FastQCFastQC Report
Thu 26 May 2016
SRR1926892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11890.2027290847182509No Hit
GTATCAACGCAGAGTACTTTTTTTT11840.2018765654385274No Hit
CTGTAGGACGTGGAATATGGCAAGA11030.18806575310700652No Hit
GTCCTACAGTGTGCATTTCTCATTT9980.1701628482328128No Hit
CTTTAGGACGTGAAATATGGCGAGG9950.1696513366649787No Hit
GTCCTAAAGTGTGTATTTCTCATTT9600.1636837017069141No Hit
CTGTAGGACCTGGAATATGGCGAGA7880.13435703848442535No Hit
GGTATCAACGCAGAGTACTTTTTTT7820.1333340153487571No Hit
TATCAACGCAGAGTACTTTTTTTTT6310.10758793310110708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3200.015.4690494
CGTCAAG400.005202940714.2791223
TCCAACG655.4985732E-513.13914918
TGTAGGA7600.012.9134622
AGGACCT5050.012.8182225
CTCCTAC603.983716E-412.7045061
ATAGGAC905.253969E-712.69255353
GGACCTG5050.012.44121556
GTAGGAC7500.012.1848523
CTAGACC550.003018008812.1156194
CTGGAAT5550.011.79761410
TCTACAC1052.6376802E-711.7859423
CTAGGAC1052.6376802E-711.7859423
CCCTTAT657.812304E-411.7272371
GTGTAAC907.195229E-611.6457971
CTGTAGG7950.011.6258221
ACCTGGA5400.011.5991278
GGCGAGA4800.011.46634119
TGGCGAG7450.011.46368618
TTACACA1001.8759747E-611.4232994
Produced by FastQC (version 0.11.5)