##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926896_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 605267 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.036950635009013 31.0 28.0 31.0 16.0 34.0 2 28.014086345364937 31.0 28.0 31.0 16.0 34.0 3 27.96634708318808 31.0 28.0 31.0 16.0 34.0 4 31.622650830129512 35.0 32.0 37.0 19.0 37.0 5 31.345300503744628 35.0 32.0 37.0 17.0 37.0 6 30.80481836941383 35.0 31.0 37.0 11.0 37.0 7 30.93490971752962 35.0 32.0 37.0 11.0 37.0 8 30.665435254193603 35.0 31.0 37.0 11.0 37.0 9 32.92887766886349 37.0 33.0 39.0 17.0 39.0 10 33.130524214933246 37.0 33.0 39.0 17.0 39.0 11 33.298904450432616 38.0 33.0 39.0 17.0 39.0 12 33.16748971941308 38.0 33.0 39.0 17.0 39.0 13 33.289680422028624 38.0 34.0 39.0 17.0 39.0 14 34.297842109350086 39.0 34.0 41.0 17.0 41.0 15 33.86643580436402 39.0 33.0 41.0 11.0 41.0 16 33.9638308382912 39.0 33.0 40.0 16.0 41.0 17 34.18142902223316 39.0 34.0 41.0 17.0 41.0 18 34.221642680007335 39.0 34.0 41.0 17.0 41.0 19 33.60439442427887 39.0 33.0 40.0 10.0 41.0 20 33.87767216782015 39.0 34.0 41.0 10.0 41.0 21 33.87481722942106 39.0 34.0 41.0 10.0 41.0 22 33.890496260328085 39.0 34.0 41.0 2.0 41.0 23 33.87778121060623 39.0 34.0 41.0 2.0 41.0 24 32.581672220689384 39.0 32.0 40.0 2.0 41.0 25 33.152613639930806 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16177307912225203 1101 2 0.06425380475593556 1101 3 -0.6262610135891187 1101 4 -0.05520055368446464 1101 5 -0.09270050653176654 1101 6 -1.9120008811790825 1101 7 -0.449006042981992 1101 8 0.2323818535258262 1101 9 0.31361266935503807 1101 10 -8.904421099284477 1101 11 -3.329685106657788 1101 12 -1.0067781065674168 1101 13 -0.2938447509093507 1101 14 0.07704230817213187 1101 15 -1.4961572263102312 1101 16 0.09729306177165853 1101 17 0.21569300998154262 1101 18 0.3382062685271663 1101 19 -0.4600631968151987 1101 20 0.3921110643439505 1101 21 0.5478445768184912 1101 22 0.6899042279483254 1101 23 0.5038593127800439 1101 24 0.19742758702928853 1101 25 0.15852149607903954 1102 1 -0.11631999692769313 1102 2 -0.3205362065948023 1102 3 -0.062129345030662364 1102 4 -0.023418487850182856 1102 5 -0.22436906498806408 1102 6 -0.900650143381128 1102 7 0.006158542716079296 1102 8 0.46961227350312384 1102 9 0.28183060352075984 1102 10 0.4849092071854386 1102 11 0.13342499549884934 1102 12 0.13624118968683518 1102 13 -0.02596733887756386 1102 14 -0.34179991657247655 1102 15 0.18829226290656464 1102 16 0.05075503680003379 1102 17 -0.09304705812288461 1102 18 -0.4075372047986008 1102 19 0.16763260341181763 1102 20 0.030022528589128683 1102 21 0.02003526921349419 1102 22 0.24949560138760063 1102 23 -0.21464238983062955 1102 24 -3.200983309678996 1102 25 -1.624679411979983 1103 1 0.30662496615023827 1103 2 -0.07191901473130002 1103 3 0.3994145612189328 1103 4 0.45321340592132486 1103 5 0.06462818355191047 1103 6 0.7774920934606406 1103 7 0.4052978427796532 1103 8 0.4887039056156759 1103 9 0.6284720978029199 1103 10 0.606611766377867 1103 11 0.3743746481559782 1103 12 0.2843451531791601 1103 13 0.5360267360887789 1103 14 0.6173696006479545 1103 15 0.8151657137625037 1103 16 0.9724606846285937 1103 17 0.6744043795085446 1103 18 0.42934677315883363 1103 19 -1.0974624622345672 1103 20 0.0754241928492334 1103 21 0.1516137424491788 1103 22 0.020213271732316684 1103 23 0.08046521053073974 1103 24 1.5797351070861225 1103 25 0.8486141809017411 1104 1 -0.6125642196529988 1104 2 -0.4434879001123413 1104 3 -1.800293535647807 1104 4 -0.7755246454686606 1104 5 -0.8525527588066169 1104 6 -2.064280121871164 1104 7 -0.5695533868466356 1104 8 -0.261733949084217 1104 9 -0.19584105287171383 1104 10 -0.19774297736125135 1104 11 -0.39207528356624977 1104 12 -0.04048289958890905 1104 13 -0.28089487601830854 1104 14 -0.47472771710050665 1104 15 0.14481956425297682 1104 16 -0.39823374415581014 1104 17 -0.46165254786645704 1104 18 -0.5001780386074941 1104 19 -1.6373755176961637 1104 20 -0.5765383232806087 1104 21 -0.4736076905070661 1104 22 -0.397408657333969 1104 23 -0.4446559981737366 1104 24 0.8412790616845385 1104 25 0.1838054213021394 1105 1 0.4649212215893499 1105 2 0.2041513413390561 1105 3 0.7395959671146244 1105 4 0.3291550618629806 1105 5 0.3414744175410007 1105 6 -0.024602654348395703 1105 7 0.3497239014914264 1105 8 0.6824858508261897 1105 9 -0.1879390071796081 1105 10 0.3465664149039469 1105 11 0.13966728773433346 1105 12 0.2752883412652025 1105 13 0.24415813279160403 1105 14 -0.1510424849069878 1105 15 0.49608132324954113 1105 16 0.037298583752210845 1105 17 0.00885066252625677 1105 18 -0.008841619315276716 1105 19 -0.6808098912962848 1105 20 0.144560676271432 1105 21 0.37927569011112183 1105 22 0.4581565211041365 1105 23 0.16972058835754922 1105 24 -5.23760999311612 1105 25 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9 1.1572336024047658 1110 10 1.2288773392736942 1110 11 0.9682455298419868 1110 12 1.0906176621827584 1110 13 0.91856308396126 1110 14 0.7497058684884266 1110 15 1.3186170943147246 1110 16 1.0613128921782788 1110 17 0.9724402605276197 1110 18 0.8365263116408883 1110 19 -0.9245766412395042 1110 20 0.6761811623036849 1110 21 0.18989572190762516 1110 22 0.4373968118150131 1110 23 0.32761407007456 1110 24 -0.5527178741063565 1110 25 0.25409303911169445 1111 1 0.3731207363441875 1111 2 -0.06209365104372466 1111 3 0.6699091195154594 1111 4 0.5767677685153529 1111 5 0.7108701180431716 1111 6 0.5871910600340478 1111 7 0.8445159795820416 1111 8 1.0526906694234945 1111 9 0.5227600843963103 1111 10 0.41216140446552885 1111 11 0.22199984994163202 1111 12 0.277506927283369 1111 13 0.35844352465528573 1111 14 0.5173217875073917 1111 15 -0.5283219944731883 1111 16 -0.1457821587857353 1111 17 0.11436101630383178 1111 18 0.5164069277704328 1111 19 0.9873963943212729 1111 20 0.4427663976504306 1111 21 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-2.410108390522719 2216 12 -2.067008568825404 2216 13 -1.8648627505559254 2216 14 -2.060111898159782 2216 15 -3.2082734613344677 2216 16 -2.0688559375312536 2216 17 -2.335549554309491 2216 18 -1.9539966310605479 2216 19 -1.2059096615536475 2216 20 -2.126282659901417 2216 21 -4.625936628224405 2216 22 -3.3146729272553976 2216 23 -3.156099902620536 2216 24 -3.5443024735621833 2216 25 -2.859768694693578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36752.0 3 3.0 4 294.0 5 1736.0 6 2217.0 7 1738.0 8 1332.0 9 1224.0 10 1129.0 11 1163.0 12 1186.0 13 1095.0 14 1125.0 15 1159.0 16 1333.0 17 1342.0 18 1511.0 19 1889.0 20 2468.0 21 3172.0 22 3943.0 23 4787.0 24 5663.0 25 6351.0 26 7309.0 27 7997.0 28 8993.0 29 10543.0 30 12869.0 31 15368.0 32 19824.0 33 25914.0 34 35455.0 35 49625.0 36 71531.0 37 114262.0 38 140965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8528580038014 18.10830678755207 12.561342278323409 25.47749293032312 2 14.799191405875211 19.577969449434747 41.56981072148167 24.05302842320837 3 19.57230556975469 23.648421745682775 29.31677080283277 27.462501881729768 4 11.825644162650553 16.158252804391136 38.626282332129186 33.38982070082913 5 13.476859112173855 37.543316351065286 35.76587935704241 13.213945179718445 6 32.24808020583564 36.590855384595045 17.904032600558875 13.257031809010433 7 27.98456074524732 31.00877200251244 22.319965130751395 18.68670212148884 8 24.91809744125967 36.96162635199292 21.02555094695432 17.094725259793094 9 25.764831467729515 15.693379998215365 19.87917364822214 38.66261488583298 10 14.466664463028462 29.59260238654018 34.50269394770766 21.438039202723697 11 35.01074060610067 22.76297960937242 23.340493737400443 18.885786047126473 12 22.642301343243382 24.923452147057805 31.70593066160809 20.728315848090723 13 29.019417331343845 21.661453350319988 25.40198717408511 23.91714214425106 14 20.697159475849762 22.017119156600625 27.448816035163677 29.836905332385943 15 23.48108429653742 30.39715128431802 23.03229590951527 23.089468509629288 16 22.244640646972606 28.51557529698905 27.286142273823156 21.953641782215193 17 21.93295404409031 28.469496849629937 27.317087679929674 22.280461426350083 18 21.567914657642444 28.326993684586228 28.619465751025302 21.485625906746026 19 23.761648150259692 27.040894736581183 26.941578711667898 22.255878401491227 20 23.09534117460433 27.708890335672052 27.784075323791175 21.411693165932444 21 23.110326776649746 27.031944003807105 27.072923276226735 22.784805943316414 22 22.668980047093815 27.729004007105384 27.390259015987112 22.21175692981369 23 22.720355073194188 27.433072635668264 27.157778545237626 22.68879374589992 24 23.086900986974186 27.285961436458063 27.51729642108255 22.1098411554852 25 22.847927241962775 27.140671266920474 27.520720970812185 22.49068052030457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 166.0 2 217.5 3 269.0 4 269.0 5 269.0 6 416.5 7 564.0 8 564.0 9 564.0 10 861.5 11 1159.0 12 1159.0 13 1159.0 14 1938.5 15 2718.0 16 2718.0 17 2718.0 18 4620.0 19 6522.0 20 6522.0 21 6522.0 22 10162.5 23 13803.0 24 13803.0 25 13803.0 26 19986.5 27 26170.0 28 26170.0 29 26170.0 30 34427.5 31 42685.0 32 42685.0 33 42685.0 34 49080.0 35 55475.0 36 55475.0 37 55475.0 38 60757.5 39 66040.0 40 66040.0 41 66040.0 42 70706.0 43 75372.0 44 75372.0 45 75372.0 46 77809.5 47 80247.0 48 80247.0 49 80247.0 50 78241.0 51 76235.0 52 76235.0 53 76235.0 54 68418.0 55 60601.0 56 60601.0 57 60601.0 58 52033.0 59 43465.0 60 43465.0 61 43465.0 62 35600.5 63 27736.0 64 27736.0 65 27736.0 66 21424.5 67 15113.0 68 15113.0 69 15113.0 70 10924.0 71 6735.0 72 6735.0 73 6735.0 74 4711.0 75 2687.0 76 2687.0 77 2687.0 78 1878.5 79 1070.0 80 1070.0 81 1070.0 82 682.5 83 295.0 84 295.0 85 295.0 86 200.0 87 105.0 88 105.0 89 105.0 90 66.5 91 28.0 92 28.0 93 28.0 94 16.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21131170210832573 2 0.12572963667274112 3 0.12721658375559877 4 0.06806913312637232 5 0.02081725916000707 6 0.020486826474927593 7 0.3089545605493113 8 0.298380714626768 9 0.016686850596513605 10 0.0341997829057259 11 0.014373821800957262 12 0.014869470828576481 13 0.014208605458417524 14 0.01602598522635465 15 0.01354774008825857 16 0.018173797679371252 17 0.016521634253973867 18 0.01338252374571883 19 0.02114769184508655 20 0.015365119856195696 21 0.013712956430798309 22 0.015199903513655955 23 0.015860768883814914 24 0.01387817277333805 25 0.013712956430798309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 605267.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.86562188612619 #Duplication Level Percentage of deduplicated Percentage of total 1 94.04160835006545 82.63024400850017 2 4.51151816981706 7.928146992830676 3 0.7159832284973872 1.887309348958779 4 0.255931074541376 0.8995017209825007 5 0.13231469882547633 0.5812956648487984 6 0.0810867262955827 0.42748413796028845 7 0.04996039379451911 0.3072860749301827 8 0.03584818051092686 0.25198581392629577 9 0.02281516790786984 0.18042020249952345 >10 0.13354892090229828 2.0825228774314444 >50 0.009222077348495611 0.5743220907495782 >100 0.009221991910999901 1.7361833210640418 >500 9.410195825845922E-4 0.5132977453177187 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 772 0.12754701644067823 No Hit GTCCTACAGTGGACATTTCTAAATT 696 0.1149905744076581 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6521634253973866E-4 0.0 10 0.0 0.0 0.0 1.6521634253973866E-4 0.0 11 0.0 0.0 0.0 1.6521634253973866E-4 0.0 12 0.0 0.0 0.0 1.6521634253973866E-4 0.0 13 0.0 0.0 0.0 1.6521634253973866E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 45 1.6959857E-6 18.963398 19 CGTTTCC 40 2.7984814E-4 16.592974 14 TCCAACG 65 1.9499203E-7 16.045952 18 CCGGTTT 40 0.0053420737 14.2225485 12 GCCGGTT 50 0.0015196223 13.274379 11 GATATAC 180 0.0 12.720038 1 CTAATAC 60 3.9681324E-4 12.710528 3 TAAACAG 60 4.0156746E-4 12.692603 5 ACCCTTG 60 4.0156746E-4 12.692603 7 TGCCGGT 60 4.143932E-4 12.6454 10 ACATTCG 60 4.1526082E-4 12.642266 18 CATTCGT 60 4.1526082E-4 12.642266 19 TCCTATA 85 3.7794089E-6 12.337714 2 CCTAGAC 70 1.0541857E-4 12.25658 3 CATAGAC 65 7.7819085E-4 11.732795 3 GACTGTT 65 7.874625E-4 11.7162485 7 TAGGACC 165 5.456968E-12 11.543519 4 GGCGAGA 185 0.0 11.275534 19 ACTGTTC 220 0.0 11.239074 8 TCTAGGG 60 0.0057194885 11.121713 3 >>END_MODULE