FastQCFastQC Report
Thu 26 May 2016
SRR1926895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691757
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15690.2268137510715468No Hit
GTCCTAAAGTGTGTATTTCTCATTT12210.17650706823349818No Hit
CTGTAGGACGTGGAATATGGCAAGA12080.1746277955987435No Hit
GTCCTACAGTGTGCATTTCTCATTT10920.15785890131939395No Hit
CTTTAGGACGTGAAATATGGCGAGG10720.15496771265054057No Hit
GTATCAACGCAGAGTACTTTTTTTT9200.132994678767255No Hit
CTGTAGGACCTGGAATATGGCGAGA7600.10986516941642803No Hit
GTACATGGGAAGCAGTGGTATCAAC7470.10798589678167334No Hit
CCCATGTACTCTGCGTTGATACCAC6960.10061336567609724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2350.014.5935874
TCTAGGA801.2266901E-714.3041012
TCCAACG802.0387251E-613.03719818
AGGACCT4500.012.4792515
GTTCTAG550.002957017612.1491811
AATGCCG951.0615586E-611.97675618
ATGCCGG951.0615586E-611.97675619
CCTATTC802.75414E-511.9192173
GTTTATA907.055558E-611.667071
TGTAGGA8750.011.5522652
GGACGTG6650.011.4494826
CTTAGGC752.0003477E-411.4424493
CTAGGAC752.0003477E-411.4424493
TATACTG1251.7733328E-811.4216865
GACGTGG3350.011.3640387
GGACCTG4300.011.2880576
TAGAACC855.1647345E-511.2074944
AATCCCG1105.078E-711.20552619
AGGACGT6800.011.1977335
TCTAGAC600.005709833511.1246033
Produced by FastQC (version 0.11.5)