##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926890_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 569645 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.94191470126131 31.0 30.0 33.0 26.0 34.0 2 30.26792476015764 31.0 30.0 34.0 26.0 34.0 3 30.32278875439967 31.0 30.0 34.0 26.0 34.0 4 33.92226913252991 35.0 35.0 37.0 30.0 37.0 5 33.88567792221471 37.0 35.0 37.0 30.0 37.0 6 33.81597837249515 37.0 35.0 37.0 30.0 37.0 7 33.809693756637905 37.0 35.0 37.0 29.0 37.0 8 33.84503331021952 37.0 35.0 37.0 30.0 37.0 9 35.40839645744279 39.0 35.0 39.0 30.0 39.0 10 35.3313502268957 39.0 35.0 39.0 30.0 39.0 11 35.34237639231451 39.0 35.0 39.0 30.0 39.0 12 35.25343152314161 39.0 35.0 39.0 30.0 39.0 13 35.25860140964987 39.0 35.0 39.0 30.0 39.0 14 36.36290321164936 40.0 36.0 41.0 30.0 41.0 15 36.342604604622174 40.0 36.0 41.0 30.0 41.0 16 36.324911128860954 40.0 36.0 41.0 29.0 41.0 17 36.305477973123615 40.0 36.0 41.0 29.0 41.0 18 36.283748650475296 40.0 36.0 41.0 29.0 41.0 19 36.35356581730727 40.0 37.0 41.0 29.0 41.0 20 36.31649887210455 40.0 37.0 41.0 29.0 41.0 21 36.26989616340001 40.0 37.0 41.0 29.0 41.0 22 36.17774930000263 40.0 37.0 41.0 29.0 41.0 23 36.111188547253114 40.0 37.0 41.0 29.0 41.0 24 36.044432936302435 40.0 37.0 41.0 29.0 41.0 25 35.927949863511486 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3015498791997935 1101 2 0.429638310077042 1101 3 0.587590311076692 1101 4 0.6238783247367294 1101 5 0.6649294224598705 1101 6 0.8843039955559533 1101 7 0.7672070334946 1101 8 0.7866117853896668 1101 9 0.8400336065696337 1101 10 0.8849669246983041 1101 11 0.7038302700247598 1101 12 0.6378183718427834 1101 13 0.858773180788674 1101 14 0.6661941291398179 1101 15 0.7831494781000004 1101 16 0.8600103204902609 1101 17 0.7249995255922954 1101 18 0.7378128270014912 1101 19 0.7907599610916733 1101 20 0.7272223879602606 1101 21 0.8671365023234188 1101 22 0.8167914397132421 1101 23 0.7915469134536863 1101 24 0.9648942604001576 1101 25 0.8805915923887753 1102 1 -0.3948008017178637 1102 2 -0.38490735635654616 1102 3 -0.18516798812751745 1102 4 -0.32213106493463073 1102 5 -0.4630631190777663 1102 6 -0.20374082181160702 1102 7 0.025065056336146085 1102 8 -0.392574443092343 1102 9 -0.1509596341988555 1102 10 -0.07645884029354022 1102 11 -0.1507546865230367 1102 12 0.03195981625393074 1102 13 -0.012751475422831504 1102 14 -0.023930067577126124 1102 15 0.08408511556545051 1102 16 -0.13765891002284292 1102 17 -0.24220321805636047 1102 18 0.03692546976112254 1102 19 -0.3779711014062457 1102 20 -0.2953777518859084 1102 21 -0.17483399679422007 1102 22 -0.13853270372896986 1102 23 -0.24516270596495815 1102 24 -0.25447276920061057 1102 25 -0.3386172778539631 1103 1 -0.5904309082481518 1103 2 -0.5190425632106752 1103 3 -0.4505903302491845 1103 4 -0.4002109819612727 1103 5 -0.6482801793713762 1103 6 -0.4075234470577982 1103 7 -0.5145252075345823 1103 8 -0.5695052011621087 1103 9 -0.5772463005345116 1103 10 -0.4851219430778926 1103 11 -0.5500929408827275 1103 12 -0.5129762710887746 1103 13 -0.5553856589020825 1103 14 -0.6064124274630345 1103 15 -0.636947306936662 1103 16 -0.49820960155944505 1103 17 -0.36436273213259085 1103 18 -0.5525867307431156 1103 19 -0.5307822101267234 1103 20 -0.41051294218949863 1103 21 -0.5907158921617253 1103 22 -0.49689458673874043 1103 23 -0.5461700819876398 1103 24 -0.6037511657845798 1103 25 -0.6493398191337576 1104 1 -0.5156562690083426 1104 2 -0.46191322083987174 1104 3 -0.19541458883904994 1104 4 -0.22906285173385754 1104 5 -0.41889267542391906 1104 6 -0.4433195862088368 1104 7 -0.32917978990392527 1104 8 -0.5407623014999956 1104 9 -0.586312234228096 1104 10 -0.5556001239818258 1104 11 -0.168571506705959 1104 12 -0.33238948601876217 1104 13 -0.5897695237357752 1104 14 -0.4848443421519093 1104 15 -0.27926105140174684 1104 16 -0.593910839129201 1104 17 -0.44939330204356054 1104 18 -0.47619651474104074 1104 19 -0.2815076610176632 1104 20 -0.6948002383618572 1104 21 -0.46464669072017273 1104 22 -0.3836429267742787 1104 23 -0.4835027810173429 1104 24 -0.6344742312601639 1104 25 -0.5365720189881955 1105 1 -0.04968164386416518 1105 2 -0.05476212510746237 1105 3 0.02257849074572249 1105 4 -0.0272174076738807 1105 5 -0.06479809641584922 1105 6 -0.06731035898853577 1105 7 -0.34186721781626517 1105 8 -0.17628709965455158 1105 9 -0.2933479897067599 1105 10 -0.08155168107525412 1105 11 -0.12474221028150367 1105 12 0.1762610330123806 1105 13 -0.03152269559275567 1105 14 0.1437600407777353 1105 15 -0.11086428092885114 1105 16 -0.0010619348616103252 1105 17 0.16306344536128847 1105 18 0.12933981743888978 1105 19 0.24559729861324797 1105 20 0.1298825759403357 1105 21 0.3092864119326535 1105 22 0.1469222404690882 1105 23 0.07128383951322093 1105 24 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2214 10 0.3433389667832145 2214 11 0.6711902954025959 2214 12 0.72890667311394 2214 13 0.8136670999270308 2214 14 0.5365028172719093 2214 15 0.6294285285460006 2214 16 0.5312351786840566 2214 17 0.6026572118285927 2214 18 0.5052053299660173 2214 19 0.6472430160996439 2214 20 0.6016021038191397 2214 21 0.6969954891737729 2214 22 0.9275362338582127 2214 23 0.8769705193721578 2214 24 0.6843181231181532 2214 25 0.6166167889536993 2215 1 0.5300447238415344 2215 2 0.22463839726599488 2215 3 0.4395085926073321 2215 4 0.38731351967877714 2215 5 0.5433780569563069 2215 6 0.34066841548784765 2215 7 0.09519942065341525 2215 8 0.4189147944050049 2215 9 0.23016306970988154 2215 10 0.17950864710362424 2215 11 0.020135600757370753 2215 12 -0.06936873814234445 2215 13 0.030266378804746807 2215 14 0.12798917531259946 2215 15 0.05253967855985309 2215 16 -0.009758030916096061 2215 17 0.15389942520044286 2215 18 0.08483233490704123 2215 19 -0.11443201652816981 2215 20 0.19362599309892659 2215 21 0.08312471437691471 2215 22 0.16989549101023727 2215 23 0.31042830409258215 2215 24 0.16268613787725883 2215 25 0.23378906042156444 2216 1 -0.16066391962644744 2216 2 -0.13086223017350562 2216 3 -1.7158449804455103 2216 4 -1.006556457871966 2216 5 -0.48491426839146357 2216 6 -0.01166205013595345 2216 7 -0.2629417046255256 2216 8 -0.0599182016493387 2216 9 -0.16043394204665162 2216 10 -0.36460375787253696 2216 11 -0.179223662272193 2216 12 -0.09381373633768675 2216 13 -0.18122040138582207 2216 14 -0.13844074422093655 2216 15 -0.07236045072732367 2216 16 -0.03542550250811871 2216 17 -0.1349339396073006 2216 18 -0.0580752521383161 2216 19 -0.18725522609072698 2216 20 -0.19868181095221615 2216 21 -0.05891686089291426 2216 22 -0.04001458903159971 2216 23 -0.15787510620592116 2216 24 -0.040826629371181866 2216 25 0.056572880416268845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12956.0 3 13.0 4 251.0 5 1433.0 6 1869.0 7 1417.0 8 1086.0 9 909.0 10 867.0 11 764.0 12 669.0 13 744.0 14 679.0 15 686.0 16 698.0 17 753.0 18 855.0 19 1020.0 20 1320.0 21 1700.0 22 2156.0 23 2672.0 24 3161.0 25 3627.0 26 4305.0 27 4830.0 28 5729.0 29 6817.0 30 8970.0 31 11545.0 32 15105.0 33 20010.0 34 27390.0 35 37841.0 36 56197.0 37 97891.0 38 230710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54785399089332 19.577950663669768 10.713965622380424 28.160229723056485 2 13.904993207775295 20.85643539126905 40.56460697358501 24.673964427370645 3 18.482804876121715 24.635721822979857 29.49885084917769 27.38262245172074 4 11.8480808222665 17.43401592219716 38.2933230345215 32.42458022101484 5 12.75986896389485 38.79574589107712 35.35762814818456 13.086756996843476 6 31.46748578201974 38.17833724045251 17.857501404625488 12.496675572902264 7 28.015907853844396 31.88400962196899 21.55812687654733 18.541955647639284 8 24.61160318477488 38.64392496291159 19.715582397710612 17.028889454602915 9 25.904474310243426 15.991467471931317 19.91309462152269 38.190963596302566 10 14.458184550456268 30.043783421286395 34.15914413418021 21.338887894077125 11 34.15580858885366 23.064242602989353 24.183756947589803 18.59619186056718 12 23.01435994663296 25.146408257847064 31.833614212485077 20.005617583034898 13 28.775116828582366 22.231838465832688 25.705272471288783 23.287772234296167 14 20.286038799739487 22.81885321157286 27.585522862564797 29.30958512612285 15 22.597826659410494 31.501325421765003 23.221915980548776 22.678931938275724 16 21.604911180589813 28.987481545329587 27.95454665147576 21.45306062260484 17 21.186214548033508 29.281445955719715 27.807718504890772 21.724620991356005 18 20.955136262455323 28.31738162616127 29.804120526090344 20.923361585293065 19 22.976265711677552 27.468225545958852 27.551611544133138 22.00389719823046 20 22.921304131059134 28.033516140131592 28.245405838114163 20.79977389069511 21 22.431888648166552 27.177122872562688 27.514036609302963 22.8769518699678 22 22.454160047046845 28.618701120873524 27.461489173081482 21.46564965899815 23 21.783292342748002 28.33335966561513 27.84410586981671 22.03924212182016 24 22.453106759473005 27.28734562753996 28.787227132687903 21.472320480299135 25 22.16401455035744 27.887424333927896 28.178326147104677 21.77023496860999 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 192.0 1 192.0 2 191.0 3 190.0 4 190.0 5 190.0 6 328.5 7 467.0 8 467.0 9 467.0 10 787.0 11 1107.0 12 1107.0 13 1107.0 14 1859.0 15 2611.0 16 2611.0 17 2611.0 18 4650.5 19 6690.0 20 6690.0 21 6690.0 22 10509.0 23 14328.0 24 14328.0 25 14328.0 26 20645.5 27 26963.0 28 26963.0 29 26963.0 30 35498.5 31 44034.0 32 44034.0 33 44034.0 34 50076.0 35 56118.0 36 56118.0 37 56118.0 38 60921.0 39 65724.0 40 65724.0 41 65724.0 42 69113.0 43 72502.0 44 72502.0 45 72502.0 46 74392.5 47 76283.0 48 76283.0 49 76283.0 50 73114.5 51 69946.0 52 69946.0 53 69946.0 54 61204.5 55 52463.0 56 52463.0 57 52463.0 58 44520.5 59 36578.0 60 36578.0 61 36578.0 62 29662.0 63 22746.0 64 22746.0 65 22746.0 66 17402.0 67 12058.0 68 12058.0 69 12058.0 70 8761.5 71 5465.0 72 5465.0 73 5465.0 74 3767.0 75 2069.0 76 2069.0 77 2069.0 78 1432.0 79 795.0 80 795.0 81 795.0 82 514.5 83 234.0 84 234.0 85 234.0 86 149.0 87 64.0 88 64.0 89 64.0 90 41.0 91 18.0 92 18.0 93 18.0 94 9.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10743533253166446 2 0.10743533253166446 3 0.01650150532349095 4 0.0 5 0.005441985798172546 6 0.3296790106118723 7 0.020188011831930412 8 0.010532875738398475 9 0.010532875738398475 10 0.0040376023663860824 11 0.0040376023663860824 12 8.777396448665397E-4 13 0.0012288355028131556 14 3.510958579466159E-4 15 0.0026332189345996187 16 0.0010532875738398476 17 1.7554792897330794E-4 18 0.0015799313607597714 19 8.777396448665397E-4 20 0.0015799313607597714 21 0.0110595195253184 22 0.0 23 3.510958579466159E-4 24 0.0 25 0.006495273372012393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 569645.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.01460601615655 #Duplication Level Percentage of deduplicated Percentage of total 1 92.55339995978308 78.68390833036717 2 5.760213062715545 9.79404488191761 3 0.892322751614541 2.275814015032888 4 0.28158786167411914 0.9575632447662892 5 0.13851168681451145 0.5887758241584479 6 0.07925843949177656 0.4042875004109261 7 0.059072176813842345 0.35153984868418814 8 0.0377157523832067 0.2565111863568988 9 0.026213640389696816 0.20056880789813553 >10 0.14565416070558088 2.3564502500762545 >50 0.01178478246434438 0.703247738462453 >100 0.01240497839109012 2.0805586162365537 >500 0.0012404978390816025 0.7591393649928395 >1k 6.202489195408013E-4 0.5875903906392828 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1287 0.22593018458864733 No Hit CTGTAGGACGTGGAATATGGCAAGA 1047 0.18379868163505342 No Hit GTCCTACAGTGGACATTTCTAAATT 1009 0.17712786033406772 No Hit GGTATCAACGCAGAGTACTTTTTTT 815 0.14307156211324595 No Hit CTTTAGGACGTGAAATATGGCGAGG 758 0.13306533016176741 No Hit GTCCTACAGTGTGCATTTCTCATTT 752 0.1320120425879276 No Hit GTCCTAAAGTGTGTATTTCTCATTT 731 0.1283255360794881 No Hit TATCAACGCAGAGTACTTTTTTTTT 654 0.1148083455485434 No Hit CTGTAGGACCTGGAATATGGCGAGA 609 0.10690868874474453 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 25 0.0060620303 18.981209 18 TAGACAG 85 2.6241287E-7 13.438621 5 TTAGGGT 65 5.3394484E-5 13.180185 4 TAGGACC 275 0.0 12.807412 4 TAAGAGT 60 4.0165667E-4 12.692031 4 GTCCTAA 235 0.0 12.574727 1 GTTCTAT 70 1.05443374E-4 12.256013 1 AATGTCC 125 1.4297257E-9 12.144775 8 GGACGTG 490 0.0 11.849168 6 GACGTGG 225 0.0 11.810531 7 AGGACGT 500 0.0 11.803588 5 ATAGGAC 65 7.870554E-4 11.716752 3 TCTATAC 65 7.870554E-4 11.716752 3 AGGACCT 405 0.0 11.516842 5 TGTAGGA 585 0.0 11.40535 2 TTAGGAC 410 0.0 11.377395 3 ATAAGAC 85 5.1970972E-5 11.199837 3 TAGGACT 85 5.201764E-5 11.198851 4 GTAGGAC 570 0.0 11.190012 3 GGTTAAT 60 0.005720766 11.121198 1 >>END_MODULE