FastQCFastQC Report
Thu 26 May 2016
SRR1926891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572689
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8910.15558182538864898No Hit
GTCCTACAGTGTGCATTTCTCATTT7590.1325326660718121No Hit
GTATCAACGCAGAGTACTTTTTTTT7050.1231034645331061No Hit
GTCCTAAAGTGTGTATTTCTCATTT6920.1208334715700843No Hit
CTGTAGGACGTGGAATATGGCAAGA6750.117865019233825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG853.7729897E-612.33929351
GTCCTAC5800.012.3296221
CCTAATA701.057973E-412.2516362
GGTGTGC701.07544445E-412.2301688
ATAGTAC550.002995062912.1278833
ATTATAG802.7709471E-511.9113133
GATTAGA657.767008E-411.7352711
CTCTAGC657.767008E-411.7352711
TGTGCGT658.111272E-411.6747810
TCTATAC907.1887225E-611.6466173
GTCTAGG752.0001017E-411.4418911
GTCTTAC752.0001017E-411.4418911
TCCTACA6750.011.2936892
AATACTG1104.814756E-711.2507445
TGAACTA600.00577742111.10650355
TAATACT951.318453E-511.027834
CCTACAG6050.011.0253483
TAGACAG951.3248804E-511.0229955
CTAAATT3800.010.97967319
GTCCTAA2000.010.9651441
Produced by FastQC (version 0.11.5)