FastQCFastQC Report
Thu 26 May 2016
SRR1926890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569645
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9750.17115923074897524No Hit
CTGTAGGACGTGGAATATGGCAAGA7830.1374540283861001No Hit
GTCCTAAAGTGTGTATTTCTCATTT7630.13394306980663395No Hit
GTCCTACAGTGTGCATTTCTCATTT7430.1304321112271678No Hit
GTATCAACGCAGAGTACTTTTTTTT7160.1256923171448885No Hit
CTTTAGGACGTGAAATATGGCGAGG6010.10550430531295807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTA250.00597448819.0290645
ACTTAGT307.6763914E-419.0139928
CTGGACT755.671609E-815.2326424
GTACCAT551.9314987E-413.8393196
GAGTTTA500.001480353813.3203457
TAGGACC1800.013.2227794
CTAGGCT801.9455947E-613.0905534
GTCCTAG1403.6379788E-1212.9445031
ATAGGAC905.1573807E-712.7117933
GGACGTG3750.012.6860436
TGTAGGA4550.012.57210352
TAGGACG4300.012.39866354
CTGTAGG4600.012.2335911
GGCGAGG2250.012.22334219
AGGACGT4050.012.216195
TCCTAGA1251.3242243E-912.2033222
ATTATAG550.002983763612.1339843
TAGGACT1209.642463E-911.9005024
TCCAACG802.9098042E-511.85453518
GTGTAGG1302.4392648E-911.7391421
Produced by FastQC (version 0.11.5)