FastQCFastQC Report
Thu 26 May 2016
SRR1926893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478036
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6380.13346275175928174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC402.682775E-416.6838361
GTTCTAA350.002116672216.3433511
TCTTAGG456.582201E-414.8269572
TAGGACT456.656097E-414.8051494
GTCTTAG653.2392727E-614.6671091
GTATTTA400.00514983614.3004311
TCTATAC400.005160636314.2959183
GTGCCAA400.00533604614.22409411
ATAGGAC801.9204472E-613.1045923
GTACAGG701.0525364E-412.2575131
GTCCTAG701.0525364E-412.2575131
GTTCAAG701.0525364E-412.2575131
TAGGACC1103.6921847E-812.1133054
TCCAAGA802.7610653E-511.9145182
TGTAGGA2650.011.8695582
GTCCTAA1459.094947E-1111.834841
TAATACT657.882167E-411.7139644
GTAGGAC2450.011.6701363
GTCCAAG855.117106E-511.2160241
TCTACAC855.1335963E-511.2124843
Produced by FastQC (version 0.11.5)