##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 572689 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.945762883519677 31.0 30.0 33.0 26.0 34.0 2 30.26170050411305 31.0 30.0 34.0 26.0 34.0 3 30.308045029675792 31.0 30.0 34.0 26.0 34.0 4 33.882354995468745 37.0 35.0 37.0 30.0 37.0 5 33.881495890439666 37.0 35.0 37.0 30.0 37.0 6 33.8246238359738 37.0 35.0 37.0 30.0 37.0 7 33.81883710006653 37.0 35.0 37.0 29.0 37.0 8 33.83857556195422 37.0 35.0 37.0 30.0 37.0 9 35.40000419075624 39.0 35.0 39.0 30.0 39.0 10 35.29485113211533 39.0 35.0 39.0 30.0 39.0 11 35.31800506033816 39.0 35.0 39.0 30.0 39.0 12 35.21675813574209 39.0 35.0 39.0 29.0 39.0 13 35.233837213566176 39.0 35.0 39.0 30.0 39.0 14 36.34308673643112 40.0 36.0 41.0 29.0 41.0 15 36.330233337815116 40.0 36.0 41.0 29.0 41.0 16 36.30316803710216 40.0 36.0 41.0 29.0 41.0 17 36.26968389474916 40.0 36.0 41.0 29.0 41.0 18 36.23975141830906 40.0 36.0 41.0 29.0 41.0 19 36.31427877958194 40.0 37.0 41.0 29.0 41.0 20 36.28068462987765 40.0 37.0 41.0 29.0 41.0 21 36.2232066619055 40.0 37.0 41.0 29.0 41.0 22 36.1355552490095 40.0 37.0 41.0 29.0 41.0 23 36.073675240837524 40.0 37.0 41.0 29.0 41.0 24 36.0079938675267 40.0 36.0 41.0 29.0 41.0 25 35.885384563000166 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10434891216693742 1101 2 0.1776166201809204 1101 3 0.3998249431548473 1101 4 0.23248476751516733 1101 5 0.06692687448377654 1101 6 0.11857015903749613 1101 7 -0.06124021696439996 1101 8 -0.19638944798261804 1101 9 0.05811206710097849 1101 10 0.162774383717192 1101 11 0.05137688466428614 1101 12 -0.06326512425128072 1101 13 -0.11815612042565249 1101 14 -0.16118239134411283 1101 15 -0.22395552174958766 1101 16 -0.04016167155420902 1101 17 -0.0024138246465383872 1101 18 0.05823842793863321 1101 19 -0.10553175686499117 1101 20 0.07835771545425274 1101 21 0.03383806373748399 1101 22 -0.1304829584967777 1101 23 0.08363356746887973 1101 24 -0.048675245940323464 1101 25 0.043046626621809025 1102 1 -0.7288754681392398 1102 2 -0.576893894255825 1102 3 -0.4129524291799136 1102 4 -0.479717220126183 1102 5 -0.7155822198066204 1102 6 -0.7054007279622425 1102 7 -0.5441491500794555 1102 8 -0.6723300836108237 1102 9 -0.5221712859096499 1102 10 -0.5307614143966077 1102 11 -0.5999743676850144 1102 12 -0.5456358669821881 1102 13 -0.3607686825218295 1102 14 -0.47158580871216316 1102 15 -0.612789621041486 1102 16 -0.4223796921970475 1102 17 -0.5097667451042511 1102 18 -0.818933001196342 1102 19 -0.44924662303591845 1102 20 -0.44997899448367207 1102 21 -0.6588622712930743 1102 22 -0.4795571865035271 1102 23 -0.4654995515275502 1102 24 -0.461186904383851 1102 25 -0.5082350272851457 1103 1 -0.37080574896133456 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15 0.08281015953208026 2211 16 0.11256088409281517 2211 17 0.185234366159591 2211 18 0.21419293904207137 2211 19 0.06733176346035918 2211 20 0.02992383095283202 2211 21 0.32387652517606114 2211 22 0.3722451806811691 2211 23 0.12058485570632627 2211 24 0.24667146535996665 2211 25 0.12964069872986528 2212 1 -0.23833857385902846 2212 2 -0.12541321015482154 2212 3 -0.14493355955676535 2212 4 0.0015713467238853696 2212 5 0.08534659983877901 2212 6 -0.27591874233052494 2212 7 -0.10419704435194888 2212 8 -0.1151424702734758 2212 9 -0.12053717037179013 2212 10 -0.13824058604690492 2212 11 -0.2501918467795008 2212 12 -0.2679905572513164 2212 13 -0.21213181730742292 2212 14 -0.36705694482500917 2212 15 -0.258741114586563 2212 16 -0.08519835501571293 2212 17 0.04524607728824748 2212 18 -0.25259866213473003 2212 19 -0.21762559997104347 2212 20 -0.14290777297394897 2212 21 -0.36611045982468937 2212 22 -0.11514401995132317 2212 23 -0.051232099826940214 2212 24 0.06294542990890761 2212 25 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-0.0617698112986389 2214 12 0.23975920932197425 2214 13 0.29904154092475466 2214 14 0.286724567780027 2214 15 0.1178850914514129 2214 16 0.3634788936615365 2214 17 0.3315205870921716 2214 18 0.35110504156897093 2214 19 0.3407310760326894 2214 20 0.2237277701321787 2214 21 0.3340451579779895 2214 22 0.3129916787726117 2214 23 0.2728886595879345 2214 24 0.04897095122854722 2214 25 0.12851340946328804 2215 1 0.38288867110683356 2215 2 0.25463802000716385 2215 3 0.3148822124052053 2215 4 0.3818641425962994 2215 5 0.3460233484554749 2215 6 0.673243070232715 2215 7 0.7367820716087081 2215 8 0.47383803127163304 2215 9 0.5727564185654117 2215 10 0.5884916224106291 2215 11 0.2601225532715503 2215 12 0.20184107600635315 2215 13 0.34254179551287933 2215 14 0.3021393250357889 2215 15 0.46893000965113885 2215 16 0.4745800426287943 2215 17 0.24155467976224543 2215 18 0.35126775658524423 2215 19 0.0954298872168522 2215 20 0.4215538089624076 2215 21 0.37732624540133486 2215 22 0.09260249101940587 2215 23 0.22825714822002396 2215 24 0.20110506382291504 2215 25 0.16945339308109908 2216 1 0.0325696721097799 2216 2 0.08754572679318429 2216 3 -1.9895972492729435 2216 4 -1.0779251943541226 2216 5 -0.4551413071625774 2216 6 -0.09561078669491252 2216 7 0.0157747505375454 2216 8 -1.2842523271672235E-4 2216 9 0.043856523278378745 2216 10 0.03226804918003978 2216 11 0.10127695850631113 2216 12 0.2376953261598871 2216 13 0.09717655933432212 2216 14 0.03823392900427791 2216 15 -0.019371519511643953 2216 16 -0.04984304194128697 2216 17 -0.03202120179280854 2216 18 -0.13384157347267944 2216 19 -0.09576847979104741 2216 20 0.06209230754635087 2216 21 0.16909876642125 2216 22 0.0764183729279182 2216 23 -0.0954837556693775 2216 24 -0.049533375998059626 2216 25 -0.08073327876967085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13332.0 3 10.0 4 228.0 5 1395.0 6 1883.0 7 1460.0 8 1063.0 9 968.0 10 897.0 11 754.0 12 705.0 13 738.0 14 661.0 15 723.0 16 673.0 17 809.0 18 832.0 19 1096.0 20 1317.0 21 1697.0 22 2049.0 23 2590.0 24 3116.0 25 3670.0 26 4547.0 27 4890.0 28 5711.0 29 7026.0 30 8966.0 31 11677.0 32 15287.0 33 20379.0 34 27351.0 35 38346.0 36 56686.0 37 97588.0 38 231569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.656483438487186 19.515086037351907 10.44650614752307 28.38192437663784 2 13.971152518254746 20.802282345907564 40.52160414514294 24.704960990694754 3 18.404061537816695 24.64359376937465 29.246704890576346 27.705639802232305 4 11.878349330963228 17.445245150509265 38.16347092400937 32.51293459451814 5 12.69300215484876 38.5518857545987 35.644935406924155 13.110176683628389 6 31.493714714262182 38.36354779585701 17.680104012980596 12.462633476900217 7 28.145330448101635 31.729514767600875 21.631553117429977 18.493601666867516 8 24.781229098669126 38.56829267014884 19.55395359315161 17.096524638030424 9 25.87486837027115 15.927590115065776 19.957948340024693 38.23959317463838 10 14.375170255715354 30.176646852975896 34.167964684599106 21.280218206709648 11 34.254875904327676 23.089670539217654 24.03909454600699 18.61635901044768 12 22.874275127600242 25.470457684564167 31.69480012642159 19.960467061414 13 28.850015540802467 22.294416137289893 25.564794669330137 23.290773652577503 14 20.271421786382813 22.705207722180315 27.674929455480125 29.348441035956753 15 22.589680323675115 31.430851463225963 23.210802674475662 22.768665538623264 16 21.649772562578033 29.025904292935905 27.905742249229508 21.418580895256557 17 21.19094515687425 29.285230352303522 27.76014164780823 21.763682843014 18 21.047935350487528 28.431711507836727 29.64720755454977 20.873145587125975 19 22.87702902802224 27.647864107507054 27.487043556002572 21.988063308468135 20 22.959003712331018 27.951093618492507 28.266798909000745 20.823103760175734 21 22.33860855654944 27.178343526354574 27.54665446531171 22.936393451784273 22 22.379860622432073 28.62269050042868 27.390433551194455 21.607015325944797 23 21.88412908227677 28.259666241188498 27.714169470690024 22.142035205844707 24 22.389289823970778 27.562603786697494 28.54708227327572 21.50102411605601 25 22.204041861009298 27.924743782054527 27.998260738976388 21.87295361795979 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 160.0 2 178.0 3 196.0 4 196.0 5 196.0 6 351.5 7 507.0 8 507.0 9 507.0 10 790.0 11 1073.0 12 1073.0 13 1073.0 14 1837.5 15 2602.0 16 2602.0 17 2602.0 18 4619.5 19 6637.0 20 6637.0 21 6637.0 22 10533.0 23 14429.0 24 14429.0 25 14429.0 26 20581.0 27 26733.0 28 26733.0 29 26733.0 30 35026.0 31 43319.0 32 43319.0 33 43319.0 34 49696.5 35 56074.0 36 56074.0 37 56074.0 38 61148.0 39 66222.0 40 66222.0 41 66222.0 42 69821.0 43 73420.0 44 73420.0 45 73420.0 46 75218.0 47 77016.0 48 77016.0 49 77016.0 50 73933.5 51 70851.0 52 70851.0 53 70851.0 54 61854.0 55 52857.0 56 52857.0 57 52857.0 58 44834.5 59 36812.0 60 36812.0 61 36812.0 62 29942.0 63 23072.0 64 23072.0 65 23072.0 66 17516.5 67 11961.0 68 11961.0 69 11961.0 70 8718.0 71 5475.0 72 5475.0 73 5475.0 74 3831.0 75 2187.0 76 2187.0 77 2187.0 78 1479.0 79 771.0 80 771.0 81 771.0 82 494.5 83 218.0 84 218.0 85 218.0 86 144.0 87 70.0 88 70.0 89 70.0 90 48.0 91 26.0 92 26.0 93 26.0 94 13.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11699194501727814 2 0.11280118877785325 3 0.017112254644318294 4 0.0 5 0.004714600769353 6 0.34765815302895636 7 0.020604551510505702 8 0.010127660911943481 9 0.010476890598562221 10 0.0036669117094967774 11 0.004539985926043629 12 3.492296866187407E-4 13 5.23844529928111E-4 14 1.7461484330937035E-4 15 0.0027938374929499256 16 6.984593732374814E-4 17 1.7461484330937035E-4 18 1.7461484330937035E-4 19 1.7461484330937035E-4 20 5.23844529928111E-4 21 0.013096113248202776 22 0.0 23 0.0 24 0.0 25 0.005587674985899851 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 572689.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.77586497554958 #Duplication Level Percentage of deduplicated Percentage of total 1 92.82033698995161 79.617446926351 2 5.562192268575701 9.542037059947903 3 0.8431162176451023 2.169570685302671 4 0.2853905497945236 0.9791848505789167 5 0.1376101443981429 0.5901814582580491 6 0.08096076585826667 0.416668783234546 7 0.04666720431552414 0.2802043871308348 8 0.03688772286400647 0.2531261068510809 9 0.023807591663464547 0.18379050911265193 >10 0.1393633492013164 2.2751610203547075 >50 0.010609466376153587 0.6149737132724621 >100 0.01101745914443005 1.7154082455153152 >500 0.0018362431906164013 1.1539891828710847 >1k 2.0402702117960016E-4 0.2082570712187732 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1190 0.20779166353815073 No Hit GTCCTACAGTGGACATTTCTAAATT 957 0.16710640504706745 No Hit CTGTAGGACGTGGAATATGGCAAGA 922 0.16099488553123947 No Hit GTCCTACAGTGTGCATTTCTCATTT 834 0.14562877932001486 No Hit GGTATCAACGCAGAGTACTTTTTTT 802 0.140041104334115 No Hit GTCCTAAAGTGTGTATTTCTCATTT 680 0.11873809345037184 No Hit CTGTAGGACCTGGAATATGGCGAGA 632 0.11035658097152205 No Hit CTTTAGGACGTGAAATATGGCGAGG 631 0.1101819661282127 No Hit TATCAACGCAGAGTACTTTTTTTTT 631 0.1101819661282127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 1.7461484330937035E-4 0.0 0.0 0.0 12 0.0 1.7461484330937035E-4 0.0 0.0 0.0 13 0.0 1.7461484330937035E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 55 1.9783936E-4 13.800274 18 GTCTTAG 85 2.57618E-7 13.459018 1 CTAATAC 60 4.0038742E-4 12.696813 3 GTCCAGA 70 1.0534027E-4 12.257319 1 TGTAGGA 615 0.0 12.245172 2 ATAAGAC 70 1.0646778E-4 12.243356 3 GTAGGAC 585 0.0 12.208473 3 ACTGTTC 125 1.4279067E-9 12.145302 8 GTCTAGG 55 0.002984679 12.133508 1 GTGCTAC 55 0.002984679 12.133508 1 GTATTAT 95 9.928426E-7 12.042279 1 GACAGGT 95 1.0511994E-6 11.9854965 7 CTGTAGG 615 0.0 11.936214 1 GTCCTAC 475 0.0 11.841574 1 TCTTAGG 90 7.1587E-6 11.650998 2 AGGACTG 115 6.809387E-8 11.592742 5 TAGGACC 245 0.0 11.27166 4 AGGACGT 475 0.0 11.226655 5 TAGGACG 530 0.0 11.139657 4 TAACCTG 60 0.00576502 11.109711 5 >>END_MODULE