FastQCFastQC Report
Thu 26 May 2016
SRR1926892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586497
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11120.18960028781050883No Hit
GTCCTAAAGTGTGTATTTCTCATTT9790.16692327496986345No Hit
CTGTAGGACGTGGAATATGGCAAGA9280.15822757831668363No Hit
GTCCTACAGTGTGCATTTCTCATTT8880.15140742407889554No Hit
CTTTAGGACGTGAAATATGGCGAGG7820.1333340153487571No Hit
GTATCAACGCAGAGTACTTTTTTTT7820.1333340153487571No Hit
CTGTAGGACCTGGAATATGGCGAGA6090.10383684827032363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC653.2119951E-614.6803951
CTAGAGT653.2930184E-614.6452114
GATCTAG400.00512083314.3133851
CCATAGA500.001448324313.35915851
ATAAGAC1009.600626E-913.3408453
CTAATAC500.001463381613.3408453
TAGGACC2550.013.0658254
AGGACCT4400.012.7602645
GTCCTAC6300.012.7230081
AGTGCCC604.0505172E-412.6794938
TGGCGAG6150.012.64489418
TCCAACG604.1449917E-412.64489418
GTATAAA1457.2759576E-1212.5036461
GTACTAG550.002964291312.144691
GGCGAGG2350.012.106812519
TATGGCG6450.012.0567616
TCCTACA6850.011.964662
GGCGAGA4300.011.90972519
ATGGCGA6850.011.76805817
GTTCAGA657.7174365E-411.7443161
Produced by FastQC (version 0.11.5)