##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926891_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 572689 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.27817366843086 31.0 28.0 31.0 12.0 34.0 2 27.652319147041414 31.0 28.0 31.0 16.0 34.0 3 27.239931271597673 31.0 27.0 31.0 10.0 34.0 4 31.042293461197964 35.0 32.0 37.0 16.0 37.0 5 31.232937947123133 35.0 32.0 37.0 16.0 37.0 6 30.159805758448304 35.0 30.0 37.0 10.0 37.0 7 29.945520168887477 35.0 30.0 37.0 10.0 37.0 8 30.09683266135721 35.0 30.0 37.0 10.0 37.0 9 32.32450597095457 37.0 32.0 39.0 11.0 39.0 10 32.48330420175697 37.0 32.0 39.0 10.0 39.0 11 32.63337867498765 37.0 33.0 39.0 10.0 39.0 12 32.51721615047609 37.0 32.0 39.0 10.0 39.0 13 32.615265877291165 37.0 33.0 39.0 10.0 39.0 14 33.590666138165744 39.0 33.0 40.0 10.0 41.0 15 33.146728852832865 38.0 32.0 40.0 8.0 41.0 16 33.228118577447795 38.0 32.0 40.0 8.0 41.0 17 33.44324406440494 38.0 33.0 40.0 2.0 41.0 18 33.486040416351635 39.0 33.0 40.0 2.0 41.0 19 32.83961277412348 38.0 32.0 40.0 2.0 41.0 20 33.12709690599959 39.0 32.0 40.0 2.0 41.0 21 33.11695527590018 39.0 33.0 40.0 2.0 41.0 22 33.12668481496938 39.0 33.0 40.0 2.0 41.0 23 33.10115088643225 39.0 33.0 40.0 2.0 41.0 24 31.791663538150726 38.0 31.0 40.0 2.0 41.0 25 32.359989453263466 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4398558585401666 1101 2 -0.6986834565840212 1101 3 -1.6481235408430486 1101 4 -0.7096300003445641 1101 5 -0.5982486262533797 1101 6 -2.7673862706578376 1101 7 -1.2736743874441778 1101 8 -0.5805741011192431 1101 9 -0.6159694014442465 1101 10 -9.67609273557542 1101 11 -4.26131269875324 1101 12 -2.0730411446868615 1101 13 -1.1814491466860275 1101 14 -0.8480031182170791 1101 15 -2.133933578406033 1101 16 -0.8115994309040317 1101 17 -0.5623951127155351 1101 18 -0.4768711289540093 1101 19 -1.1073281095239125 1101 20 -0.2459920175890602 1101 21 -0.42592770410153946 1101 22 -0.16074660352946069 1101 23 -0.24945217793459307 1101 24 -0.3994987429555188 1101 25 -0.16032920516692428 1102 1 -0.07509098574752215 1102 2 -0.12731828454625216 1102 3 -0.4555063294582098 1102 4 -0.20837469240667872 1102 5 0.23036450502844374 1102 6 -1.021103588271881 1102 7 -0.4348412743650236 1102 8 0.28570390380055244 1102 9 0.36898380980534284 1102 10 0.3914304333797318 1102 11 0.0032445058126455706 1102 12 0.060758338537659995 1102 13 0.07634263844357747 1102 14 0.006281130838267757 1102 15 0.4575165206379026 1102 16 0.33891917611273215 1102 17 0.21880513637362498 1102 18 -0.12137102047061177 1102 19 0.6503213791952263 1102 20 0.30701550862305993 1102 21 0.17776833949470472 1102 22 -0.051593985698168865 1102 23 -0.48980772171820774 1102 24 -3.823151787676043 1102 25 -2.189333722021299 1103 1 0.11478508151187938 1103 2 0.010297812618986768 1103 3 0.35621139556405623 1103 4 0.18660807685187208 1103 5 0.014585551021319532 1103 6 0.7299145919046275 1103 7 -0.3108456029133322 1103 8 0.6191731312969573 1103 9 0.47935495135735096 1103 10 0.4505717555569717 1103 11 0.028503035337472227 1103 12 -0.0498399013137103 1103 13 -0.08633635537415074 1103 14 -0.05960747334170691 1103 15 0.26595773706394965 1103 16 0.23645406511501932 1103 17 -0.1314803097719306 1103 18 -0.3534367531157159 1103 19 -2.27325730694535 1103 20 -0.6421637225534269 1103 21 -0.7162246368747631 1103 22 -0.5669128604397784 1103 23 -0.32458139637390104 1103 24 1.1721694401322829 1103 25 0.6402153236870234 1104 1 -0.5917524597953587 1104 2 -0.4303444881406371 1104 3 -2.002185239773482 1104 4 -0.719963797745784 1104 5 -0.3260369008047803 1104 6 -2.0408382727792933 1104 7 -0.6715932639579236 1104 8 -0.3088181566306005 1104 9 -0.38273385384414027 1104 10 0.025809942759238425 1104 11 -0.19455874312696864 1104 12 -0.17315274196432995 1104 13 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1110 8 1.833580541059252 1110 9 1.3994648832468144 1110 10 1.5582691937235325 1110 11 1.1071577970414168 1110 12 1.1413484193313437 1110 13 1.2111389222778328 1110 14 1.1739990893533374 1110 15 1.7861488981351883 1110 16 1.606580152009478 1110 17 1.0984501782908822 1110 18 1.3703321252079022 1110 19 -0.7337930302678544 1110 20 0.8619905142750497 1110 21 0.7591140018746714 1110 22 1.0337133568625632 1110 23 0.84859895965905 1110 24 -0.11442683656078145 1110 25 0.39769301485782194 1111 1 0.3356057738544962 1111 2 -0.10864560418454161 1111 3 0.6549035915661818 1111 4 0.28919654878276546 1111 5 0.37605465384642756 1111 6 0.7206381731449909 1111 7 0.7911885730955568 1111 8 0.9190510833085632 1111 9 0.2705873016620899 1111 10 0.24770845059004643 1111 11 0.1088978072080451 1111 12 0.2780100606829379 1111 13 -0.09327212778102023 1111 14 -0.18201941938337995 1111 15 -0.9577874771502053 1111 16 -0.595811616250991 1111 17 -0.2996467477325808 1111 18 -0.19522835070974054 1111 19 0.4743631431017832 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0.562829468291369 2213 20 -1.4706677248418387 2213 21 -0.2360154803370449 2213 22 -0.44650118956315055 2213 23 -0.15165756833413013 2213 24 -0.11421599163488239 2213 25 0.03668550409302185 2214 1 0.3171430428501445 2214 2 0.011752072705920824 2214 3 0.04649200890376548 2214 4 0.059831175799189396 2214 5 0.3432421997243189 2214 6 0.3179580235170043 2214 7 0.6169318683172094 2214 8 -2.7600086328210764 2214 9 -1.4574362347965213 2214 10 -1.203951962002666 2214 11 -0.5698894309576588 2214 12 -1.865150096461683 2214 13 -0.9436582361027561 2214 14 -0.3858129703467412 2214 15 -2.0909552096520088 2214 16 -0.5180837536174678 2214 17 -0.3528245807106387 2214 18 -0.236233935333523 2214 19 0.6805631912552172 2214 20 -1.625119696239416 2214 21 -0.678088934544391 2214 22 -0.566579337047159 2214 23 -0.777515973062826 2214 24 -2.1599045971561246 2214 25 -1.4412992648959353 2215 1 0.6809937435209292 2215 2 0.04639598140840917 2215 3 0.5289243216708073 2215 4 0.7850774621333407 2215 5 0.8349880100893792 2215 6 1.5588537309399086 2215 7 1.0680288110459522 2215 8 -0.3839188849740047 2215 9 0.23190759199500377 2215 10 0.08084248576249564 2215 11 -0.1801371562681453 2215 12 -0.4048957297219644 2215 13 -0.13016395880094933 2215 14 0.0774145428807742 2215 15 0.29529371111573965 2215 16 0.26042900899570753 2215 17 -0.2811491694954782 2215 18 -0.9075064331750582 2215 19 0.07747381975580225 2215 20 -3.844098687184932 2215 21 -2.1471033464458316 2215 22 -1.050057674918797 2215 23 -0.8043772336060968 2215 24 -0.787856158998899 2215 25 -0.39532286410781836 2216 1 -1.385392952188493 2216 2 -1.8429816170858473 2216 3 -1.2203400365977615 2216 4 -1.703951976790723 2216 5 -1.8428749175438028 2216 6 -2.9978149828579035 2216 7 -10.256592432873408 2216 8 -11.23710534032961 2216 9 -6.885964083810116 2216 10 -4.488724258812976 2216 11 -3.8584363879312242 2216 12 -3.2151542868037843 2216 13 -3.1648926550140466 2216 14 -3.2987734191044424 2216 15 -4.253909233218494 2216 16 -3.27477796479954 2216 17 -3.495663844774821 2216 18 -3.226391659810151 2216 19 -2.7082346275320397 2216 20 -3.723486580245158 2216 21 -6.614707496083753 2216 22 -4.8499056864769905 2216 23 -4.4964714045842555 2216 24 -4.727308205320028 2216 25 -4.1266302739861835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41073.0 3 3.0 4 315.0 5 1849.0 6 2218.0 7 1676.0 8 1332.0 9 1313.0 10 1254.0 11 1187.0 12 1238.0 13 1185.0 14 1192.0 15 1199.0 16 1366.0 17 1516.0 18 1737.0 19 1992.0 20 2680.0 21 3359.0 22 4238.0 23 5084.0 24 5914.0 25 6681.0 26 7632.0 27 8272.0 28 9226.0 29 10874.0 30 12865.0 31 15390.0 32 19844.0 33 25621.0 34 34711.0 35 47683.0 36 67995.0 37 103519.0 38 117456.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18652373660031 18.422927149420257 12.567228177641654 25.82332093633778 2 14.176207694836304 20.164324830919224 42.15181949796823 23.507647976276235 3 18.902780206329776 24.23168386081483 29.055953838083582 27.809582094771812 4 11.487747427868486 17.136826213375084 38.811227913218524 32.56419844553791 5 12.42343530315865 37.84562181799129 36.951209927430554 12.779732951419515 6 31.045624354878992 37.54075942204144 18.456006022002665 12.957610201076905 7 27.475947686546643 31.122896681455064 22.4661656242392 18.934990007759094 8 24.23011719871943 37.48765315343715 21.529572886675215 16.752656761168204 9 25.63851339550805 15.8402319326557 20.322051067099796 38.199203604736454 10 14.003657776093098 29.666066353525633 35.15203177758485 21.178244092796415 11 34.109036166671615 22.942408212378997 24.35596289899214 18.592592721957253 12 22.637314657957702 25.14975811662795 32.211006130040694 20.001921095373653 13 28.70228554363525 22.231614096426995 25.638186584328647 23.427913775609106 14 20.048760552852457 22.592971634351738 27.968857515097955 29.389410297697843 15 22.877575969263013 31.293049249039466 23.211316800558855 22.618057981138666 16 21.46176698144018 28.665114630069805 28.38358241017428 21.489535978315732 17 21.26452640513607 29.05508070516157 28.107960836776826 21.572432052925542 18 21.13639538945162 28.413552217953196 29.616835487251137 20.833216905344045 19 23.43534408981511 27.212455069740148 27.43287096064301 21.91932987980173 20 22.886922382372 28.086638059760144 28.30983480297386 20.716604754894 21 22.62386067736758 27.233893178297553 27.319991826740882 22.822254317593988 22 22.21969957613724 28.489969279992177 27.802916916263094 21.48741422760749 23 21.897049166585163 28.133863328350078 27.83120031296718 22.13788719209758 24 22.896891858615106 27.54120291376248 28.298285169396063 21.263620058226348 25 22.205184807438403 27.859607821221243 28.141481952371304 21.793725418969046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 121.0 1 121.0 2 185.0 3 249.0 4 249.0 5 249.0 6 361.0 7 473.0 8 473.0 9 473.0 10 803.5 11 1134.0 12 1134.0 13 1134.0 14 1966.5 15 2799.0 16 2799.0 17 2799.0 18 4967.0 19 7135.0 20 7135.0 21 7135.0 22 10971.5 23 14808.0 24 14808.0 25 14808.0 26 21024.0 27 27240.0 28 27240.0 29 27240.0 30 35657.5 31 44075.0 32 44075.0 33 44075.0 34 50650.0 35 57225.0 36 57225.0 37 57225.0 38 62081.5 39 66938.0 40 66938.0 41 66938.0 42 70402.0 43 73866.0 44 73866.0 45 73866.0 46 74939.0 47 76012.0 48 76012.0 49 76012.0 50 72596.0 51 69180.0 52 69180.0 53 69180.0 54 60904.0 55 52628.0 56 52628.0 57 52628.0 58 44350.5 59 36073.0 60 36073.0 61 36073.0 62 29129.5 63 22186.0 64 22186.0 65 22186.0 66 17055.5 67 11925.0 68 11925.0 69 11925.0 70 8672.0 71 5419.0 72 5419.0 73 5419.0 74 3752.5 75 2086.0 76 2086.0 77 2086.0 78 1444.5 79 803.0 80 803.0 81 803.0 82 520.0 83 237.0 84 237.0 85 237.0 86 145.0 87 53.0 88 53.0 89 53.0 90 33.5 91 14.0 92 14.0 93 14.0 94 10.5 95 7.0 96 7.0 97 7.0 98 5.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22944390410851262 2 0.1349772738781433 3 0.1377711113710932 4 0.0700205521670575 5 0.023398389003455627 6 0.021303010883743183 7 0.30487751641816063 8 0.295622929722764 9 0.01903301792072137 10 0.03666911709496777 11 0.01571533589784333 12 0.017286869487627664 13 0.01571533589784333 14 0.016588410114390183 15 0.015540721054533961 16 0.018858403077412 17 0.01938224760734011 18 0.015540721054533961 19 0.023573003846764997 20 0.017461484330937034 21 0.01571533589784333 22 0.017112254644318294 23 0.018334558547483885 24 0.016763024957699553 25 0.016239180427771442 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 572689.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.78867527785376 #Duplication Level Percentage of deduplicated Percentage of total 1 94.64297556226495 84.03224424527791 2 4.120714523649056 7.317455675060236 3 0.627863957480323 1.6724162711816573 4 0.2133285458796639 0.7576463595042483 5 0.11013545148868635 0.48893904194043936 6 0.061442969070618116 0.3273263897350994 7 0.04313763603618659 0.268109349078985 8 0.030038251786088398 0.21336452669995307 9 0.018004648639584946 0.14387480113967632 >10 0.11165997068737704 1.8458644864489024 >50 0.010250706160799851 0.6505789307231984 >100 0.009067881800183322 1.4501005187595042 >500 0.0013798950564422614 0.8320794044502601 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 891 0.15558182538864898 No Hit GTCCTACAGTGTGCATTTCTCATTT 759 0.1325326660718121 No Hit GTATCAACGCAGAGTACTTTTTTTT 705 0.1231034645331061 No Hit GTCCTAAAGTGTGTATTTCTCATTT 692 0.1208334715700843 No Hit CTGTAGGACGTGGAATATGGCAAGA 675 0.117865019233825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 85 3.7729897E-6 12.3392935 1 GTCCTAC 580 0.0 12.329622 1 CCTAATA 70 1.057973E-4 12.251636 2 GGTGTGC 70 1.07544445E-4 12.230168 8 ATAGTAC 55 0.0029950629 12.127883 3 ATTATAG 80 2.7709471E-5 11.911313 3 GATTAGA 65 7.767008E-4 11.735271 1 CTCTAGC 65 7.767008E-4 11.735271 1 TGTGCGT 65 8.111272E-4 11.67478 10 TCTATAC 90 7.1887225E-6 11.646617 3 GTCTAGG 75 2.0001017E-4 11.441891 1 GTCTTAC 75 2.0001017E-4 11.441891 1 TCCTACA 675 0.0 11.293689 2 AATACTG 110 4.814756E-7 11.250744 5 TGAACTA 60 0.005777421 11.1065035 5 TAATACT 95 1.318453E-5 11.02783 4 CCTACAG 605 0.0 11.025348 3 TAGACAG 95 1.3248804E-5 11.022995 5 CTAAATT 380 0.0 10.979673 19 GTCCTAA 200 0.0 10.965144 1 >>END_MODULE