##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926894_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 641821 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.756034782283535 31.0 30.0 33.0 25.0 34.0 2 30.01747216124122 31.0 30.0 33.0 26.0 34.0 3 30.115691135067255 31.0 30.0 34.0 26.0 34.0 4 33.703414191807376 35.0 35.0 37.0 28.0 37.0 5 33.68137377867038 37.0 35.0 37.0 28.0 37.0 6 33.65309174988042 37.0 35.0 37.0 29.0 37.0 7 33.64071758325141 37.0 35.0 37.0 28.0 37.0 8 33.67990919586614 37.0 35.0 37.0 29.0 37.0 9 35.19607336001783 39.0 35.0 39.0 29.0 39.0 10 35.075985360404225 39.0 35.0 39.0 28.0 39.0 11 35.11343817045562 39.0 35.0 39.0 29.0 39.0 12 35.00974726598226 39.0 35.0 39.0 28.0 39.0 13 35.02537779225049 39.0 35.0 39.0 28.0 39.0 14 36.09218458105921 40.0 36.0 41.0 28.0 41.0 15 36.08945173186917 40.0 36.0 41.0 28.0 41.0 16 36.07126441796077 40.0 36.0 41.0 28.0 41.0 17 36.05365047263957 40.0 36.0 41.0 28.0 41.0 18 36.01746748704078 40.0 36.0 41.0 27.0 41.0 19 36.086013078412826 40.0 36.0 41.0 27.0 41.0 20 36.042380975380986 40.0 36.0 41.0 27.0 41.0 21 35.984818196973926 40.0 36.0 41.0 27.0 41.0 22 35.898340814650815 40.0 36.0 41.0 27.0 41.0 23 35.82368292717128 40.0 36.0 41.0 27.0 41.0 24 35.75775800417873 40.0 36.0 41.0 27.0 41.0 25 35.64263556349823 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.016311253982422613 1101 2 -0.07139750513136889 1101 3 0.09136138598460519 1101 4 0.17596268557021943 1101 5 -0.033794592438077586 1101 6 0.02801350406050318 1101 7 -0.04288822384858548 1101 8 -0.07959512631433796 1101 9 0.155901342683336 1101 10 -0.030490862522256634 1101 11 0.014150237104466612 1101 12 -0.23130742387436953 1101 13 -0.19916841124528872 1101 14 0.005384619487166731 1101 15 -0.042478153298269206 1101 16 0.14637982256336812 1101 17 0.022034490868691137 1101 18 0.15285276515094637 1101 19 0.060154785081898865 1101 20 0.11271403052881368 1101 21 0.12451561672626354 1101 22 -0.21672952536660262 1101 23 -0.22074036592513124 1101 24 -0.28648494276740166 1101 25 -0.10612592325330894 1102 1 -0.7112085156693446 1102 2 -0.5822924385217938 1102 3 -0.33340330330266355 1102 4 -0.5579380027714649 1102 5 -0.5174948443356726 1102 6 -0.4606410683993616 1102 7 -0.4760350035402183 1102 8 -0.651856506588274 1102 9 -0.4545383016939013 1102 10 -0.201285014572953 1102 11 -0.6957504869199198 1102 12 -0.5348200516491204 1102 13 -0.4566001708598293 1102 14 -0.45232635729342263 1102 15 -0.5727097675057848 1102 16 -0.5510888240473903 1102 17 -0.6765938436522063 1102 18 -0.6563640775318191 1102 19 -0.6327163680158634 1102 20 -0.5843453800598084 1102 21 -0.596328546865557 1102 22 -0.40976961377050714 1102 23 -0.4155188711097182 1102 24 -0.27364920475427823 1102 25 -0.7065565688983995 1103 1 -0.5933981369145442 1103 2 -0.8452454670290628 1103 3 -0.3098217198932467 1103 4 -0.6166968847104357 1103 5 -0.7292584160518345 1103 6 -0.5287806388906233 1103 7 -0.5101768083388762 1103 8 -0.4381886698401516 1103 9 -0.5128571271513991 1103 10 -0.4766061253753193 1103 11 -0.5140559292163829 1103 12 -0.5451307564767021 1103 13 -0.5002553616549363 1103 14 -0.4718805165643829 1103 15 -0.49704160184946744 1103 16 -0.37065319081405335 1103 17 -0.4566960320258673 1103 18 -0.38744987153321375 1103 19 -0.6796901290810737 1103 20 -0.9093758607761444 1103 21 -0.4974224501552058 1103 22 -0.46172803523800354 1103 23 -0.5708497357413123 1103 24 -0.470219486197621 1103 25 -0.6498822844154972 1104 1 -0.45537523148221837 1104 2 -0.6325795822071392 1104 3 -0.17659425357523162 1104 4 -0.6107455124606815 1104 5 -0.46004607786844787 1104 6 -0.5872012751027569 1104 7 -0.4816454606667335 1104 8 -0.4364570146673543 1104 9 -0.5278225333272246 1104 10 -0.4502025924845299 1104 11 -0.48022636762927107 1104 12 -0.36923701254394814 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19 0.04045818894535813 1108 20 0.11777041037578329 1108 21 0.11105546824845192 1108 22 -0.0705960386244584 1108 23 0.12554947980207487 1108 24 0.1281076493569131 1108 25 0.12103923777326742 1109 1 0.7672306681548271 1109 2 0.479348702983156 1109 3 0.6196979487781036 1109 4 0.7496645829950737 1109 5 0.7797267048376355 1109 6 0.7134828054674287 1109 7 0.7879036647459614 1109 8 0.672450978519862 1109 9 0.6824437609405507 1109 10 0.6415582377532587 1109 11 0.5797020379575386 1109 12 0.7752139014835961 1109 13 0.5478010446140757 1109 14 0.7236446425146212 1109 15 0.5579798996024721 1109 16 0.5245667693037959 1109 17 0.5438434878014817 1109 18 0.5358261774890423 1109 19 0.515477465877062 1109 20 0.5389778103389133 1109 21 0.7587098274118773 1109 22 0.609952992177007 1109 23 0.7145425004625849 1109 24 0.5253046149993708 1109 25 0.7124158584726246 1110 1 0.6215921353555771 1110 2 0.2629173209489508 1110 3 0.35582716753086885 1110 4 0.45065940535665305 1110 5 0.4677105721579409 1110 6 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0.26664082966234304 2214 9 0.20702896077264654 2214 10 0.019949553808274345 2214 11 0.27183740320199945 2214 12 0.33030315344257133 2214 13 0.4513976868655334 2214 14 0.4640238443467197 2214 15 0.42420123575720226 2214 16 0.3099695023928888 2214 17 0.40664868924736197 2214 18 0.40317443918599594 2214 19 0.5322961269662159 2214 20 0.5372996613617147 2214 21 0.4019957355842223 2214 22 0.41951617480332004 2214 23 0.544917087611644 2214 24 0.013861742327456739 2214 25 0.255566771300451 2215 1 0.2425786934033134 2215 2 0.3291402366194873 2215 3 0.23599642324433034 2215 4 0.39178480485837497 2215 5 0.5011339019800971 2215 6 0.2561097499461553 2215 7 0.0457079621400851 2215 8 0.23376208975022195 2215 9 0.09203500231258488 2215 10 0.032023517505997745 2215 11 -0.024656348900940372 2215 12 -0.1111759547631479 2215 13 0.07756227863478671 2215 14 -0.003959308955906238 2215 15 -0.1869962488406003 2215 16 0.04245856712272911 2215 17 -0.005767214008443489 2215 18 -0.14787284394427047 2215 19 -0.09812820626822116 2215 20 0.06656673720666362 2215 21 0.01897619215557711 2215 22 0.06431754985449345 2215 23 0.1239477229429724 2215 24 -0.005419803378579502 2215 25 -0.008030837277374303 2216 1 -0.14157194853295607 2216 2 0.022570125011821318 2216 3 -1.7983733226886258 2216 4 -1.3282016243982753 2216 5 -0.30159227986118253 2216 6 -0.01646832360994921 2216 7 -0.22531415549753575 2216 8 -0.05496884519377687 2216 9 0.11470917411284631 2216 10 0.08592711174745915 2216 11 0.1183746064325959 2216 12 0.02398664346596746 2216 13 0.03353925516675815 2216 14 -0.11587114591980452 2216 15 0.13417411078409458 2216 16 0.06220633847910051 2216 17 -0.3293224813621123 2216 18 0.13448218140895563 2216 19 -0.13410906015543134 2216 20 -0.1368423271139818 2216 21 -0.12871427456435214 2216 22 -0.07003113802844041 2216 23 -0.07822961257861749 2216 24 -0.19205763500108475 2216 25 -0.17001236635921657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16569.0 3 11.0 4 281.0 5 1668.0 6 2346.0 7 1731.0 8 1314.0 9 1131.0 10 1055.0 11 925.0 12 847.0 13 852.0 14 879.0 15 828.0 16 863.0 17 894.0 18 1085.0 19 1251.0 20 1584.0 21 2027.0 22 2566.0 23 3196.0 24 3862.0 25 4368.0 26 5204.0 27 5955.0 28 6726.0 29 8031.0 30 10345.0 31 13380.0 32 17363.0 33 22811.0 34 31087.0 35 43288.0 36 63711.0 37 110152.0 38 251635.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.955383312301215 20.008641626747238 10.780195388116672 28.255779672834873 2 13.519288870587612 21.17899741234494 41.34336576090941 23.95834795615804 3 18.411202428481047 25.61218479145045 29.318641521665278 26.657971258403222 4 11.98184540549468 18.08089794506568 38.396219506684886 31.541037142754757 5 12.460131101869612 39.17116319798813 35.66456993008627 12.704135770055982 6 30.811516482829248 38.58803480272981 18.08366100952932 12.516787704911625 7 27.4105900302176 32.160628600904815 22.117600856503017 18.311180512374566 8 24.209007459209637 39.4124255754125 19.935583094535783 16.442983870842077 9 26.14977320075979 15.891684858910772 20.240682999951694 37.717858940377745 10 14.913391887491256 30.11612670010393 34.10553771507902 20.8649436973258 11 33.33333333333333 22.99562477796683 25.309132496930488 18.361909391769345 12 23.71724159421645 25.174659561870925 31.947119129974137 19.160979713938488 13 29.161518506111573 22.935425317264322 25.619999532575587 22.283056644048518 14 20.151568739570692 23.82190673100444 27.342826115069467 28.6836984143554 15 21.988142815964352 33.09291763074454 23.123378596302615 21.795560956988496 16 20.826341464934707 28.824104067047152 29.369742465531452 20.97981200248669 17 20.586580993766173 30.19673709648017 28.453104526028284 20.76357738372537 18 20.694963369678007 28.2000504816007 31.106170285034075 19.998815863687213 19 23.21628616078315 27.331608034015716 27.611281026952998 21.84082477824814 20 23.22730119753575 28.340432957629737 28.487826767089736 19.94443907774478 21 21.942747615782583 27.349778719690832 27.29056286230755 23.416910802219036 22 22.51484448156106 29.464601501041564 27.29888863094227 20.7216653864551 23 21.15356150702454 29.203625309860538 28.28919589729847 21.35361728581645 24 22.859021440557413 27.60863231337086 28.974277874983834 20.55806837108789 25 22.01910341711205 28.437758075322932 28.511304673013697 21.03183383455132 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 242.0 2 267.5 3 293.0 4 293.0 5 293.0 6 468.0 7 643.0 8 643.0 9 643.0 10 953.0 11 1263.0 12 1263.0 13 1263.0 14 2097.5 15 2932.0 16 2932.0 17 2932.0 18 5305.0 19 7678.0 20 7678.0 21 7678.0 22 12367.5 23 17057.0 24 17057.0 25 17057.0 26 25029.5 27 33002.0 28 33002.0 29 33002.0 30 43838.5 31 54675.0 32 54675.0 33 54675.0 34 61565.5 35 68456.0 36 68456.0 37 68456.0 38 72613.5 39 76771.0 40 76771.0 41 76771.0 42 79548.5 43 82326.0 44 82326.0 45 82326.0 46 84463.5 47 86601.0 48 86601.0 49 86601.0 50 81190.0 51 75779.0 52 75779.0 53 75779.0 54 64837.5 55 53896.0 56 53896.0 57 53896.0 58 45438.0 59 36980.0 60 36980.0 61 36980.0 62 29915.5 63 22851.0 64 22851.0 65 22851.0 66 17459.5 67 12068.0 68 12068.0 69 12068.0 70 8672.0 71 5276.0 72 5276.0 73 5276.0 74 3627.5 75 1979.0 76 1979.0 77 1979.0 78 1353.0 79 727.0 80 727.0 81 727.0 82 483.5 83 240.0 84 240.0 85 240.0 86 156.5 87 73.0 88 73.0 89 73.0 90 42.0 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1149853308009554 2 0.10906467691147533 3 0.016048088174117084 4 0.0 5 0.004674200439063228 6 0.34526760576547044 7 0.02212454874489928 8 0.009971627603334886 9 0.009971627603334886 10 0.003427746988646367 11 0.00389516703255269 12 1.558066813021076E-4 13 9.348400878126457E-4 14 0.0 15 0.0024929069008337214 16 6.232267252084304E-4 17 0.0 18 4.674200439063228E-4 19 0.0 20 4.674200439063228E-4 21 0.015736474811512868 22 0.0 23 0.0 24 0.0 25 0.007946140746407487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 641821.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.33207015397481 #Duplication Level Percentage of deduplicated Percentage of total 1 91.76929116100968 73.72017135526771 2 6.180812560169048 9.930349363841371 3 0.9974084304488611 2.4037165202095148 4 0.34805340888764275 1.1183940344036878 5 0.18627084878518652 0.7481761446126018 6 0.10650664478044485 0.5133539556220302 7 0.07802256565684539 0.4387399952557166 8 0.05190338452297172 0.33356050613824756 9 0.03664542342927562 0.2649422453168413 >10 0.19944017150092175 3.0425933371234835 >50 0.021560262388091212 1.2130083972496204 >100 0.02136584859971858 3.694393014829336 >500 0.0011654099234266744 0.7427170143663435 >1k 0.0015538798979022326 1.835884115763529 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1903 0.29650011451791075 No Hit CTGTAGGACGTGGAATATGGCAAGA 1893 0.2949420477048897 No Hit CTTTAGGACGTGAAATATGGCGAGG 1645 0.25630199074196697 No Hit GTATCAACGCAGAGTACTTTTTTTT 1555 0.2422793894247773 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1536 0.2393190624800373 No Hit GTCCTACAGTGTGCATTTCTCATTT 1197 0.18650059751862277 No Hit GTACATGGGAAGCAGTGGTATCAAC 1036 0.16141572182898348 No Hit GGTATCAACGCAGAGTACTTTTTTT 1001 0.1559624879834097 No Hit CTGTAGGACCTGGAATATGGCGAGA 977 0.15222312763215912 No Hit CCCATGTACTCTGCGTTGATACCAC 920 0.143342146797939 No Hit CTGAAGGACCTGGAATATGGCGAGA 911 0.14193988666622 No Hit TATCAACGCAGAGTACTTTTTTTTT 848 0.13212406574418725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.558066813021076E-4 0.0 11 0.0 0.0 0.0 1.558066813021076E-4 0.0 12 0.0 0.0 0.0 1.558066813021076E-4 0.0 13 0.0 0.0 0.0 1.558066813021076E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGAC 50 8.534691E-5 15.234466 3 TACAGCG 50 8.534691E-5 15.234466 5 GTCCTAG 75 9.2883784E-7 13.980228 1 GGCGAGG 465 0.0 13.264049 19 GCTCTAT 60 3.9719656E-4 12.709298 1 TCCAACG 75 1.4932279E-5 12.651862 18 GTATTAG 85 3.7880436E-6 12.335496 1 CTTCTAG 70 1.05533334E-4 12.255396 1 CTGATCA 70 1.1009289E-4 12.20001 9 CTACTAG 55 0.0029888707 12.131604 1 TCTAGCC 55 0.0030134975 12.118324 3 GACGTGA 530 0.0 11.819125 7 GTAATAC 65 7.861302E-4 11.718819 3 GTCCTAA 480 0.0 11.716385 1 CCAACGA 65 8.091147E-4 11.678642 19 TAGGACC 360 0.0 11.637438 4 TAAGACC 90 7.255998E-6 11.637438 4 GTATAAG 75 2.0065607E-4 11.438369 1 CTAGACA 75 2.0270857E-4 11.425848 4 AGGACGT 1085 0.0 11.320541 5 >>END_MODULE