##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586497 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00418586966344 31.0 30.0 33.0 26.0 34.0 2 30.317542971234296 31.0 30.0 34.0 26.0 34.0 3 30.36893283341603 31.0 30.0 34.0 26.0 34.0 4 33.959537729945765 37.0 35.0 37.0 30.0 37.0 5 33.93211388975562 37.0 35.0 37.0 30.0 37.0 6 33.883990881453784 37.0 35.0 37.0 30.0 37.0 7 33.88324748464186 37.0 35.0 37.0 30.0 37.0 8 33.90733285933261 37.0 35.0 37.0 30.0 37.0 9 35.48485840507283 39.0 35.0 39.0 30.0 39.0 10 35.365055575731844 39.0 35.0 39.0 30.0 39.0 11 35.40761163313709 39.0 35.0 39.0 30.0 39.0 12 35.30326497833749 39.0 35.0 39.0 30.0 39.0 13 35.32894797415844 39.0 35.0 39.0 30.0 39.0 14 36.421238301304186 40.0 37.0 41.0 30.0 41.0 15 36.41662276192376 40.0 37.0 41.0 30.0 41.0 16 36.4017036745286 40.0 37.0 41.0 30.0 41.0 17 36.388486215615764 40.0 37.0 41.0 30.0 41.0 18 36.36540169855941 40.0 37.0 41.0 30.0 41.0 19 36.435889697645514 40.0 37.0 41.0 30.0 41.0 20 36.40136948697095 40.0 37.0 41.0 30.0 41.0 21 36.34814500329925 40.0 37.0 41.0 30.0 41.0 22 36.267871787920484 40.0 37.0 41.0 29.0 41.0 23 36.1944443023579 40.0 37.0 41.0 29.0 41.0 24 36.14086346562728 40.0 37.0 41.0 29.0 41.0 25 36.01981595813789 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05816592782430874 1101 2 -0.1346027492414379 1101 3 0.1509360069899799 1101 4 -0.019025678141666447 1101 5 -0.16354446986880333 1101 6 -0.09542249728834662 1101 7 -0.04876024835279935 1101 8 -0.008325708031456713 1101 9 0.002905419137654519 1101 10 -0.10929861388025586 1101 11 -0.007832816329674586 1101 12 -0.029283925380006792 1101 13 0.020124485420723204 1101 14 0.014726653854665983 1101 15 -0.16704493661125497 1101 16 -0.11518694348819025 1101 17 0.07381178070126282 1101 18 0.16977955200313488 1101 19 0.1745074015612076 1101 20 0.0794648595138483 1101 21 0.23785713745644443 1101 22 0.25886129272318925 1101 23 0.07848738578854864 1101 24 0.2661177487304087 1101 25 0.28263544487840164 1102 1 -0.8349068315264461 1102 2 -0.7392547823507201 1102 3 -0.5257218962938168 1102 4 -0.6653663754788326 1102 5 -0.9110026655920791 1102 6 -0.6865324975690257 1102 7 -0.652146558496355 1102 8 -0.7392065416176195 1102 9 -0.8788824713552543 1102 10 -0.5741504093490448 1102 11 -0.6184298713518714 1102 12 -0.6345641420097579 1102 13 -0.6587788398085976 1102 14 -0.4756427581521905 1102 15 -0.7252367838584135 1102 16 -0.6319921892215561 1102 17 -0.6521411536508808 1102 18 -0.6602727476163466 1102 19 -0.8174023966314934 1102 20 -0.6743633844191734 1102 21 -0.7205651328522578 1102 22 -0.6716602733516979 1102 23 -0.5119797288041568 1102 24 -0.6834538167430182 1102 25 -0.6264383319346791 1103 1 -0.7060945803542644 1103 2 -0.5736941600158723 1103 3 -0.3917462468491948 1103 4 -0.7244560274575562 1103 5 -0.7681242061216622 1103 6 -0.8998461926661108 1103 7 -0.9120659045173625 1103 8 -0.9504890942453059 1103 9 -0.9579143663378531 1103 10 -0.8656097061029513 1103 11 -0.9499048615434731 1103 12 -1.0817872396628232 1103 13 -1.1319887266743436 1103 14 -0.9503075038734821 1103 15 -0.8797013739316668 1103 16 -0.9489057273842647 1103 17 -0.8589523628817091 1103 18 -0.9703210499594306 1103 19 -1.0691650140910909 1103 20 -1.1297149109918934 1103 21 -1.0879549411221916 1103 22 -1.0581909302249741 1103 23 -1.2151422137699726 1103 24 -1.1262222935148003 1103 25 -1.374417158657259 1104 1 -0.37159347283586897 1104 2 -0.3320094590458389 1104 3 -0.1236173164319041 1104 4 -0.10203450850244167 1104 5 -0.19985397163328855 1104 6 -0.2376354841089423 1104 7 -0.17932155031515862 1104 8 -0.19289559168655757 1104 9 -0.3719378406357876 1104 10 -0.2879586635080429 1104 11 -0.2539453941707066 1104 12 -0.2735050388837408 1104 13 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0.33441955067342377 2214 9 0.42747225936994937 2214 10 0.3340464228257787 2214 11 0.33282822573100646 2214 12 0.3280830063196163 2214 13 0.3081215413158418 2214 14 0.47972848796531053 2214 15 0.4354163445984014 2214 16 0.5326364197467086 2214 17 0.4362082249085688 2214 18 0.5814659333742895 2214 19 0.4923409119537041 2214 20 0.5596755202224344 2214 21 0.4984449580089674 2214 22 0.4188350435032788 2214 23 0.47748862917264745 2214 24 0.3519946203569617 2214 25 0.41560370285439774 2215 1 0.41158554314930385 2215 2 0.3106366078664138 2215 3 0.27106639982542546 2215 4 0.3615531222113475 2215 5 0.2973100638557753 2215 6 0.19483082628354254 2215 7 0.08180415661237816 2215 8 0.019618971374441685 2215 9 0.1095511527559978 2215 10 0.22784019408761935 2215 11 -0.12611119845259822 2215 12 0.04348857931171324 2215 13 -0.03268484796117832 2215 14 -0.05384239758915754 2215 15 -0.10361944537599044 2215 16 -0.22562835896023614 2215 17 -0.08748824620929696 2215 18 -0.201460460829324 2215 19 0.009491601888598211 2215 20 -0.007614897985561697 2215 21 -0.31501036504365487 2215 22 -0.19766287331380283 2215 23 -0.5473747216727247 2215 24 -0.18523935442399875 2215 25 -0.2938910244206454 2216 1 -0.4821638390589307 2216 2 -0.09614299910641932 2216 3 -2.0357271307123526 2216 4 -1.6342028476499806 2216 5 -0.7047242646404541 2216 6 -0.3856404704467593 2216 7 -0.36855833436653285 2216 8 -0.269657439024229 2216 9 -0.33164516844682623 2216 10 -0.40141459758502407 2216 11 -0.19074523721113223 2216 12 -0.320207691404903 2216 13 -0.6828547473395119 2216 14 -0.6563607559689686 2216 15 -0.5096561079293593 2216 16 -0.591988175764385 2216 17 -0.6583958606987323 2216 18 -0.5317284841401388 2216 19 -0.5627158996180128 2216 20 -0.7362552459258822 2216 21 -0.3209698634126852 2216 22 -0.397658409270079 2216 23 -0.6058813289888718 2216 24 -0.5311527881132889 2216 25 -0.6617845591036087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13074.0 3 16.0 4 188.0 5 1391.0 6 1896.0 7 1385.0 8 1114.0 9 907.0 10 798.0 11 761.0 12 701.0 13 667.0 14 644.0 15 661.0 16 736.0 17 750.0 18 832.0 19 995.0 20 1309.0 21 1645.0 22 2092.0 23 2683.0 24 3104.0 25 3656.0 26 4391.0 27 5049.0 28 5806.0 29 7108.0 30 9052.0 31 11859.0 32 15693.0 33 20645.0 34 27844.0 35 39433.0 36 57634.0 37 100151.0 38 239827.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39257165389007 19.676121515028925 10.63947821663705 28.291828614443947 2 13.854709126380632 21.13256627289532 40.72913412975167 24.283590470972378 3 18.20200270119098 25.267049562761763 29.59731108716116 26.9336366488861 4 11.88036767451496 17.880227861353085 38.50335125328859 31.736053210843362 5 12.41990212628097 39.06695994680035 35.817688884341905 12.695449042576772 6 30.992959373101897 38.70171866578837 18.10938208447041 12.19593987663932 7 27.667321558916424 32.034804794357015 22.191296754872468 18.106576891854097 8 24.37137429702714 38.739986153694 20.134950327229816 16.753689222049047 9 26.212400245549418 15.591364845508494 20.106745788145417 38.08948912079667 10 14.302594829438304 30.099885075894246 34.729757840927306 20.86776225374015 11 33.92546264009644 22.838298909920525 24.821176153637605 18.41506229634543 12 23.01259011205609 25.33470192261787 32.19651759955805 19.456190365767988 13 28.95572318121986 22.509769954236376 25.730956261978 22.803550602565764 14 20.3145114126756 23.118447323686226 27.747115501017056 28.819925762621125 15 22.29144820413139 32.06629325558198 23.30767197797045 22.334586562316172 16 21.313602526198054 28.724590532895476 28.62672081896831 21.33508612193816 17 21.01122599301615 29.48391804888695 28.130285628546485 21.374570329550416 18 20.7421060438914 28.400850479033853 30.245373772576993 20.61166970449775 19 22.98664784304097 27.388716395821294 27.72443848817641 21.90019727296133 20 23.013954154532374 28.148210035260497 28.320079387272116 20.517756422935012 21 22.276940706232224 27.34160249370775 27.366157602875674 23.015299197184348 22 22.51452266593009 28.953430281825828 27.409176858534657 21.12287019370943 23 21.63530805889223 28.631957646697753 27.952497463746496 21.78023683066352 24 22.461836974443177 27.57286056024157 28.77201417910066 21.193288286214592 25 22.12144928870678 28.027493448054198 28.32606097188759 21.52499629135143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 183.5 3 183.0 4 183.0 5 183.0 6 320.0 7 457.0 8 457.0 9 457.0 10 751.0 11 1045.0 12 1045.0 13 1045.0 14 1870.0 15 2695.0 16 2695.0 17 2695.0 18 4831.0 19 6967.0 20 6967.0 21 6967.0 22 11109.0 23 15251.0 24 15251.0 25 15251.0 26 22211.0 27 29171.0 28 29171.0 29 29171.0 30 38142.5 31 47114.0 32 47114.0 33 47114.0 34 53799.5 35 60485.0 36 60485.0 37 60485.0 38 65280.5 39 70076.0 40 70076.0 41 70076.0 42 72755.5 43 75435.0 44 75435.0 45 75435.0 46 77040.0 47 78645.0 48 78645.0 49 78645.0 50 74641.0 51 70637.0 52 70637.0 53 70637.0 54 61060.5 55 51484.0 56 51484.0 57 51484.0 58 43675.0 59 35866.0 60 35866.0 61 35866.0 62 28683.0 63 21500.0 64 21500.0 65 21500.0 66 16484.5 67 11469.0 68 11469.0 69 11469.0 70 8221.5 71 4974.0 72 4974.0 73 4974.0 74 3446.0 75 1918.0 76 1918.0 77 1918.0 78 1279.5 79 641.0 80 641.0 81 641.0 82 431.5 83 222.0 84 222.0 85 222.0 86 142.0 87 62.0 88 62.0 89 62.0 90 38.5 91 15.0 92 15.0 93 15.0 94 7.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11253254492350345 2 0.10639440610949416 3 0.015174843179078495 4 0.0 5 0.0046036041105069585 6 0.34646383527963487 7 0.024041043688203007 8 0.01005972750073743 9 0.009718719788848026 10 0.00392158868672815 11 0.004433100254562256 12 8.52519279723511E-4 13 8.52519279723511E-4 14 0.0 15 0.002046046271336426 16 5.115115678341065E-4 17 1.7050385594470218E-4 18 6.820154237788087E-4 19 0.0 20 8.52519279723511E-4 21 0.010400735212626834 22 0.0 23 3.4100771188940436E-4 24 0.0 25 0.0051151156783410655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 586497.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.54717070865418 #Duplication Level Percentage of deduplicated Percentage of total 1 92.55294736996764 78.25089840877739 2 5.8092720842726315 9.823150372040349 3 0.8422986309540312 2.136418984168085 4 0.27426171121719456 0.9275220686851102 5 0.1513730992431766 0.6399083631205451 6 0.08123947677093549 0.4121140746500417 7 0.04962109307986552 0.29367261191613864 8 0.04192666186691954 0.2835824510485174 9 0.01911762391182421 0.14547069111751657 >10 0.1470986836516286 2.413286700320935 >50 0.015522617611834558 0.90111904684012 >100 0.0133050333139213 2.2140134799944264 >500 0.0014111398968885372 0.9663938746238252 >1k 6.047742415236588E-4 0.5924488726970751 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1189 0.2027290847182509 No Hit GTATCAACGCAGAGTACTTTTTTTT 1184 0.2018765654385274 No Hit CTGTAGGACGTGGAATATGGCAAGA 1103 0.18806575310700652 No Hit GTCCTACAGTGTGCATTTCTCATTT 998 0.1701628482328128 No Hit CTTTAGGACGTGAAATATGGCGAGG 995 0.1696513366649787 No Hit GTCCTAAAGTGTGTATTTCTCATTT 960 0.1636837017069141 No Hit CTGTAGGACCTGGAATATGGCGAGA 788 0.13435703848442535 No Hit GGTATCAACGCAGAGTACTTTTTTT 782 0.1333340153487571 No Hit TATCAACGCAGAGTACTTTTTTTTT 631 0.10758793310110708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.4100771188940436E-4 0.0 5 0.0 0.0 0.0 3.4100771188940436E-4 0.0 6 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 7 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 8 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 9 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 10 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 11 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 12 0.0 0.0 0.0 3.4100771188940436E-4 1.7050385594470218E-4 13 0.0 0.0 0.0 3.4100771188940436E-4 3.4100771188940436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 320 0.0 15.469049 4 CGTCAAG 40 0.0052029407 14.279122 3 TCCAACG 65 5.4985732E-5 13.139149 18 TGTAGGA 760 0.0 12.913462 2 AGGACCT 505 0.0 12.818222 5 CTCCTAC 60 3.983716E-4 12.704506 1 ATAGGAC 90 5.253969E-7 12.6925535 3 GGACCTG 505 0.0 12.4412155 6 GTAGGAC 750 0.0 12.184852 3 CTAGACC 55 0.0030180088 12.115619 4 CTGGAAT 555 0.0 11.797614 10 TCTACAC 105 2.6376802E-7 11.785942 3 CTAGGAC 105 2.6376802E-7 11.785942 3 CCCTTAT 65 7.812304E-4 11.727237 1 GTGTAAC 90 7.195229E-6 11.645797 1 CTGTAGG 795 0.0 11.625822 1 ACCTGGA 540 0.0 11.599127 8 GGCGAGA 480 0.0 11.466341 19 TGGCGAG 745 0.0 11.463686 18 TTACACA 100 1.8759747E-6 11.423299 4 >>END_MODULE