FastQCFastQC Report
Thu 26 May 2016
SRR1926894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641821
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19030.29650011451791075No Hit
CTGTAGGACGTGGAATATGGCAAGA18930.2949420477048897No Hit
CTTTAGGACGTGAAATATGGCGAGG16450.25630199074196697No Hit
GTATCAACGCAGAGTACTTTTTTTT15550.2422793894247773No Hit
GTCCTAAAGTGTGTATTTCTCATTT15360.2393190624800373No Hit
GTCCTACAGTGTGCATTTCTCATTT11970.18650059751862277No Hit
GTACATGGGAAGCAGTGGTATCAAC10360.16141572182898348No Hit
GGTATCAACGCAGAGTACTTTTTTT10010.1559624879834097No Hit
CTGTAGGACCTGGAATATGGCGAGA9770.15222312763215912No Hit
CCCATGTACTCTGCGTTGATACCAC9200.143342146797939No Hit
CTGAAGGACCTGGAATATGGCGAGA9110.14193988666622No Hit
TATCAACGCAGAGTACTTTTTTTTT8480.13212406574418725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC508.534691E-515.2344663
TACAGCG508.534691E-515.2344665
GTCCTAG759.2883784E-713.9802281
GGCGAGG4650.013.26404919
GCTCTAT603.9719656E-412.7092981
TCCAACG751.4932279E-512.65186218
GTATTAG853.7880436E-612.3354961
CTTCTAG701.05533334E-412.2553961
CTGATCA701.1009289E-412.200019
CTACTAG550.002988870712.1316041
TCTAGCC550.003013497512.1183243
GACGTGA5300.011.8191257
GTAATAC657.861302E-411.7188193
GTCCTAA4800.011.7163851
CCAACGA658.091147E-411.67864219
TAGGACC3600.011.6374384
TAAGACC907.255998E-611.6374384
GTATAAG752.0065607E-411.4383691
CTAGACA752.0270857E-411.4258484
AGGACGT10850.011.3205415
Produced by FastQC (version 0.11.5)