FastQCFastQC Report
Thu 26 May 2016
SRR1926896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605267
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13680.22601595659436247No Hit
GGTATCAACGCAGAGTACTTTTTTT8810.14555559777750976No Hit
TATCAACGCAGAGTACTTTTTTTTT7000.11565143977781706No Hit
CTGTAGGACGTGGAATATGGCAAGA6260.1034254304298764No Hit
GTACATGGGAAGCAGTGGTATCAAC6120.10111240163432006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1101.891749E-1013.79894918
TAGGACC2050.013.0145514
GTAGGAC3750.012.7057363
CTGTAGG4300.012.4164371
TGGACTG701.05872685E-412.2509435
ATCCCGT701.1047287E-412.19527110
CCCGTTT701.1055813E-412.194262512
TTAGGCA550.00299667712.1271954
CGTGGAA1459.822543E-1111.776699
ACGTGGA1459.822543E-1111.7757168
GTTATAA657.77401E-411.7342171
GGACCAT657.812024E-411.7273986
TGTAGGA4150.011.7145972
TGGCGAG3350.011.61068318
CCAACGA907.5556345E-611.59495119
GGCGAGG1406.91216E-1011.51965919
TAAGGCC752.0129821E-411.4342134
GTAGAAC2700.011.2939873
TGGAATA5050.011.26955711
GTGCTAG855.120025E-511.216531
Produced by FastQC (version 0.11.5)