FastQCFastQC Report
Thu 26 May 2016
SRR1926891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572689
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11900.20779166353815073No Hit
GTCCTACAGTGGACATTTCTAAATT9570.16710640504706745No Hit
CTGTAGGACGTGGAATATGGCAAGA9220.16099488553123947No Hit
GTCCTACAGTGTGCATTTCTCATTT8340.14562877932001486No Hit
GGTATCAACGCAGAGTACTTTTTTT8020.140041104334115No Hit
GTCCTAAAGTGTGTATTTCTCATTT6800.11873809345037184No Hit
CTGTAGGACCTGGAATATGGCGAGA6320.11035658097152205No Hit
CTTTAGGACGTGAAATATGGCGAGG6310.1101819661282127No Hit
TATCAACGCAGAGTACTTTTTTTTT6310.1101819661282127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG551.9783936E-413.80027418
GTCTTAG852.57618E-713.4590181
CTAATAC604.0038742E-412.6968133
GTCCAGA701.0534027E-412.2573191
TGTAGGA6150.012.2451722
ATAAGAC701.0646778E-412.2433563
GTAGGAC5850.012.2084733
ACTGTTC1251.4279067E-912.1453028
GTCTAGG550.00298467912.1335081
GTGCTAC550.00298467912.1335081
GTATTAT959.928426E-712.0422791
GACAGGT951.0511994E-611.98549657
CTGTAGG6150.011.9362141
GTCCTAC4750.011.8415741
TCTTAGG907.1587E-611.6509982
AGGACTG1156.809387E-811.5927425
TAGGACC2450.011.271664
AGGACGT4750.011.2266555
TAGGACG5300.011.1396574
TAACCTG600.0057650211.1097115
Produced by FastQC (version 0.11.5)