FastQCFastQC Report
Thu 26 May 2016
SRR1926890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569645
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12870.22593018458864733No Hit
CTGTAGGACGTGGAATATGGCAAGA10470.18379868163505342No Hit
GTCCTACAGTGGACATTTCTAAATT10090.17712786033406772No Hit
GGTATCAACGCAGAGTACTTTTTTT8150.14307156211324595No Hit
CTTTAGGACGTGAAATATGGCGAGG7580.13306533016176741No Hit
GTCCTACAGTGTGCATTTCTCATTT7520.1320120425879276No Hit
GTCCTAAAGTGTGTATTTCTCATTT7310.1283255360794881No Hit
TATCAACGCAGAGTACTTTTTTTTT6540.1148083455485434No Hit
CTGTAGGACCTGGAATATGGCGAGA6090.10690868874474453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG250.006062030318.98120918
TAGACAG852.6241287E-713.4386215
TTAGGGT655.3394484E-513.1801854
TAGGACC2750.012.8074124
TAAGAGT604.0165667E-412.6920314
GTCCTAA2350.012.5747271
GTTCTAT701.05443374E-412.2560131
AATGTCC1251.4297257E-912.1447758
GGACGTG4900.011.8491686
GACGTGG2250.011.8105317
AGGACGT5000.011.8035885
ATAGGAC657.870554E-411.7167523
TCTATAC657.870554E-411.7167523
AGGACCT4050.011.5168425
TGTAGGA5850.011.405352
TTAGGAC4100.011.3773953
ATAAGAC855.1970972E-511.1998373
TAGGACT855.201764E-511.1988514
GTAGGAC5700.011.1900123
GGTTAAT600.00572076611.1211981
Produced by FastQC (version 0.11.5)