##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926897_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613255 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.706161384742074 31.0 30.0 33.0 25.0 34.0 2 30.067689623402988 31.0 30.0 34.0 26.0 34.0 3 30.146368150280065 31.0 30.0 34.0 26.0 34.0 4 33.7641796642506 35.0 35.0 37.0 28.0 37.0 5 33.72307441439532 37.0 35.0 37.0 28.0 37.0 6 33.67350938842733 37.0 35.0 37.0 29.0 37.0 7 33.660126700964526 37.0 35.0 37.0 28.0 37.0 8 33.689186390653155 37.0 35.0 37.0 29.0 37.0 9 35.23470008397812 39.0 35.0 39.0 29.0 39.0 10 35.123511426731135 39.0 35.0 39.0 29.0 39.0 11 35.146345321277444 39.0 35.0 39.0 29.0 39.0 12 35.043066913437315 39.0 35.0 39.0 28.0 39.0 13 35.057359499718714 39.0 35.0 39.0 29.0 39.0 14 36.13881664234291 40.0 36.0 41.0 28.0 41.0 15 36.118084646680416 40.0 36.0 41.0 28.0 41.0 16 36.09547088894506 40.0 36.0 41.0 28.0 41.0 17 36.06198889532087 40.0 36.0 41.0 28.0 41.0 18 36.03302704421488 40.0 36.0 41.0 28.0 41.0 19 36.097783140781566 40.0 36.0 41.0 28.0 41.0 20 36.06180789394298 40.0 36.0 41.0 27.0 41.0 21 36.007181351966146 40.0 36.0 41.0 27.0 41.0 22 35.91148217299492 40.0 36.0 41.0 27.0 41.0 23 35.846421146179 40.0 36.0 41.0 27.0 41.0 24 35.76163912238791 40.0 36.0 41.0 27.0 41.0 25 35.6496172065454 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12202207188586556 1101 2 -0.040543429565982336 1101 3 0.21723920153101162 1101 4 0.24265628006982354 1101 5 0.10177892123174814 1101 6 -0.025716329632899715 1101 7 0.028782765660558596 1101 8 0.025278440174915318 1101 9 0.09126538631638681 1101 10 0.15765361598082706 1101 11 0.32079229171606727 1101 12 0.09382789905097155 1101 13 0.2765204362877043 1101 14 0.07922573664962584 1101 15 -0.05891711033479652 1101 16 0.11239327781103725 1101 17 0.19440174065312021 1101 18 0.007992059147944985 1101 19 0.17243663493268002 1101 20 0.12833953647191976 1101 21 0.19761719448159454 1101 22 -0.10824021706033449 1101 23 -0.09407989075830869 1101 24 0.09944266663499945 1101 25 -0.0705362074373781 1102 1 -0.537377468724312 1102 2 -0.531580533585867 1102 3 -0.1922626287663327 1102 4 -0.3596360645966854 1102 5 -0.41421260669176974 1102 6 -0.2118952877415765 1102 7 -0.37196168421057507 1102 8 -0.04594962406198988 1102 9 -0.28080964378559514 1102 10 -0.2025752558113183 1102 11 -0.31736735276620465 1102 12 -0.06423653128408802 1102 13 -0.24431642304777768 1102 14 -0.38244803037794384 1102 15 -0.3185207312770686 1102 16 -0.16551210766360214 1102 17 -0.029368430094038445 1102 18 -0.35958822489872944 1102 19 -0.21645561205349395 1102 20 -0.38411833283952745 1102 21 -0.5129661311952773 1102 22 -0.5999954166038464 1102 23 -0.2511203700689393 1102 24 -0.29075883532102864 1102 25 -0.1984901912959458 1103 1 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0.45305279516517416 2214 2 0.22985824148791068 2214 3 0.4905375080969314 2214 4 0.23906159589687803 2214 5 0.25288460899618315 2214 6 0.10017647993303314 2214 7 0.028536583613728794 2214 8 0.15710199905079492 2214 9 -0.023151716408662537 2214 10 0.0011649606037238414 2214 11 0.0668848177996395 2214 12 -0.14106600820979764 2214 13 -0.18061085014670653 2214 14 0.02413637576379557 2214 15 -0.2260075795726948 2214 16 -0.19368027840052804 2214 17 -0.2603508517184352 2214 18 -0.2788089596833814 2214 19 -0.08400832579542339 2214 20 -0.20496218040806724 2214 21 -0.037674227512795255 2214 22 -0.08651438499698827 2214 23 -0.043096761155723584 2214 24 -0.14034610703738082 2214 25 0.0905265316842474 2215 1 0.1740178643059913 2215 2 0.11875357998300728 2215 3 0.06850419692111487 2215 4 0.12310069299361714 2215 5 0.29642974947796574 2215 6 0.146049096049353 2215 7 0.023758985800768073 2215 8 0.007297203664549556 2215 9 -0.2940255028164316 2215 10 0.06188529839664625 2215 11 -0.28976103328311353 2215 12 -0.0634092125614103 2215 13 0.005193982688865617 2215 14 -0.13736946164995345 2215 15 -0.20911606090889023 2215 16 -0.36571686639240397 2215 17 -0.2899770138178397 2215 18 -0.33526569166791376 2215 19 -0.3812810786017806 2215 20 -0.14158013174267126 2215 21 -0.09765213241023218 2215 22 -0.03343183731931276 2215 23 -0.0430714828604124 2215 24 0.05096096807660899 2215 25 -0.1730886390378643 2216 1 -0.34367348321549684 2216 2 -0.08272119580545834 2216 3 -1.8310475219629971 2216 4 -1.54955945843966 2216 5 -0.5716940279694356 2216 6 -0.33011497275711577 2216 7 -0.291595470465559 2216 8 -0.2835508397308075 2216 9 -0.2636035204808067 2216 10 -0.3662312111618604 2216 11 -0.29435740520597165 2216 12 -0.020195556501008127 2216 13 -0.3838178777865977 2216 14 -0.4250018427447344 2216 15 -0.49078839457312284 2216 16 -0.4212953969728943 2216 17 -0.6116833021505172 2216 18 -0.48615482513304187 2216 19 -0.5222544646903984 2216 20 -0.3846900689719561 2216 21 -0.2503241698701473 2216 22 -0.2520145422042219 2216 23 -0.21936037387330742 2216 24 -0.48357333342532627 2216 25 -0.3997218557506059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15148.0 3 13.0 4 244.0 5 1647.0 6 2262.0 7 1727.0 8 1292.0 9 1102.0 10 945.0 11 908.0 12 825.0 13 803.0 14 821.0 15 797.0 16 817.0 17 901.0 18 1007.0 19 1101.0 20 1525.0 21 1890.0 22 2527.0 23 3008.0 24 3641.0 25 4215.0 26 5119.0 27 5584.0 28 6372.0 29 7753.0 30 10086.0 31 13135.0 32 16755.0 33 22051.0 34 29980.0 35 41685.0 36 60395.0 37 104786.0 38 240388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18369412609789 18.903745061547 10.690240637346133 28.22232017500898 2 14.667865957974744 20.434095568934218 39.05962284356105 25.838415629529994 3 18.662869966273295 24.148841093084393 29.072483055104342 28.115805885537963 4 12.519261970958247 16.963579587610376 36.87275277005487 33.64440567137651 5 13.50243791073496 37.98101854116726 34.798851980496714 13.717691567601062 6 32.684342901278505 37.09521962746051 17.164536911126273 13.055900560134706 7 28.732057143136743 31.5715993586973 20.90415199461123 18.79219150355473 8 25.838658016706294 37.24489629506411 19.244367903819747 17.672077784409847 9 26.194231029539594 15.815498624377627 19.313835972321204 38.67643437376157 10 15.086241338766937 29.596833767923798 33.36382804894157 21.953096844367693 11 34.992995954123906 22.84917084816631 23.07241971439148 19.085413483318302 12 23.09375652264663 24.99804320601127 30.985995877687326 20.92220439365477 13 29.211251528740316 21.592172849571952 25.26669384427232 23.92988177741541 14 21.311724016476045 21.802548373104784 26.8068369713039 30.078890639115276 15 23.512155189845345 29.985519441128698 22.922822534880535 23.57950283414542 16 22.712959988520158 28.25296086907602 26.590993218089228 22.443085924314595 17 22.111519677784937 28.410367628474287 27.081719676154293 22.396393017586487 18 22.079703090577922 27.907170147296945 28.24210641319786 21.771020348927276 19 23.496664508775332 27.14964296954112 26.80916359153563 22.544528930147916 20 23.505776647180166 27.32333732847394 27.278493913525587 21.892392110820307 21 23.197333163720458 26.617968200847734 26.908756427757147 23.275942207674657 22 23.189211665620338 27.46834514190671 26.682538258962424 22.65990493351053 23 22.997723611174525 27.096528017845845 26.84540776059434 23.060340610385293 24 23.005927387465245 27.016167825782095 27.464431598600907 22.513473188151746 25 23.16796936790316 26.873859490910988 27.086346832177398 22.871824309008453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 193.0 1 193.0 2 184.0 3 175.0 4 175.0 5 175.0 6 298.5 7 422.0 8 422.0 9 422.0 10 682.5 11 943.0 12 943.0 13 943.0 14 1602.0 15 2261.0 16 2261.0 17 2261.0 18 4054.5 19 5848.0 20 5848.0 21 5848.0 22 9492.0 23 13136.0 24 13136.0 25 13136.0 26 18859.5 27 24583.0 28 24583.0 29 24583.0 30 32274.0 31 39965.0 32 39965.0 33 39965.0 34 46598.5 35 53232.0 36 53232.0 37 53232.0 38 58701.0 39 64170.0 40 64170.0 41 64170.0 42 69367.0 43 74564.0 44 74564.0 45 74564.0 46 78739.5 47 82915.0 48 82915.0 49 82915.0 50 81021.0 51 79127.0 52 79127.0 53 79127.0 54 71575.5 55 64024.0 56 64024.0 57 64024.0 58 55527.0 59 47030.0 60 47030.0 61 47030.0 62 38895.0 63 30760.0 64 30760.0 65 30760.0 66 23933.5 67 17107.0 68 17107.0 69 17107.0 70 12520.5 71 7934.0 72 7934.0 73 7934.0 74 5538.0 75 3142.0 76 3142.0 77 3142.0 78 2171.0 79 1200.0 80 1200.0 81 1200.0 82 791.0 83 382.0 84 382.0 85 382.0 86 253.5 87 125.0 88 125.0 89 125.0 90 68.0 91 11.0 92 11.0 93 11.0 94 8.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11659097765203708 2 0.10941614825806557 3 0.014838851701168354 4 0.0 5 0.004076607610211087 6 0.35091438308697037 7 0.020872230964280765 8 0.011903694221816374 9 0.012719015743858591 10 0.005544186349887078 11 0.006196443567520852 12 0.0011414501308591044 13 8.153215220422173E-4 14 1.6306430440844348E-4 15 0.003098221783760426 16 9.78385826450661E-4 17 0.0 18 6.522572176337739E-4 19 3.2612860881688695E-4 20 0.0016306430440844347 21 0.015328044614393686 22 0.0 23 4.891929132253305E-4 24 0.0 25 0.006848700785154626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613255.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.14264703440251 #Duplication Level Percentage of deduplicated Percentage of total 1 91.32490185688805 76.84318982395581 2 6.5265928155252295 10.983295912280138 3 1.1534470875218297 2.9116227347462673 4 0.3696126223253221 1.2440073767911801 5 0.1960923995023143 0.824986677872614 6 0.10599540567757157 0.5351240404317726 7 0.06707956261219612 0.3950976372070086 8 0.04255697158922647 0.28646849914283035 9 0.0338206081208213 0.2561179942439203 >10 0.15624457971153674 2.313922006385544 >50 0.01202200395317418 0.7051560276579616 >100 0.010082875029958346 1.730572427315782 >500 0.0013573100999551932 0.7784068115391417 >1k 1.9390144285074188E-4 0.19203203043000833 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1177 0.19192668628873796 No Hit GTACATGGGAAGCAGTGGTATCAAC 849 0.13844159444276852 No Hit GGTATCAACGCAGAGTACTTTTTTT 794 0.1294730577003041 No Hit CCCATGTACTCTGCGTTGATACCAC 711 0.1159387204344033 No Hit TATCAACGCAGAGTACTTTTTTTTT 669 0.10909001964924869 No Hit GTCCTACAGTGGACATTTCTAAATT 629 0.10256744747291094 No Hit CTGTAGGACGTGGAATATGGCAAGA 618 0.10077374012441806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6306430440844348E-4 0.0 10 0.0 0.0 0.0 1.6306430440844348E-4 0.0 11 0.0 0.0 0.0 3.2612860881688695E-4 0.0 12 0.0 0.0 0.0 3.2612860881688695E-4 1.6306430440844348E-4 13 0.0 0.0 0.0 3.2612860881688695E-4 3.2612860881688695E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGCT 30 7.7884266E-4 18.97342 17 CCGGACA 40 0.0051811193 14.288317 4 GTCTAAG 50 0.0014550914 13.351066 1 TAGGACC 155 1.8189894E-12 12.291026 4 GTTCTAG 70 1.0505419E-4 12.261183 1 TTAGGAC 195 0.0 12.212235 3 GACAGTC 70 1.1023075E-4 12.198193 7 CCATTAC 55 0.0029780543 12.137332 1 TATCCGT 55 0.0030037558 12.123421 6 AGGACTG 105 2.6164707E-7 11.793531 5 GTTCAGA 65 7.757699E-4 11.737201 1 CTTATAC 65 7.832734E-4 11.723746 3 GTCCTAC 330 0.0 11.559364 1 TAGGACG 325 0.0 11.430653 4 GTAGGAC 385 0.0 11.133753 3 CTAAGAC 60 0.005752595 11.113135 3 CCCTTAG 95 1.2999248E-5 11.042235 1 TTATACT 95 1.3165649E-5 11.029578 4 GGCGAGG 155 3.1832315E-10 11.016824 19 GGACGTG 330 0.0 10.968809 6 >>END_MODULE