##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926892_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586497 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.81170236164891 31.0 28.0 31.0 16.0 34.0 2 28.21522019720476 31.0 28.0 31.0 16.0 34.0 3 27.779159995703303 31.0 28.0 31.0 16.0 34.0 4 31.63637665665809 35.0 32.0 37.0 19.0 37.0 5 31.8129010037562 35.0 32.0 37.0 19.0 37.0 6 30.80278159990588 35.0 31.0 37.0 11.0 37.0 7 30.603578534928566 35.0 30.0 37.0 11.0 37.0 8 30.73226973027995 35.0 32.0 37.0 11.0 37.0 9 32.96202708624256 37.0 33.0 39.0 17.0 39.0 10 33.123630640906946 37.0 33.0 39.0 17.0 39.0 11 33.28206452888932 37.0 33.0 39.0 17.0 39.0 12 33.165840575484616 37.0 33.0 39.0 17.0 39.0 13 33.27468000688835 38.0 33.0 39.0 17.0 39.0 14 34.26753589532427 39.0 34.0 40.0 17.0 41.0 15 33.82438273341552 39.0 33.0 40.0 11.0 41.0 16 33.92195015490275 39.0 33.0 40.0 16.0 41.0 17 34.14672027307898 39.0 34.0 40.0 17.0 41.0 18 34.19464549690791 39.0 34.0 41.0 17.0 41.0 19 33.568268891400976 39.0 33.0 40.0 10.0 41.0 20 33.86150142285468 39.0 34.0 41.0 10.0 41.0 21 33.85426523920839 39.0 34.0 41.0 10.0 41.0 22 33.86806923138567 39.0 34.0 41.0 8.0 41.0 23 33.847100667181586 39.0 34.0 41.0 2.0 41.0 24 32.53265745604837 38.0 32.0 40.0 2.0 41.0 25 33.12125040707796 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.38451856314017974 1101 2 -0.11859219926589759 1101 3 -0.7678897733595171 1101 4 -0.48394453345464683 1101 5 -0.16499927090804078 1101 6 -2.2492517214894363 1101 7 -0.7032117584582949 1101 8 -0.2157927894737952 1101 9 -0.26920453459160854 1101 10 -9.663769379839845 1101 11 -4.462043321076781 1101 12 -1.8769396349587772 1101 13 -1.1123156513726613 1101 14 -0.6230627440056224 1101 15 -2.01675684037928 1101 16 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2216 11 -2.3922365914848633 2216 12 -1.970380887836491 2216 13 -1.6119049323819432 2216 14 -1.6411868371750487 2216 15 -3.0480644766527405 2216 16 -2.0433425820843176 2216 17 -2.2358151441341043 2216 18 -1.9154122258928297 2216 19 -1.0698473166837772 2216 20 -2.1157065721247967 2216 21 -4.610935399470513 2216 22 -3.1205884210156114 2216 23 -3.0576578630799176 2216 24 -3.1138989982422807 2216 25 -2.676574137528007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35129.0 3 5.0 4 234.0 5 1517.0 6 2056.0 7 1545.0 8 1228.0 9 1159.0 10 1149.0 11 1099.0 12 1139.0 13 1093.0 14 1103.0 15 1162.0 16 1217.0 17 1399.0 18 1485.0 19 1870.0 20 2458.0 21 3152.0 22 3958.0 23 4895.0 24 5547.0 25 6616.0 26 7444.0 27 7931.0 28 9130.0 29 10543.0 30 12518.0 31 15374.0 32 19611.0 33 25812.0 34 35183.0 35 48230.0 36 69739.0 37 108357.0 38 134410.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72022172971735 18.78158016598971 12.57858196190473 25.919616142388207 2 13.852993449188961 20.477046552245152 42.683561851982745 22.98639814658315 3 18.67669003479609 24.92718090210313 29.522211105381956 26.873917957718824 4 11.387329591018444 17.444078554488218 39.29294135712945 31.87565049736389 5 12.213058278588434 38.240249950541994 37.092141975182315 12.454549795687262 6 30.284704670493657 38.13599789890886 18.83205255456527 12.747244876032212 7 26.89794794717772 31.353609345637786 23.11632973057082 18.632112976613676 8 23.718821171852024 37.86769986252933 21.9241791085608 16.489299857057855 9 25.976581818677925 15.504568694127718 20.59885876442319 37.91999072277116 10 13.972416757348652 29.569263412121483 35.644160478118636 20.81415935241123 11 33.62613638107712 22.80401977486396 25.31168944118444 18.25815440287448 12 22.763704949232086 25.23115857256384 32.582700368694084 19.422436109509988 13 28.63389170928525 22.524441382462232 25.914942454250745 22.92672445400177 14 20.06214209461833 22.83517338053677 28.193991111838717 28.90869341300618 15 22.587592896389193 31.824879008741387 23.414664924983715 22.17286316988571 16 21.165177025936973 28.63810853998155 28.966221735623304 21.230492698458175 17 20.95394501232976 29.40179335795875 28.359476522290777 21.28478510742071 18 20.767217196306305 28.286764267016824 30.359052190891962 20.58696634578491 19 23.390005679172784 27.386915390558247 27.418636896204163 21.804442034064802 20 22.867730570230936 28.112497740427084 28.568846202382698 20.450925486959278 21 22.294034187800985 27.341714301305576 27.467565244676063 22.89668626621738 22 22.297560010641273 28.999686218869158 27.670209210158326 21.032544560331242 23 21.39968996584167 28.57754349942956 28.20134143492267 21.821425099806103 24 22.885363428779964 27.506262779224457 28.561683898901947 21.046689893093635 25 22.000190994604402 27.98122113764572 28.45188641278027 21.56670145496961 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 133.0 1 133.0 2 196.5 3 260.0 4 260.0 5 260.0 6 384.5 7 509.0 8 509.0 9 509.0 10 874.5 11 1240.0 12 1240.0 13 1240.0 14 2112.0 15 2984.0 16 2984.0 17 2984.0 18 5194.5 19 7405.0 20 7405.0 21 7405.0 22 11802.0 23 16199.0 24 16199.0 25 16199.0 26 23144.5 27 30090.0 28 30090.0 29 30090.0 30 39377.0 31 48664.0 32 48664.0 33 48664.0 34 55224.0 35 61784.0 36 61784.0 37 61784.0 38 66236.0 39 70688.0 40 70688.0 41 70688.0 42 72995.5 43 75303.0 44 75303.0 45 75303.0 46 76385.5 47 77468.0 48 77468.0 49 77468.0 50 72864.0 51 68260.0 52 68260.0 53 68260.0 54 59569.5 55 50879.0 56 50879.0 57 50879.0 58 42857.0 59 34835.0 60 34835.0 61 34835.0 62 27890.0 63 20945.0 64 20945.0 65 20945.0 66 16017.5 67 11090.0 68 11090.0 69 11090.0 70 7972.0 71 4854.0 72 4854.0 73 4854.0 74 3375.5 75 1897.0 76 1897.0 77 1897.0 78 1300.5 79 704.0 80 704.0 81 704.0 82 456.5 83 209.0 84 209.0 85 209.0 86 139.5 87 70.0 88 70.0 89 70.0 90 45.0 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21824493560921882 2 0.13094696136553127 3 0.13623258089981705 4 0.06939506936949379 5 0.024041043688203007 6 0.023018020552534796 7 0.28440043171576324 8 0.2804788430290351 9 0.01960794343364075 10 0.033930267332995735 11 0.01636837017069141 12 0.017561897162304326 13 0.016027362458802007 14 0.016879881738525518 15 0.015515850890967898 16 0.01943743957769605 17 0.018925928009861943 18 0.0156863547469126 19 0.02455255525603712 20 0.017561897162304326 21 0.015174843179078495 22 0.017220889450414924 23 0.01875542415391724 24 0.01636837017069141 25 0.015856858602857304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 586497.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.11934796805691 #Duplication Level Percentage of deduplicated Percentage of total 1 94.12860838511702 82.0042298765197 2 4.492853651603296 7.828289612871655 3 0.6976826969738166 1.82344984946863 4 0.24244759054252388 0.844875040179645 5 0.11308921899435033 0.4926129510502299 6 0.06455317713510555 0.3374298420766126 7 0.04933643968190408 0.30087109193069966 8 0.030747761728591075 0.21429799626176232 9 0.024050020291171842 0.18856998777468864 >10 0.1313586490684134 2.215294847023315 >50 0.01234237125736615 0.76650540138966 >100 0.011558669981577164 1.9458681719134723 >500 0.0011754579641655909 0.8479144880082776 >1k 1.9590966069426516E-4 0.1897908435316435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1112 0.18960028781050883 No Hit GTCCTAAAGTGTGTATTTCTCATTT 979 0.16692327496986345 No Hit CTGTAGGACGTGGAATATGGCAAGA 928 0.15822757831668363 No Hit GTCCTACAGTGTGCATTTCTCATTT 888 0.15140742407889554 No Hit CTTTAGGACGTGAAATATGGCGAGG 782 0.1333340153487571 No Hit GTATCAACGCAGAGTACTTTTTTTT 782 0.1333340153487571 No Hit CTGTAGGACCTGGAATATGGCGAGA 609 0.10383684827032363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.7050385594470218E-4 0.0 12 0.0 0.0 0.0 1.7050385594470218E-4 1.7050385594470218E-4 13 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAC 65 3.2119951E-6 14.680395 1 CTAGAGT 65 3.2930184E-6 14.645211 4 GATCTAG 40 0.005120833 14.313385 1 CCATAGA 50 0.0014483243 13.3591585 1 ATAAGAC 100 9.600626E-9 13.340845 3 CTAATAC 50 0.0014633816 13.340845 3 TAGGACC 255 0.0 13.065825 4 AGGACCT 440 0.0 12.760264 5 GTCCTAC 630 0.0 12.723008 1 AGTGCCC 60 4.0505172E-4 12.679493 8 TGGCGAG 615 0.0 12.644894 18 TCCAACG 60 4.1449917E-4 12.644894 18 GTATAAA 145 7.2759576E-12 12.503646 1 GTACTAG 55 0.0029642913 12.14469 1 GGCGAGG 235 0.0 12.1068125 19 TATGGCG 645 0.0 12.05676 16 TCCTACA 685 0.0 11.96466 2 GGCGAGA 430 0.0 11.909725 19 ATGGCGA 685 0.0 11.768058 17 GTTCAGA 65 7.7174365E-4 11.744316 1 >>END_MODULE