FastQCFastQC Report
Thu 26 May 2016
SRR1926898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683934
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16800.24563773697462038No Hit
GGTATCAACGCAGAGTACTTTTTTT10660.15586299262794365No Hit
TATCAACGCAGAGTACTTTTTTTTT8810.12881359897300032No Hit
GTACATGGGAAGCAGTGGTATCAAC7710.11273017571870969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCAC759.393825E-713.9665923
GTAGGAC3100.013.2088733
GACGTGA1403.6379788E-1212.8763867
GTCCTAG1051.8895662E-812.7118261
TAGGACC1950.012.695974
TGGCGAG2850.012.65233418
AGGACGT2500.012.188135
CTAATGC550.003011157312.1197693
CTATCCC550.003012813212.1188794
ATGGCGA3100.011.93808917
TATGGCG2950.011.90177216
TAGGACG2650.011.8575564
TTAAGAC657.854601E-411.7202173
GTCCTAC3100.011.6866791
AAGTGTA658.09974E-411.677377
TGTAGGA3800.011.5376092
GGCGAGA1900.011.48698719
CCCTAGG752.0032236E-411.4406441
TAGGATC752.0265936E-411.4263734
TCCTACA3600.011.3843322
Produced by FastQC (version 0.11.5)