##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926894_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 641821 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.244731786588474 31.0 28.0 31.0 16.0 34.0 2 28.256107232390338 31.0 28.0 31.0 18.0 34.0 3 28.17745913580266 31.0 28.0 33.0 16.0 34.0 4 31.829134602950045 35.0 32.0 37.0 19.0 37.0 5 31.54867790240581 35.0 32.0 37.0 18.0 37.0 6 31.036391766551734 35.0 32.0 37.0 16.0 37.0 7 31.173288190944206 35.0 32.0 37.0 17.0 37.0 8 30.916302208871322 35.0 32.0 37.0 11.0 37.0 9 33.10962402289735 37.0 33.0 39.0 17.0 39.0 10 33.281586298983676 37.0 33.0 39.0 17.0 39.0 11 33.44943060448318 38.0 34.0 39.0 18.0 39.0 12 33.3270709434562 38.0 34.0 39.0 17.0 39.0 13 33.44103885662825 38.0 34.0 39.0 18.0 39.0 14 34.4507970290782 39.0 34.0 41.0 17.0 41.0 15 34.03538369732371 39.0 33.0 41.0 14.0 41.0 16 34.12267750665684 39.0 34.0 41.0 17.0 41.0 17 34.354341786884504 39.0 34.0 41.0 17.0 41.0 18 34.39339005735244 39.0 34.0 41.0 17.0 41.0 19 33.76584125480469 39.0 33.0 41.0 10.0 41.0 20 34.04611877766543 39.0 34.0 41.0 11.0 41.0 21 34.04286553415984 39.0 34.0 41.0 10.0 41.0 22 34.07182376394665 39.0 34.0 41.0 10.0 41.0 23 34.0614439228383 39.0 34.0 41.0 2.0 41.0 24 32.76998571252732 39.0 32.0 41.0 2.0 41.0 25 33.34913316952858 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.46455598412585886 1101 2 -0.6220686023344832 1101 3 -1.1580983565204477 1101 4 -0.6412344833843981 1101 5 -1.0026107468467416 1101 6 -2.797306425017311 1101 7 -1.1701289507623436 1101 8 -0.7174355191981121 1101 9 -0.9882342960545287 1101 10 -9.443840883090125 1101 11 -4.490201876870632 1101 12 -2.3290755048676317 1101 13 -1.4619322090218034 1101 14 -1.184571468772127 1101 15 -2.6586499247461894 1101 16 -1.1036693725474294 1101 17 -0.9929754467285647 1101 18 -0.7886284063317106 1101 19 -1.3462098532701745 1101 20 -0.7863440342705204 1101 21 -0.9196600319532493 1101 22 -0.6344837418979168 1101 23 -0.700517045955003 1101 24 -0.6769382392593855 1101 25 -0.9068212211820921 1102 1 -0.42305811337976706 1102 2 -0.28706933271485013 1102 3 -0.4656275187685921 1102 4 -0.2907154672903829 1102 5 -0.3703578189135399 1102 6 -1.2785412250213248 1102 7 -0.19958948401806964 1102 8 0.37860336602958355 1102 9 0.29494142008063307 1102 10 0.2855214112185962 1102 11 0.10239083962024154 1102 12 0.14317059874825588 1102 13 -0.14059941538938148 1102 14 -0.43329218804625214 1102 15 0.07882683967993387 1102 16 0.02281801082866508 1102 17 -0.10651112330052825 1102 18 -0.21502000394676912 1102 19 0.37632254829852485 1102 20 0.1916207803522525 1102 21 0.12733074518261134 1102 22 -0.26986448193490986 1102 23 -0.6029794871203578 1102 24 -3.365531928103966 1102 25 -1.7982331746652278 1103 1 0.19348143215984237 1103 2 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21 -0.5796898708107747 1108 22 -0.44564233669002107 1108 23 -0.3392898597213332 1108 24 -1.5064521566841371 1108 25 -0.9588917133757349 1109 1 0.395984381005956 1109 2 0.2426372983728804 1109 3 -0.9632417788745542 1109 4 0.3156935667393199 1109 5 0.6426821979528938 1109 6 0.926267386088913 1109 7 1.1576253731259847 1109 8 1.4598790052599782 1109 9 0.6213005680741261 1109 10 0.8915549363970925 1109 11 0.5972244461939553 1109 12 0.5003403528738701 1109 13 0.5201388266208227 1109 14 0.6577403878710442 1109 15 -0.9680509300911808 1109 16 0.20089836342938128 1109 17 0.2275795211796492 1109 18 0.41726138014948333 1109 19 -1.8201073904557745 1109 20 -0.06790303666375053 1109 21 0.30502614876546374 1109 22 0.566535332198697 1109 23 0.7462343043337754 1109 24 0.07091424773697241 1109 25 0.13127080751173992 1110 1 0.5581469151974439 1110 2 0.7082324514897849 1110 3 0.9751024387081806 1110 4 0.6245869915514746 1110 5 0.7807145380370173 1110 6 0.9133350886765506 1110 7 0.9446785240522821 1110 8 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-1.8502118784197208 2216 15 -3.0757845296275192 2216 16 -1.8694232924410699 2216 17 -2.164948328664302 2216 18 -2.013624567586234 2216 19 -1.260095616092073 2216 20 -2.2293809387061643 2216 21 -4.981110086473155 2216 22 -3.4161971246393037 2216 23 -3.297340897101087 2216 24 -3.401286187036895 2216 25 -2.798365485318879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36488.0 3 3.0 4 332.0 5 1879.0 6 2298.0 7 1819.0 8 1461.0 9 1336.0 10 1205.0 11 1243.0 12 1203.0 13 1179.0 14 1182.0 15 1206.0 16 1281.0 17 1386.0 18 1617.0 19 2019.0 20 2583.0 21 3244.0 22 4176.0 23 4973.0 24 5875.0 25 6668.0 26 7682.0 27 8479.0 28 9423.0 29 11273.0 30 13193.0 31 16075.0 32 20768.0 33 26876.0 34 36893.0 35 51494.0 36 75413.0 37 119798.0 38 157798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.274926065761655 19.24632358681769 13.083939953219224 25.394810394201432 2 13.751583492982707 20.135322346128167 43.35276167416551 22.76033248672362 3 18.724345643264225 25.14708107829815 30.036210573612305 26.092362704825323 4 11.518324607329843 17.133578014836804 39.69281765199305 31.655279725840302 5 12.729318480747533 38.34021106962305 36.541194731363106 12.389275718266312 6 30.45979161017451 37.687359159209706 18.88817202290231 12.964677207713478 7 26.710064127404777 31.34226908509069 23.890114629298598 18.057552158205933 8 23.53231599949378 38.417190202472035 22.03584469071416 16.01464910732002 9 25.95542493112089 15.690879283906597 20.931613329510178 37.42208245546233 10 14.52273297579025 29.7194050508026 35.230618406143385 20.527243567263767 11 33.40730063500721 22.639760021816198 25.723791343644088 18.22914799953251 12 23.327572746241724 25.085980268560753 32.658477179192694 18.92796980600483 13 28.803546662926777 22.806476243903198 25.941595375001945 22.448381718168076 14 19.89531161857151 23.499097728894807 27.950457680524345 28.65513297200934 15 22.28074467571202 32.78393215827865 23.300489770604102 21.634833395405227 16 20.603278198019616 28.507824601757225 29.98983939385439 20.899057806368766 17 20.674329597446796 29.64061311757446 28.891046339122088 20.794010945856655 18 20.606797251180403 27.930749692238166 31.39123930625029 20.071213750331136 19 23.49814235310252 27.040294730017827 27.695769801393862 21.765793115485792 20 22.98543746055492 28.223900017920727 28.785987548989816 20.004674972534538 21 22.04310542300566 27.3258649679226 27.547142173721305 23.083887435350437 22 22.282633256196284 29.36615164052578 27.681291539078874 20.66992356419906 23 21.129146369209575 28.81506484277847 28.61964825013324 21.436140537878718 24 23.110510642475468 27.50603455395725 29.009335832861943 20.37411897070534 25 21.826049828275085 28.132343499697686 28.874562896198363 21.167043775828862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 295.0 3 355.0 4 355.0 5 355.0 6 552.5 7 750.0 8 750.0 9 750.0 10 1099.5 11 1449.0 12 1449.0 13 1449.0 14 2362.5 15 3276.0 16 3276.0 17 3276.0 18 5832.5 19 8389.0 20 8389.0 21 8389.0 22 13242.0 23 18095.0 24 18095.0 25 18095.0 26 26528.0 27 34961.0 28 34961.0 29 34961.0 30 45764.5 31 56568.0 32 56568.0 33 56568.0 34 63150.5 35 69733.0 36 69733.0 37 69733.0 38 73660.0 39 77587.0 40 77587.0 41 77587.0 42 79626.5 43 81666.0 44 81666.0 45 81666.0 46 82940.0 47 84214.0 48 84214.0 49 84214.0 50 78939.5 51 73665.0 52 73665.0 53 73665.0 54 63543.5 55 53422.0 56 53422.0 57 53422.0 58 44668.0 59 35914.0 60 35914.0 61 35914.0 62 28941.5 63 21969.0 64 21969.0 65 21969.0 66 16682.5 67 11396.0 68 11396.0 69 11396.0 70 8305.5 71 5215.0 72 5215.0 73 5215.0 74 3591.0 75 1967.0 76 1967.0 77 1967.0 78 1331.5 79 696.0 80 696.0 81 696.0 82 455.5 83 215.0 84 215.0 85 215.0 86 138.0 87 61.0 88 61.0 89 61.0 90 39.0 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21610386696602324 2 0.12978696552465563 3 0.13212406574418725 4 0.06902235981683368 5 0.021345515338388743 6 0.021657128700992956 7 0.2874633270023885 8 0.27764750608035577 9 0.018229381712346587 10 0.03427746988646367 11 0.01495744140500233 12 0.01620389485541919 13 0.014178407998491791 14 0.016671314899325513 15 0.014334214679793898 16 0.019787448525367665 17 0.018229381712346587 18 0.014178407998491791 19 0.02321519551401403 20 0.016515508218023407 21 0.014334214679793898 22 0.016515508218023407 23 0.017917768349742375 24 0.01558066813021076 25 0.015113248086304437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 641821.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.13287333946 #Duplication Level Percentage of deduplicated Percentage of total 1 93.46883306680827 77.70332660530104 2 4.835589519306986 8.039929020603362 3 0.7876542374417298 1.964398798695969 4 0.29536392420074953 0.9821780679850712 5 0.15979241352353407 0.6642001237029288 6 0.09323040597907105 0.46503069189867297 7 0.06588553460158282 0.38340776620551964 8 0.041918737992697575 0.278786010887755 9 0.03176343180688935 0.23765268179058371 >10 0.17909861606168243 2.805942359689594 >50 0.020247689015204896 1.1961945591224792 >100 0.01818522778590198 3.1998521005639193 >500 0.001312336025410297 0.7727274225484211 >1k 0.001124859450351683 1.3063737910046118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1878 0.29260494748535804 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1568 0.24430487628170472 No Hit CTGTAGGACGTGGAATATGGCAAGA 1488 0.2318403417775361 No Hit CTTTAGGACGTGAAATATGGCGAGG 1220 0.19008415118857128 No Hit GTCCTACAGTGTGCATTTCTCATTT 1214 0.18914931110075864 No Hit GTATCAACGCAGAGTACTTTTTTTT 1014 0.1579879748403371 No Hit GTACATGGGAAGCAGTGGTATCAAC 938 0.1461466670613769 No Hit CTGTAGGACCTGGAATATGGCGAGA 818 0.12744986530512403 No Hit CCCATGTACTCTGCGTTGATACCAC 812 0.12651502521731137 No Hit CTGAAGGACCTGGAATATGGCGAGA 660 0.10283240965939101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.558066813021076E-4 0.0 11 0.0 0.0 0.0 1.558066813021076E-4 0.0 12 0.0 0.0 0.0 1.558066813021076E-4 0.0 13 0.0 0.0 0.0 1.558066813021076E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATTCC 40 2.711587E-4 16.661972 4 ATTAGGC 40 0.00514905 14.301833 3 TAGGACC 350 0.0 14.145675 4 TAGGGTG 50 0.0014773475 13.324364 5 GTATAAG 65 5.217964E-5 13.213081 1 TCCTAGA 105 1.8848368E-8 12.713737 2 TAATACT 85 3.8365833E-6 12.321459 4 ACCAGTC 70 1.07051674E-4 12.236661 7 TTCTAGG 55 0.0029810574 12.135839 2 GTCCTAG 120 9.322321E-9 11.928476 1 GTTCTAG 80 2.7316046E-5 11.928475 1 TTATAAC 80 2.755831E-5 11.918195 3 CTAGACA 80 2.7958895E-5 11.901408 4 GCAGAAC 75 2.0015125E-4 11.441466 3 AGGACCT 645 0.0 11.361861 5 GTCCTAC 980 0.0 11.295537 1 GGACCTG 620 0.0 11.205974 6 GTAGGAC 945 0.0 11.199319 3 TTAGGAC 435 0.0 11.178444 3 TTAGAAC 60 0.0057127182 11.123649 3 >>END_MODULE