819538 0.23149551287907094 >10 0.11681291143193052 1.7348002571917567 >50 0.008363538075117234 0.5281152629847401 >100 0.006356109347354967 1.1365992271763248 >500 6.690641410506995E-4 0.35767210740831945 >1k 1.6726603526267487E-4 0.15522998695040263 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1062 0.15527814087324215 No Hit GTACATGGGAAGCAGTGGTATCAAC 710 0.10381118645951218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.4621293867536926E-4 5 0.0 0.0 0.0 0.0 1.4621293867536926E-4 6 0.0 0.0 0.0 0.0 1.4621293867536926E-4 7 0.0 0.0 0.0 0.0 1.4621293867536926E-4 8 0.0 0.0 0.0 0.0 1.4621293867536926E-4 9 0.0 0.0 0.0 0.0 1.4621293867536926E-4 10 0.0 0.0 0.0 0.0 1.4621293867536926E-4 11 0.0 0.0 0.0 1.4621293867536926E-4 1.4621293867536926E-4 12 0.0 0.0 0.0 1.4621293867536926E-4 1.4621293867536926E-4 13 0.0 0.0 0.0 1.4621293867536926E-4 1.4621293867536926E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 85 1.6392733E-8 14.576628 3 GGATAAC 40 0.005137605 14.306822 1 CTTCGTG 55 1.9842909E-4 13.796158 9 CCGTGCA 55 1.9842909E-4 13.796158 9 TAGGACT 70 7.078841E-6 13.602532 4 GTCCTAC 290 0.0 12.169021 1 TAGGTAG 55 0.0030182695 12.115951 5 CTGCGTT 135 3.8198777E-10 11.940402 11 TGCGTTG 135 3.8198777E-10 11.940402 12 TTAGGAC 145 9.094947E-11 11.831427 3 TTCTATA 105 2.596771E-7 11.800993 2 CGTTGAT 145 1.0004442E-10 11.770863 14 GCCTTAT 65 7.750107E-4 11.738932 1 GTTATGT 65 7.750107E-4 11.738932 1 TAGGACC 115 6.8263034E-8 11.591723 4 GTCCCGG 75 1.995404E-4 11.445457 1 CCTACAG 355 0.0 11.27596 3 TCCTACA 330 0.0 11.264585 2 GACCTAC 60 0.005698553 11.127529 1 TCTGCGT 145 1.2423698E-9 11.120178 10 >>END_MODULE