FastQCFastQC Report
Thu 26 May 2016
SRR1926894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641821
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18780.29260494748535804No Hit
GTCCTAAAGTGTGTATTTCTCATTT15680.24430487628170472No Hit
CTGTAGGACGTGGAATATGGCAAGA14880.2318403417775361No Hit
CTTTAGGACGTGAAATATGGCGAGG12200.19008415118857128No Hit
GTCCTACAGTGTGCATTTCTCATTT12140.18914931110075864No Hit
GTATCAACGCAGAGTACTTTTTTTT10140.1579879748403371No Hit
GTACATGGGAAGCAGTGGTATCAAC9380.1461466670613769No Hit
CTGTAGGACCTGGAATATGGCGAGA8180.12744986530512403No Hit
CCCATGTACTCTGCGTTGATACCAC8120.12651502521731137No Hit
CTGAAGGACCTGGAATATGGCGAGA6600.10283240965939101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCC402.711587E-416.6619724
ATTAGGC400.0051490514.3018333
TAGGACC3500.014.1456754
TAGGGTG500.001477347513.3243645
GTATAAG655.217964E-513.2130811
TCCTAGA1051.8848368E-812.7137372
TAATACT853.8365833E-612.3214594
ACCAGTC701.07051674E-412.2366617
TTCTAGG550.002981057412.1358392
GTCCTAG1209.322321E-911.9284761
GTTCTAG802.7316046E-511.9284751
TTATAAC802.755831E-511.9181953
CTAGACA802.7958895E-511.9014084
GCAGAAC752.0015125E-411.4414663
AGGACCT6450.011.3618615
GTCCTAC9800.011.2955371
GGACCTG6200.011.2059746
GTAGGAC9450.011.1993193
TTAGGAC4350.011.1784443
TTAGAAC600.005712718211.1236493