FastQCFastQC Report
Thu 26 May 2016
SRR1926895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691757
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15940.2304277369076135No Hit
GTATCAACGCAGAGTACTTTTTTTT15090.2181401850649867No Hit
CTGTAGGACGTGGAATATGGCAAGA14740.2130806048944933No Hit
CTTTAGGACGTGAAATATGGCGAGG12650.1828676833049756No Hit
GTCCTACAGTGTGCATTTCTCATTT11840.17115836919611946No Hit
GTCCTAAAGTGTGTATTTCTCATTT11640.16826718052726608No Hit
GGTATCAACGCAGAGTACTTTTTTT9700.1402226504393884No Hit
GTACATGGGAAGCAGTGGTATCAAC9290.134295713668239No Hit
CTGTAGGACCTGGAATATGGCGAGA8940.1292361334977456No Hit
TATCAACGCAGAGTACTTTTTTTTT8240.11911697315675882No Hit
CCCATGTACTCTGCGTTGATACCAC8160.11796049768921744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA250.00588032819.0821651
TAGGACC3050.014.9947584
TCCAACG851.7431375E-814.50890118
TAGGATA801.2395867E-714.2918794
CCAACGT551.981275E-413.79867619
GATATAC2800.013.2893651
GCCTCGA500.001514111613.28122416
CTCGAAC500.001514111613.28122418
CCGTGCC500.001516588313.2783459
TTAAGAC603.986953E-412.7038913
AATCCCG1501.8189894E-1212.64878619
ACTGTTC3050.012.438738
GTCTTAG853.7489826E-612.3472821
CACTGTT3100.012.2389917
TAACACC550.00299882112.1264434
GACGTGG3700.012.048797
GTCCTAA3900.011.9875131
AGGACCT5600.011.7397585
GTAGGAC8450.011.6139133
GGACCTG5200.011.5434416
Produced by FastQC (version 0.11.5)