##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926893_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478036 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.100996577663608 31.0 30.0 33.0 26.0 34.0 2 30.397649967784854 31.0 30.0 34.0 26.0 34.0 3 30.465161619626972 31.0 30.0 34.0 26.0 34.0 4 34.0995719987616 37.0 35.0 37.0 30.0 37.0 5 34.096147570475864 37.0 35.0 37.0 30.0 37.0 6 34.04933938029772 37.0 35.0 37.0 30.0 37.0 7 34.05833242684651 37.0 35.0 37.0 30.0 37.0 8 34.07922415884996 37.0 35.0 37.0 30.0 37.0 9 35.66769029947535 39.0 35.0 39.0 30.0 39.0 10 35.53974805244793 39.0 35.0 39.0 30.0 39.0 11 35.58143528939243 39.0 35.0 39.0 30.0 39.0 12 35.46687069593085 39.0 35.0 39.0 30.0 39.0 13 35.495222117162726 39.0 35.0 39.0 30.0 39.0 14 36.625628613744574 40.0 37.0 41.0 30.0 41.0 15 36.6004631450351 40.0 37.0 41.0 30.0 41.0 16 36.579211607493995 40.0 37.0 41.0 30.0 41.0 17 36.559177551481476 40.0 37.0 41.0 30.0 41.0 18 36.51851743383344 40.0 37.0 41.0 30.0 41.0 19 36.58790132960698 40.0 37.0 41.0 30.0 41.0 20 36.55238935979717 40.0 37.0 41.0 30.0 41.0 21 36.502662979357204 40.0 37.0 41.0 30.0 41.0 22 36.41601678534671 40.0 37.0 41.0 30.0 41.0 23 36.361506246391485 40.0 37.0 41.0 30.0 41.0 24 36.29474767590725 40.0 37.0 41.0 30.0 41.0 25 36.18141311533022 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15292177261142825 1101 2 0.10562675663108223 1101 3 0.4159236832499431 1101 4 0.3063068039936354 1101 5 0.2781968285057417 1101 6 0.05276318462549057 1101 7 -0.1226714996923235 1101 8 -0.030042121840672564 1101 9 0.2185169356081218 1101 10 0.15092137637427072 1101 11 0.34528816626384895 1101 12 0.24602656378199583 1101 13 0.2094030791128958 1101 14 0.23309106654274814 1101 15 0.08203676659105952 1101 16 0.14880789395748906 1101 17 0.17406619750094876 1101 18 0.09116369986467276 1101 19 0.2165549954572441 1101 20 0.22010542282479406 1101 21 0.20689157376273215 1101 22 -0.0019633799038203392 1101 23 -0.033551749236174544 1101 24 0.0023750251026584124 1101 25 -0.004843712451162219 1102 1 -0.9738523388357088 1102 2 -0.8197595056080402 1102 3 -0.643827192577227 1102 4 -0.9454149075373408 1102 5 -1.0177616408178451 1102 6 -0.6945494482457235 1102 7 -0.6766359276349547 1102 8 -0.9661815954460877 1102 9 -0.7422654699971716 1102 10 -0.8061181780767299 1102 11 -0.9009390397726236 1102 12 -0.8314766550699986 1102 13 -0.6443899262468662 1102 14 -1.0438499760658644 1102 15 -0.8706011949024912 1102 16 -0.9585894770638603 1102 17 -0.7481049555966308 1102 18 -1.1024090761331706 1102 19 -1.0634813850559723 1102 20 -0.9049020628775111 1102 21 -0.9311410339565285 1102 22 -0.8028998412626223 1102 23 -1.0811196445561109 1102 24 -0.8806721048790749 1102 25 -0.6897031309618029 1103 1 -0.4968761899845582 1103 2 -0.3679613038098388 1103 3 -0.18847004972008463 1103 4 -0.2736179544903905 1103 5 -0.21653162083782718 1103 6 -0.3225464512427294 1103 7 -0.09061257598660433 1103 8 0.15810037258588494 1103 9 0.023562452080746255 1103 10 0.023614589156039756 1103 11 -0.1962843194929036 1103 12 -0.2569730336913807 1103 13 -0.18094711905406058 1103 14 -0.2016609151268085 1103 15 -0.11057996613126164 1103 16 -0.1891557857551689 1103 17 -0.2615914690831431 1103 18 -0.27191144876054096 1103 19 -0.21005356287815147 1103 20 -0.10379691802236835 1103 21 -0.5082202047856939 1103 22 -0.3122030380658174 1103 23 -0.29512438981085864 1103 24 -0.4298572946892705 1103 25 -0.5444577513414259 1104 1 -0.48740520286541056 1104 2 -0.23361030058962484 1104 3 -0.1590081968590482 1104 4 -0.35036894532516527 1104 5 -0.2354271496958873 1104 6 -0.21017959466605873 1104 7 -0.35564152694028195 1104 8 -0.4823581981349534 1104 9 -0.40273647038642935 1104 10 -0.39676254733144845 1104 11 -0.20239575744187022 1104 12 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-0.7704192689823159 2216 24 -0.9710288999827057 2216 25 -1.2051920724001093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9294.0 3 16.0 4 146.0 5 1081.0 6 1365.0 7 1038.0 8 862.0 9 734.0 10 599.0 11 602.0 12 569.0 13 508.0 14 578.0 15 551.0 16 562.0 17 591.0 18 645.0 19 791.0 20 1001.0 21 1428.0 22 1706.0 23 2071.0 24 2462.0 25 2973.0 26 3654.0 27 3944.0 28 4664.0 29 5710.0 30 7294.0 31 9545.0 32 12426.0 33 16604.0 34 22811.0 35 31818.0 36 47349.0 37 81525.0 38 198519.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1150390584096 18.339441663699763 10.241052168631803 28.304467109258834 2 14.852042878352881 19.97038972896341 39.491390195942934 25.68617719674078 3 18.873036695769706 23.91261138554305 28.723922863353817 28.490429055333426 4 12.26832288781598 16.575320687144902 36.87567463538311 34.28068178965601 5 13.233223642232678 38.26622511291629 34.874657171726895 13.625894073124146 6 33.075778280153564 37.11458576037515 16.87164018698455 12.937995772486738 7 29.22296661129081 31.440790382177063 20.72179702309466 18.61444598343746 8 26.019770908520318 37.314294680684135 18.798054291542446 17.867880119253098 9 26.483448890525423 15.413599902923588 18.87843271809764 39.22451848845335 10 14.695650172901075 29.329378120953354 33.65828554931917 22.3166861568264 11 35.69270807183143 22.41970845676223 22.943130658901108 18.94445281250523 12 23.199911303009007 24.793319275697023 30.85860360812665 21.148165813167317 13 29.288834220303244 21.505673260367505 25.013806607089066 24.191685912240185 14 21.343162439648896 21.319524052581816 26.796308227832217 30.541005279937078 15 23.603824107777918 29.738504665076775 22.66369607966194 23.99397514748337 16 23.016821014448794 28.066890779506853 26.040670832348393 22.87561737369596 17 22.351501459098184 28.247513257397472 26.632987124373734 22.767998159130606 18 22.392433979315193 27.574513840077653 27.791654115205677 22.24139806540148 19 23.60873157823172 26.930036503603294 26.936312194713775 22.524919723451212 20 23.700186180783632 26.926134343032864 27.295567223814405 22.0781122523691 21 23.528045693244344 26.349770906123815 26.62091763081365 23.50126576981819 22 23.37125237429817 27.0981683387862 26.610338970286758 22.920240316628874 23 22.971008277671206 26.967175906265883 26.878479100815216 23.183336715247695 24 23.09579842480153 26.64135471252105 27.410126873555285 22.852719989122136 25 23.163404490338994 26.607308725449997 27.04810001589945 23.181186768311562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 112.0 2 125.5 3 139.0 4 139.0 5 139.0 6 255.0 7 371.0 8 371.0 9 371.0 10 601.0 11 831.0 12 831.0 13 831.0 14 1302.0 15 1773.0 16 1773.0 17 1773.0 18 3166.0 19 4559.0 20 4559.0 21 4559.0 22 7136.5 23 9714.0 24 9714.0 25 9714.0 26 13800.0 27 17886.0 28 17886.0 29 17886.0 30 23695.0 31 29504.0 32 29504.0 33 29504.0 34 34654.5 35 39805.0 36 39805.0 37 39805.0 38 44662.5 39 49520.0 40 49520.0 41 49520.0 42 53167.0 43 56814.0 44 56814.0 45 56814.0 46 60289.0 47 63764.0 48 63764.0 49 63764.0 50 62972.5 51 62181.0 52 62181.0 53 62181.0 54 57146.5 55 52112.0 56 52112.0 57 52112.0 58 45342.5 59 38573.0 60 38573.0 61 38573.0 62 31956.5 63 25340.0 64 25340.0 65 25340.0 66 19831.0 67 14322.0 68 14322.0 69 14322.0 70 10511.5 71 6701.0 72 6701.0 73 6701.0 74 4666.0 75 2631.0 76 2631.0 77 2631.0 78 1811.5 79 992.0 80 992.0 81 992.0 82 641.0 83 290.0 84 290.0 85 290.0 86 190.0 87 90.0 88 90.0 89 90.0 90 51.0 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11421733927988688 2 0.10438544377410906 3 0.016525952020349934 4 0.0 5 0.00564811018417023 6 0.3416060715092587 7 0.018827033947234097 8 0.01276054523090311 9 0.012969734496983491 10 0.003556217523366441 11 0.005857299450250609 12 8.367570643215155E-4 13 8.367570643215155E-4 14 0.0 15 0.003347028257286062 16 6.275677982411367E-4 17 2.0918926608037887E-4 18 8.367570643215155E-4 19 2.0918926608037887E-4 20 0.0012551355964822734 21 0.013806491561305007 22 0.0 23 6.275677982411367E-4 24 2.0918926608037887E-4 25 0.006694056514572124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 478036.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.83999407930129 #Duplication Level Percentage of deduplicated Percentage of total 1 91.75222100067762 78.76010107460911 2 6.248112800607538 10.726759316219155 3 1.065071261631104 2.7427713237734377 4 0.3723244191201508 1.2784130373141216 5 0.1789022781281708 0.7678485247647848 6 0.09566329694178119 0.4927042105853363 7 0.060134441367311595 0.36133580636524826 8 0.043771791098838976 0.30058962310118004 9 0.030130188333645547 0.23277376693515306 >10 0.13658645134457487 1.9995912182507762 >50 0.009761184429509058 0.6133427665066815 >100 0.006588797687782122 1.2405106308892662 >500 4.8805908798361995E-4 0.2668710813496381 >1k 2.4402954399180998E-4 0.21638761933608805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1033 0.2160925118610314 No Hit GGTATCAACGCAGAGTACTTTTTTT 719 0.15040708231179242 No Hit TATCAACGCAGAGTACTTTTTTTTT 555 0.11610004267461028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 2.0918926608037887E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 165 0.0 14.431261 4 TTATACT 50 0.0014679562 13.334484 4 GATATAC 75 1.4147443E-5 12.716875 1 AGTACTG 75 1.4347743E-5 12.69951 5 AGGACCT 265 0.0 12.579702 5 GTAGGAC 290 0.0 12.484487 3 GTCCTAC 275 0.0 11.792011 1 TGTAGGA 350 0.0 11.716459 2 TAGAGCT 90 7.218947E-6 11.641217 4 TAGGACA 140 6.4574124E-10 11.565625 4 CTGTAGG 330 0.0 11.560796 1 GGACCTG 260 0.0 11.356292 6 GTCCTAG 110 4.6935747E-7 11.271775 1 CTGTACT 145 1.1550583E-9 11.166811 4 TAAAGTG 145 1.1550583E-9 11.166811 5 ACTGGCC 85 5.386368E-5 11.159733 8 GTATTAA 60 0.005695279 11.127266 1 TTCTAGG 60 0.0056997426 11.126096 2 CTCTACA 60 0.0056997426 11.126096 2 GGACTGA 60 0.005753535 11.112071 6 >>END_MODULE