FastQCFastQC Report
Thu 26 May 2016
SRR1926897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613255
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11770.19192668628873796No Hit
GTACATGGGAAGCAGTGGTATCAAC8490.13844159444276852No Hit
GGTATCAACGCAGAGTACTTTTTTT7940.1294730577003041No Hit
CCCATGTACTCTGCGTTGATACCAC7110.1159387204344033No Hit
TATCAACGCAGAGTACTTTTTTTTT6690.10909001964924869No Hit
GTCCTACAGTGGACATTTCTAAATT6290.10256744747291094No Hit
CTGTAGGACGTGGAATATGGCAAGA6180.10077374012441806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT307.7884266E-418.9734217
CCGGACA400.005181119314.2883174
GTCTAAG500.001455091413.3510661
TAGGACC1551.8189894E-1212.2910264
GTTCTAG701.0505419E-412.2611831
TTAGGAC1950.012.2122353
GACAGTC701.1023075E-412.1981937
CCATTAC550.002978054312.1373321
TATCCGT550.003003755812.1234216
AGGACTG1052.6164707E-711.7935315
GTTCAGA657.757699E-411.7372011
CTTATAC657.832734E-411.7237463
GTCCTAC3300.011.5593641
TAGGACG3250.011.4306534
GTAGGAC3850.011.1337533
CTAAGAC600.00575259511.1131353
CCCTTAG951.2999248E-511.0422351
TTATACT951.3165649E-511.0295784
GGCGAGG1553.1832315E-1011.01682419
GGACGTG3300.010.9688096
Produced by FastQC (version 0.11.5)