FastQCFastQC Report
Thu 26 May 2016
SRR1926896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605267
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7720.12754701644067823No Hit
GTCCTACAGTGGACATTTCTAAATT6960.1149905744076581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA451.6959857E-618.96339819
CGTTTCC402.7984814E-416.59297414
TCCAACG651.9499203E-716.04595218
CCGGTTT400.005342073714.222548512
GCCGGTT500.001519622313.27437911
GATATAC1800.012.7200381
CTAATAC603.9681324E-412.7105283
TAAACAG604.0156746E-412.6926035
ACCCTTG604.0156746E-412.6926037
TGCCGGT604.143932E-412.645410
ACATTCG604.1526082E-412.64226618
CATTCGT604.1526082E-412.64226619
TCCTATA853.7794089E-612.3377142
CCTAGAC701.0541857E-412.256583
CATAGAC657.7819085E-411.7327953
GACTGTT657.874625E-411.71624857
TAGGACC1655.456968E-1211.5435194
GGCGAGA1850.011.27553419
ACTGTTC2200.011.2390748
TCTAGGG600.005719488511.1217133
Produced by FastQC (version 0.11.5)