##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926897_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613255 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.00877122893413 31.0 28.0 31.0 16.0 34.0 2 27.99268656594728 31.0 28.0 31.0 16.0 34.0 3 27.937998059534777 31.0 28.0 33.0 16.0 34.0 4 31.584857848692632 35.0 32.0 37.0 18.0 37.0 5 31.330423722595004 35.0 32.0 37.0 17.0 37.0 6 30.785459555975898 35.0 31.0 37.0 11.0 37.0 7 30.916892646615192 35.0 32.0 37.0 11.0 37.0 8 30.657599204246196 35.0 31.0 37.0 10.0 37.0 9 32.887118735273255 37.0 33.0 39.0 17.0 39.0 10 33.06688897766834 37.0 33.0 39.0 17.0 39.0 11 33.256800189154596 38.0 33.0 39.0 17.0 39.0 12 33.10631955711735 38.0 33.0 39.0 17.0 39.0 13 33.224502042380415 38.0 33.0 39.0 17.0 39.0 14 34.2438545140276 39.0 34.0 41.0 17.0 41.0 15 33.79087002959617 39.0 33.0 41.0 10.0 41.0 16 33.87353874000212 39.0 33.0 40.0 11.0 41.0 17 34.09244441545523 39.0 34.0 41.0 17.0 41.0 18 34.14212358643631 39.0 34.0 41.0 16.0 41.0 19 33.49262215554704 39.0 33.0 40.0 10.0 41.0 20 33.78187703320805 39.0 33.0 41.0 10.0 41.0 21 33.776861175204445 39.0 34.0 41.0 8.0 41.0 22 33.79130704193199 39.0 34.0 41.0 2.0 41.0 23 33.77928268012491 39.0 34.0 41.0 2.0 41.0 24 32.45957228232954 38.0 32.0 40.0 2.0 41.0 25 33.032436751432925 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2603928044008903 1101 2 -0.26419274352697997 1101 3 -0.9414107423764513 1101 4 -0.19602416646870324 1101 5 -0.3946212497443291 1101 6 -2.311259514037605 1101 7 -0.41355759516851265 1101 8 -0.08335431437460983 1101 9 -0.06994870022318622 1101 10 -8.90464137848457 1101 11 -3.722550964579746 1101 12 -1.294421480236224 1101 13 -0.7209686047970649 1101 14 -0.2627047977601862 1101 15 -1.7836875704682669 1101 16 -0.11176203193677026 1101 17 0.08725640257275558 1101 18 0.11585034140081518 1101 19 -0.6246383347166713 1101 20 0.09060271708370493 1101 21 0.15101434740341801 1101 22 0.3379682878538759 1101 23 0.3325824033883933 1101 24 0.15016722298861396 1101 25 0.3602036792436465 1102 1 -0.44953481323080524 1102 2 -0.6792108530740215 1102 3 -0.7432345115800665 1102 4 -0.7454373591165648 1102 5 -0.8409103641898952 1102 6 -1.9396378270692267 1102 7 -0.9107371964199835 1102 8 -0.5659411023841088 1102 9 -0.23348830669716847 1102 10 -0.21565894643768502 1102 11 -0.485076533132478 1102 12 -0.17597561303312403 1102 13 -0.5166244895536138 1102 14 -0.694448246649678 1102 15 0.01704575738465053 1102 16 -0.11914245815184898 1102 17 -0.23800581109571795 1102 18 -0.3259597428847414 1102 19 -0.07773484965644428 1102 20 -0.5153852808079478 1102 21 -0.40638493788602403 1102 22 -0.4378976579770608 1102 23 -0.7208564762858742 1102 24 -4.331292856219498 1102 25 -2.436818279488829 1103 1 -0.48496507571646674 1103 2 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21 -0.49814788360956896 1108 22 -0.2158716880290541 1108 23 -0.07710745754724968 1108 24 -1.1918567698130857 1108 25 -0.5786763498441161 1109 1 0.12750711516259727 1109 2 0.37370316566913786 1109 3 -1.2724367776814347 1109 4 0.10799720076865782 1109 5 0.21040110518922361 1109 6 0.7770052343748617 1109 7 0.8103939229274104 1109 8 0.9987172809953648 1109 9 0.24606251963890458 1109 10 0.745318861587478 1109 11 0.38863246045732325 1109 12 -0.14508112837805243 1109 13 0.10554863176189855 1109 14 0.17608553794193682 1109 15 -1.5290235132139713 1109 16 -0.018871611214855477 1109 17 -0.025864602814884563 1109 18 0.10530307521096205 1109 19 -1.4874264796123882 1109 20 -0.41491440260170265 1109 21 -0.23535590346224922 1109 22 0.19699929143111916 1109 23 -0.06457917316723183 1109 24 -0.6835870079489652 1109 25 -0.4836692814845449 1110 1 0.6830542312525125 1110 2 0.9293826608058353 1110 3 1.0907635834312437 1110 4 0.6425850245005336 1110 5 0.8641300710267537 1110 6 1.4409729599292227 1110 7 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24 -2.011734240249268 2214 25 -0.957978623357782 2215 1 -0.010518504750312019 2215 2 -0.47332425202644046 2215 3 -0.16093700819219947 2215 4 0.06764171088797966 2215 5 0.36784901881512866 2215 6 0.7425836352139612 2215 7 0.43450534921529993 2215 8 -0.7449456860909187 2215 9 -0.5407814557759636 2215 10 -0.6713087155531028 2215 11 -0.6129317916509933 2215 12 -0.9010286081605443 2215 13 -0.4672243221782253 2215 14 -0.4710286484355848 2215 15 -0.6793155270728022 2215 16 -0.4083291156992459 2215 17 -0.8797640598563348 2215 18 -1.8640249003408087 2215 19 -0.27334740535973623 2215 20 -4.323130853219045 2215 21 -3.0380125148935697 2215 22 -1.8236289057733543 2215 23 -1.9172294430359464 2215 24 -1.9000144735585707 2215 25 -1.7697583089706903 2216 1 -1.1154723091778571 2216 2 -1.4652604914428125 2216 3 -0.8138196062353593 2216 4 -1.0849167658079324 2216 5 -0.8624342301204742 2216 6 -1.997785229074303 2216 7 -9.540341887898627 2216 8 -10.76559481834473 2216 9 -5.9612316674248405 2216 10 -3.842283909150545 2216 11 -2.680906425961677 2216 12 -2.5238213891027748 2216 13 -2.1749435204770826 2216 14 -2.329603858310069 2216 15 -3.191313694959142 2216 16 -2.48148590620708 2216 17 -2.747708032760734 2216 18 -2.522139810585216 2216 19 -1.8495462048331923 2216 20 -2.7852209580072405 2216 21 -5.375710051476698 2216 22 -3.9745871220365814 2216 23 -3.9090171071194213 2216 24 -4.040247822001795 2216 25 -3.435621848678629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37464.0 3 2.0 4 332.0 5 1926.0 6 2211.0 7 1666.0 8 1359.0 9 1320.0 10 1253.0 11 1175.0 12 1145.0 13 1137.0 14 1158.0 15 1256.0 16 1297.0 17 1430.0 18 1626.0 19 2100.0 20 2708.0 21 3290.0 22 4219.0 23 4926.0 24 5694.0 25 6693.0 26 7613.0 27 8307.0 28 9224.0 29 10809.0 30 12917.0 31 15862.0 32 20155.0 33 26647.0 34 35521.0 35 49653.0 36 71704.0 37 113382.0 38 144074.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92556661807294 18.105465813334728 12.440102242938112 25.528865325654216 2 14.980047285647109 19.49994121949945 41.070099012487596 24.44991248236585 3 19.26171822697421 23.894144887022346 29.367984820924136 27.476152065079308 4 11.980125318190717 16.19541805365185 38.365315579922985 33.45914104823445 5 13.810903954710113 37.295817927084066 35.59875195109579 13.29452616711003 6 32.441178101585 36.26553932170106 17.940638241655005 13.352644335058928 7 28.200020606518528 30.824898561959387 22.510495423226818 18.46458540829527 8 25.162597570807826 36.381461996241896 21.146106407579 17.30983402537127 9 25.929586446361323 15.642527346534042 19.872983565169314 38.55490264193532 10 14.781810114771535 29.23003895315834 34.20635386693288 21.781797065137248 11 34.868542296257274 22.614378232215575 23.505495276133754 19.011584195393393 12 23.0634868083066 24.639444443538377 31.386722030951592 20.910346717203428 13 28.867223557731513 21.615843018040792 25.397526933978725 24.11940649024897 14 20.993283949650824 21.71512073560159 27.222836528268406 30.068758786479176 15 23.844440167915376 29.99236748286761 22.898693012985063 23.26449933623195 16 22.70521788626107 27.912339902241083 27.033749170256105 22.348693041241738 17 22.230231901974566 28.251626445208082 27.256758982756175 22.261382670061174 18 22.14011729172239 27.72419484255641 28.459877489513545 21.675810376207654 19 23.943312798983555 26.78452306974666 26.914840349100256 22.35732378216953 20 23.559218927615603 27.250443198071622 27.452022891363875 21.738314982948904 21 23.439790857688962 26.657490324884904 26.787307292745417 23.115411524680717 22 23.156950526375873 27.35279007754972 26.94914010323654 22.541119292837863 23 22.853839090732656 27.223710884558994 26.95738257824719 22.96506744646116 24 23.504286746394552 26.941185872084205 27.27828706278367 22.276240318737575 25 23.036627868101185 26.858751367898144 27.238582637356544 22.866038126644128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 205.0 3 257.0 4 257.0 5 257.0 6 392.0 7 527.0 8 527.0 9 527.0 10 811.5 11 1096.0 12 1096.0 13 1096.0 14 1765.5 15 2435.0 16 2435.0 17 2435.0 18 4413.5 19 6392.0 20 6392.0 21 6392.0 22 9981.5 23 13571.0 24 13571.0 25 13571.0 26 19597.0 27 25623.0 28 25623.0 29 25623.0 30 33625.5 31 41628.0 32 41628.0 33 41628.0 34 47812.5 35 53997.0 36 53997.0 37 53997.0 38 59601.5 39 65206.0 40 65206.0 41 65206.0 42 69849.0 43 74492.0 44 74492.0 45 74492.0 46 78351.5 47 82211.0 48 82211.0 49 82211.0 50 80197.5 51 78184.0 52 78184.0 53 78184.0 54 70420.0 55 62656.0 56 62656.0 57 62656.0 58 54413.0 59 46170.0 60 46170.0 61 46170.0 62 37860.0 63 29550.0 64 29550.0 65 29550.0 66 23036.5 67 16523.0 68 16523.0 69 16523.0 70 12129.0 71 7735.0 72 7735.0 73 7735.0 74 5441.5 75 3148.0 76 3148.0 77 3148.0 78 2162.0 79 1176.0 80 1176.0 81 1176.0 82 787.5 83 399.0 84 399.0 85 399.0 86 247.0 87 95.0 88 95.0 89 95.0 90 60.0 91 25.0 92 25.0 93 25.0 94 14.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22486567577924355 2 0.13159289365761387 3 0.13583256557223342 4 0.06767168632950404 5 0.0225028740083652 6 0.02168755248632298 7 0.2931896193263814 8 0.28960220462939557 9 0.017284816267295007 10 0.034406568230181574 11 0.0150019160055768 12 0.016958687658478123 13 0.014512723092351468 14 0.015817237527619016 15 0.014512723092351468 16 0.018589330702562558 17 0.017610944876111895 18 0.013534337265900807 19 0.022176745399548312 20 0.01598030183202746 21 0.013697401570309253 22 0.016306430440844347 23 0.016632559049661235 24 0.014023530179126139 25 0.014349658787943026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613255.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.05849344962043 #Duplication Level Percentage of deduplicated Percentage of total 1 93.24802926906128 81.18032945310586 2 5.09178540131891 8.865663320151905 3 0.8551846334290557 2.233532574227985 4 0.3059660049496935 1.0654775775087773 5 0.1514291370468801 0.6591596267838742 6 0.08958472452998102 0.4679466692207643 7 0.05134851721426406 0.312922718468201 8 0.03590648889850592 0.2500771862855557 9 0.023750922142325458 0.18609475497750771 >10 0.12658386273237784 1.9616136792898655 >50 0.010309294782758466 0.6395162419671964 >100 0.009371985087199186 1.718145177925327 >500 7.497588068100594E-4 0.45952102008714024 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 782 0.1275162860474028 No Hit GTACATGGGAAGCAGTGGTATCAAC 744 0.12131984247988194 No Hit CCCATGTACTCTGCGTTGATACCAC 666 0.10860082673602334 No Hit GTCCTACAGTGGACATTTCTAAATT 624 0.10175212595086873 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.6306430440844348E-4 0.0 7 0.0 0.0 0.0 1.6306430440844348E-4 0.0 8 0.0 0.0 0.0 1.6306430440844348E-4 0.0 9 0.0 0.0 0.0 1.6306430440844348E-4 0.0 10 0.0 0.0 0.0 1.6306430440844348E-4 0.0 11 0.0 0.0 0.0 1.6306430440844348E-4 0.0 12 0.0 0.0 0.0 1.6306430440844348E-4 0.0 13 0.0 0.0 0.0 1.6306430440844348E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCGAG 45 6.847318E-4 14.751074 11 TCCAACG 55 1.9873622E-4 13.793212 18 GTATTAC 90 5.096299E-7 12.724721 1 TATTAGG 60 3.9638608E-4 12.712201 2 CTTACAC 55 0.0029834972 12.134373 3 TAAGATA 55 0.0030111012 12.119468 4 GACGTGG 145 9.458745E-11 11.812252 7 AACAGTG 145 9.458745E-11 11.812252 7 CTCCTAT 65 7.70961E-4 11.745896 1 CTATACC 75 2.025023E-4 11.426926 4 GGCGAGA 195 0.0 11.184879 19 AAATGTC 60 0.005798296 11.10133 7 TAGAACA 200 0.0 10.950805 4 GTCCTAC 340 0.0 10.947002 1 ATTAGGG 70 0.0014452307 10.896172 3 GAACAGT 150 2.062734E-9 10.784148 6 CCCTAGA 90 9.0623E-5 10.603934 1 GTCCTAA 180 3.274181E-11 10.603934 1 TGGCGAG 280 0.0 10.498852 18 TATGGCG 310 0.0 10.400527 16 >>END_MODULE