FastQCFastQC Report
Thu 26 May 2016
SRR1926897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613255
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7820.1275162860474028No Hit
GTACATGGGAAGCAGTGGTATCAAC7440.12131984247988194No Hit
CCCATGTACTCTGCGTTGATACCAC6660.10860082673602334No Hit
GTCCTACAGTGGACATTTCTAAATT6240.10175212595086873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAG456.847318E-414.75107411
TCCAACG551.9873622E-413.79321218
GTATTAC905.096299E-712.7247211
TATTAGG603.9638608E-412.7122012
CTTACAC550.002983497212.1343733
TAAGATA550.003011101212.1194684
GACGTGG1459.458745E-1111.8122527
AACAGTG1459.458745E-1111.8122527
CTCCTAT657.70961E-411.7458961
CTATACC752.025023E-411.4269264
GGCGAGA1950.011.18487919
AAATGTC600.00579829611.101337
TAGAACA2000.010.9508054
GTCCTAC3400.010.9470021
ATTAGGG700.001445230710.8961723
GAACAGT1502.062734E-910.7841486
CCCTAGA909.0623E-510.6039341
GTCCTAA1803.274181E-1110.6039341
TGGCGAG2800.010.49885218
TATGGCG3100.010.40052716
Produced by FastQC (version 0.11.5)