##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926893_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478036 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.71158657506966 31.0 28.0 31.0 16.0 34.0 2 28.094792024031662 31.0 28.0 31.0 16.0 34.0 3 27.687236944497904 31.0 28.0 31.0 16.0 34.0 4 31.567340116643933 35.0 32.0 37.0 18.0 37.0 5 31.72852881372951 35.0 32.0 37.0 18.0 37.0 6 30.69378875231154 35.0 30.0 37.0 11.0 37.0 7 30.47620681287602 35.0 30.0 37.0 11.0 37.0 8 30.607987682936013 35.0 31.0 37.0 11.0 37.0 9 32.87584407868864 37.0 32.0 39.0 17.0 39.0 10 33.03139303316068 37.0 33.0 39.0 17.0 39.0 11 33.19754160774502 37.0 33.0 39.0 17.0 39.0 12 33.08648302638295 37.0 33.0 39.0 17.0 39.0 13 33.168604456568126 38.0 33.0 39.0 17.0 39.0 14 34.19308796827017 39.0 34.0 40.0 17.0 41.0 15 33.7460881607243 39.0 33.0 40.0 10.0 41.0 16 33.8422231798442 39.0 33.0 40.0 11.0 41.0 17 34.05512764729016 39.0 34.0 40.0 16.0 41.0 18 34.089047686785094 39.0 34.0 41.0 15.0 41.0 19 33.43472667330494 39.0 33.0 40.0 10.0 41.0 20 33.72641181835677 39.0 33.0 40.0 10.0 41.0 21 33.72031604314319 39.0 34.0 40.0 8.0 41.0 22 33.741287267067754 39.0 34.0 40.0 2.0 41.0 23 33.71538336024902 39.0 34.0 41.0 2.0 41.0 24 32.391355462768495 38.0 32.0 40.0 2.0 41.0 25 32.96191291032475 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.30778217578576417 1101 2 -0.2039610105838321 1101 3 -1.3437398437653911 1101 4 -0.40877027744090455 1101 5 -0.061171392120609624 1101 6 -2.2418749153408655 1101 7 -0.7903718736068477 1101 8 -0.023398982528455292 1101 9 -0.05518144921613555 1101 10 -8.783017102329524 1101 11 -3.623997091683009 1101 12 -1.0923415588343346 1101 13 -0.4040182432758641 1101 14 -0.2655169111844842 1101 15 -1.7007924767916123 1101 16 0.019826769419516665 1101 17 0.17895641811933416 1101 18 0.3098684136758507 1101 19 -0.47620665645145266 1101 20 0.20581865674336797 1101 21 -0.15888181948141522 1101 22 0.2923437343587807 1101 23 0.45223793800672496 1101 24 0.1142113440169581 1101 25 0.1124667359671534 1102 1 -1.0432000276886306 1102 2 -1.0740167042090079 1102 3 -1.0742021607399685 1102 4 -0.8741301900079037 1102 5 -0.9262565794128825 1102 6 -1.7666001900661392 1102 7 -1.2708978190510507 1102 8 -0.4004163793006228 1102 9 -0.7155551354753769 1102 10 -0.8055078949156851 1102 11 -0.9321535129184468 1102 12 -0.5590039836030272 1102 13 -0.8326083861030682 1102 14 -1.2298812253444424 1102 15 -0.6888714958651789 1102 16 -0.565966864739238 1102 17 -0.9367613110180102 1102 18 -1.268753917671276 1102 19 -0.6385865109294002 1102 20 -0.875135470872884 1102 21 -0.8994273774656634 1102 22 -0.8784949637278743 1102 23 -1.1943845131116433 1102 24 -4.407115421860219 1102 25 -3.047169608614695 1103 1 -0.3730108418744429 1103 2 -0.6357830628503685 1103 3 -0.3109029599407691 1103 4 -0.5080743708270816 1103 5 -0.6689982127795169 1103 6 -0.24613627897722878 1103 7 -0.3445117051649369 1103 8 0.10395425007000725 1103 9 -0.006545395215638905 1103 10 -0.05839563217842425 1103 11 -0.14344222420715624 1103 12 -0.30065295962700134 1103 13 -0.2194559135755938 1103 14 -0.22188921735243383 1103 15 0.15345804958936782 1103 16 0.05634832007594781 1103 17 -0.17503865867035984 1103 18 -0.47022432226668087 1103 19 -2.7547047676726564 1103 20 -0.9187168894543092 1103 21 -0.6242026022408922 1103 22 -0.4929586250665352 1103 23 -0.37688950811663346 1103 24 1.1232604522656544 1103 25 0.1606538179587318 1104 1 -0.16745520030892536 1104 2 0.03718340358510375 1104 3 -1.3260286721032664 1104 4 -0.036835672536273734 1104 5 0.27806566510009745 1104 6 -1.3917386755588481 1104 7 0.07475074219696509 1104 8 0.798126903030127 1104 9 0.7186605126367311 1104 10 0.47583848350154057 1104 11 0.4918067230308907 1104 12 0.6992115746806533 1104 13 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1110 8 1.6962462173619741 1110 9 1.2143390444188 1110 10 1.3426911282833416 1110 11 0.7775534467885095 1110 12 0.9235458739468356 1110 13 0.7503688218741402 1110 14 0.6342315137682988 1110 15 1.3686681866745047 1110 16 1.105380306607934 1110 17 0.4244006751439784 1110 18 0.7051980562807003 1110 19 -1.31512293746583 1110 20 0.3562951355577155 1110 21 0.34874112070283303 1110 22 0.6265925413596278 1110 23 0.6247038059766794 1110 24 -0.16983418457898125 1110 25 0.20389876745367985 1111 1 0.5362505217619216 1111 2 0.39879648260417966 1111 3 0.9336879491501406 1111 4 0.7922324473547384 1111 5 0.8371646660083663 1111 6 1.1101250846591348 1111 7 1.108109506956275 1111 8 1.4693784924942506 1111 9 0.7194924835722389 1111 10 0.4538657314579453 1111 11 0.3749820182170822 1111 12 0.5546993131002722 1111 13 0.5222524197577414 1111 14 0.6000198735566613 1111 15 -0.6432048291985097 1111 16 -0.42121228598465876 1111 17 -0.07262577988247898 1111 18 0.3800446171272611 1111 19 0.6512156868727956 1111 20 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0.8780543303063872 2215 5 0.966111230893862 2215 6 1.6817892067965374 2215 7 1.304791440798514 2215 8 -0.19083524796376494 2215 9 0.36023548611677114 2215 10 -0.08721314894918208 2215 11 -0.015521798576806134 2215 12 -0.39938720089463686 2215 13 0.2496519108518882 2215 14 0.35852369035055176 2215 15 0.42276972550886427 2215 16 0.6101159582901516 2215 17 0.05397981808189911 2215 18 -0.5348459681144746 2215 19 0.5337703942519312 2215 20 -3.367421492739062 2215 21 -1.4930436848224318 2215 22 -0.5797364703128949 2215 23 -0.447138756323902 2215 24 -0.4571741417662665 2215 25 -0.17862879827999478 2216 1 -0.6298254276051694 2216 2 -1.6080895933451878 2216 3 -0.6801348356599846 2216 4 -0.9813878058098204 2216 5 -0.9996117052996532 2216 6 -1.7064318773661817 2216 7 -10.209341969836974 2216 8 -11.009423195269463 2216 9 -6.575410470014258 2216 10 -4.102235534364841 2216 11 -3.039414606255491 2216 12 -2.487933598292134 2216 13 -2.350270786993317 2216 14 -2.453448480873721 2216 15 -3.475896812320876 2216 16 -2.8304612311323396 2216 17 -2.755731265114548 2216 18 -2.427837239412824 2216 19 -1.5876197561651786 2216 20 -2.6000172346787025 2216 21 -5.269311923299579 2216 22 -3.625219125641909 2216 23 -3.994280187057946 2216 24 -3.9445545649955633 2216 25 -3.4157918091989217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29457.0 3 3.0 4 241.0 5 1338.0 6 1693.0 7 1311.0 8 1032.0 9 997.0 10 915.0 11 919.0 12 953.0 13 899.0 14 948.0 15 972.0 16 986.0 17 1133.0 18 1282.0 19 1684.0 20 2110.0 21 2647.0 22 3217.0 23 3957.0 24 4594.0 25 5231.0 26 6053.0 27 6612.0 28 7261.0 29 8636.0 30 10276.0 31 12778.0 32 16146.0 33 20930.0 34 28328.0 35 39780.0 36 56567.0 37 88815.0 38 107335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.58386699842992 17.362440178850303 11.88625020176209 26.167442620957683 2 14.962152153787509 19.4540442947566 41.05307914326048 24.530724408195407 3 19.22932720604407 23.440023291518912 28.69758099143953 28.633068510997497 4 11.889395712398708 16.346349091381047 37.501125176103145 34.2631300201171 5 13.109598528614802 37.39540547835712 36.02320068128984 13.47179531173823 6 32.588021869723235 36.348476003356204 17.70815678741884 13.355345339501717 7 28.50252896615528 30.84772288153194 21.568903051752024 19.080845100560754 8 25.44974366289363 36.290789484724655 20.674154003641583 17.58531284874013 9 26.222857358067365 15.429616393758186 19.317777619691256 39.02974862848319 10 14.600672608671086 29.134377334720124 33.99771053551286 22.26723952109593 11 35.20027283302786 22.48403592022563 23.364675655092185 18.951015591654322 12 23.168935402744243 24.612613140396615 31.24055855682441 20.977892900034732 13 29.16288314677267 21.44764096662831 25.117480908044776 24.271994978554243 14 20.993001286758936 21.456025274874722 27.03156220904079 30.51941122932555 15 23.71650828322391 29.831113454432856 22.654899843918034 23.797478418425204 16 22.898254159552746 27.932819456998427 26.474465200361553 22.694461183087274 17 22.279528226287347 28.258299664602955 26.80163451137274 22.66053759773696 18 22.450533839101332 27.602295623209816 27.859224651796303 22.087945885892545 19 24.20335918880633 26.724320116841348 26.71155628744144 22.36076440691088 20 23.75932896325325 26.938760992327605 27.23691120563577 22.064998838783378 21 23.503726300020507 26.446687338112035 26.61636893927031 23.433217422597153 22 23.454114063094995 27.02522879153712 26.779803576136217 22.74085356923167 23 22.980886922135394 26.987205640816413 26.880289573068033 23.151617863980164 24 23.659846552665663 26.73214087695549 26.99325660269192 22.61475596768693 25 23.097877824807043 26.73024335028758 27.130701135885747 23.041177689019634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 127.0 1 127.0 2 165.0 3 203.0 4 203.0 5 203.0 6 320.0 7 437.0 8 437.0 9 437.0 10 636.5 11 836.0 12 836.0 13 836.0 14 1407.0 15 1978.0 16 1978.0 17 1978.0 18 3443.5 19 4909.0 20 4909.0 21 4909.0 22 7537.5 23 10166.0 24 10166.0 25 10166.0 26 14364.5 27 18563.0 28 18563.0 29 18563.0 30 24170.5 31 29778.0 32 29778.0 33 29778.0 34 35011.5 35 40245.0 36 40245.0 37 40245.0 38 45090.0 39 49935.0 40 49935.0 41 49935.0 42 53667.0 43 57399.0 44 57399.0 45 57399.0 46 60480.5 47 63562.0 48 63562.0 49 63562.0 50 62459.0 51 61356.0 52 61356.0 53 61356.0 54 56511.0 55 51666.0 56 51666.0 57 51666.0 58 44792.0 59 37918.0 60 37918.0 61 37918.0 62 31314.0 63 24710.0 64 24710.0 65 24710.0 66 19263.0 67 13816.0 68 13816.0 69 13816.0 70 10150.0 71 6484.0 72 6484.0 73 6484.0 74 4523.5 75 2563.0 76 2563.0 77 2563.0 78 1755.0 79 947.0 80 947.0 81 947.0 82 617.5 83 288.0 84 288.0 85 288.0 86 199.5 87 111.0 88 111.0 89 111.0 90 70.5 91 30.0 92 30.0 93 30.0 94 18.0 95 6.0 96 6.0 97 6.0 98 4.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2068881841534947 2 0.12509518111606657 3 0.12739626304295074 4 0.06966002560476617 5 0.02489352266356509 6 0.024056765599243572 7 0.2842882126032349 8 0.27612983122610013 9 0.02050054807587713 10 0.04079190688567388 11 0.01840865541507334 12 0.018827033947234097 13 0.017990276882912583 14 0.01903622321331448 15 0.01715351981859107 16 0.02091892660803789 17 0.019872980277635992 18 0.017362709084671447 19 0.025730279727886603 20 0.01861784468115372 21 0.01715351981859107 22 0.018827033947234097 23 0.01903622321331448 24 0.018199466148992965 25 0.017362709084671447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 478036.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.88319400559219 #Duplication Level Percentage of deduplicated Percentage of total 1 93.76763749858593 83.34367115232853 2 4.717975499798664 8.386974633244709 3 0.7924700332454302 2.11311803125715 4 0.2813472569434867 1.000281712873965 5 0.13436313242795025 0.5971312183396289 6 0.08064796483151027 0.4300949222565182 7 0.04874639821712455 0.3032914898864575 8 0.026693822339452226 0.18981057517986746 9 0.022377465072510157 0.1790082512453955 >10 0.11384740892298419 1.7221063543774493 >50 0.008241919165117729 0.5540307558192002 >100 0.005416117097820831 1.0469442336458128 >500 2.3548335207893676E-4 0.13353666954532517 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 638 0.13346275175928174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0918926608037887E-4 2 0.0 0.0 0.0 0.0 2.0918926608037887E-4 3 0.0 0.0 0.0 0.0 2.0918926608037887E-4 4 0.0 0.0 0.0 0.0 2.0918926608037887E-4 5 0.0 0.0 0.0 0.0 2.0918926608037887E-4 6 0.0 0.0 0.0 0.0 2.0918926608037887E-4 7 0.0 0.0 0.0 0.0 2.0918926608037887E-4 8 0.0 0.0 0.0 0.0 2.0918926608037887E-4 9 0.0 0.0 0.0 0.0 2.0918926608037887E-4 10 0.0 0.0 0.0 0.0 2.0918926608037887E-4 11 0.0 0.0 0.0 0.0 2.0918926608037887E-4 12 0.0 0.0 0.0 0.0 2.0918926608037887E-4 13 0.0 0.0 0.0 0.0 2.0918926608037887E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAC 40 2.682775E-4 16.683836 1 GTTCTAA 35 0.0021166722 16.343351 1 TCTTAGG 45 6.582201E-4 14.826957 2 TAGGACT 45 6.656097E-4 14.805149 4 GTCTTAG 65 3.2392727E-6 14.667109 1 GTATTTA 40 0.005149836 14.300431 1 TCTATAC 40 0.0051606363 14.295918 3 GTGCCAA 40 0.005336046 14.224094 11 ATAGGAC 80 1.9204472E-6 13.104592 3 GTACAGG 70 1.0525364E-4 12.257513 1 GTCCTAG 70 1.0525364E-4 12.257513 1 GTTCAAG 70 1.0525364E-4 12.257513 1 TAGGACC 110 3.6921847E-8 12.113305 4 TCCAAGA 80 2.7610653E-5 11.914518 2 TGTAGGA 265 0.0 11.869558 2 GTCCTAA 145 9.094947E-11 11.83484 1 TAATACT 65 7.882167E-4 11.713964 4 GTAGGAC 245 0.0 11.670136 3 GTCCAAG 85 5.117106E-5 11.216024 1 TCTACAC 85 5.1335963E-5 11.212484 3 >>END_MODULE