FastQCFastQC Report
Thu 26 May 2016
SRR1926893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478036
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10330.2160925118610314No Hit
GGTATCAACGCAGAGTACTTTTTTT7190.15040708231179242No Hit
TATCAACGCAGAGTACTTTTTTTTT5550.11610004267461028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1650.014.4312614
TTATACT500.001467956213.3344844
GATATAC751.4147443E-512.7168751
AGTACTG751.4347743E-512.699515
AGGACCT2650.012.5797025
GTAGGAC2900.012.4844873
GTCCTAC2750.011.7920111
TGTAGGA3500.011.7164592
TAGAGCT907.218947E-611.6412174
TAGGACA1406.4574124E-1011.5656254
CTGTAGG3300.011.5607961
GGACCTG2600.011.3562926
GTCCTAG1104.6935747E-711.2717751
CTGTACT1451.1550583E-911.1668114
TAAAGTG1451.1550583E-911.1668115
ACTGGCC855.386368E-511.1597338
GTATTAA600.00569527911.1272661
TTCTAGG600.005699742611.1260962
CTCTACA600.005699742611.1260962
GGACTGA600.00575353511.1120716
Produced by FastQC (version 0.11.5)