##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926889_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 618222 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.66925796882026 31.0 28.0 31.0 16.0 34.0 2 28.050871693339932 31.0 28.0 31.0 16.0 34.0 3 27.636205117255614 31.0 27.0 31.0 15.0 34.0 4 31.458827088003986 35.0 32.0 37.0 17.0 37.0 5 31.642691460349194 35.0 32.0 37.0 17.0 37.0 6 30.614305217219705 35.0 30.0 37.0 11.0 37.0 7 30.423758779208764 35.0 30.0 37.0 11.0 37.0 8 30.547589700787096 35.0 31.0 37.0 10.0 37.0 9 32.77646702964307 37.0 32.0 39.0 17.0 39.0 10 32.93032761694019 37.0 33.0 39.0 17.0 39.0 11 33.097342055119356 37.0 33.0 39.0 17.0 39.0 12 32.966055882838205 37.0 33.0 39.0 16.0 39.0 13 33.083217679086154 37.0 33.0 39.0 17.0 39.0 14 34.08202069806639 39.0 34.0 40.0 17.0 41.0 15 33.65491845971189 39.0 33.0 40.0 10.0 41.0 16 33.735472047258106 39.0 33.0 40.0 11.0 41.0 17 33.9681603048743 39.0 33.0 40.0 13.0 41.0 18 34.01102678325909 39.0 34.0 41.0 11.0 41.0 19 33.37773162391503 39.0 33.0 40.0 10.0 41.0 20 33.663017492098305 39.0 33.0 40.0 10.0 41.0 21 33.66179301286593 39.0 33.0 40.0 2.0 41.0 22 33.68265930361585 39.0 34.0 40.0 2.0 41.0 23 33.666729750801494 39.0 34.0 40.0 2.0 41.0 24 32.350467954877054 38.0 32.0 40.0 2.0 41.0 25 32.92422139619748 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5343541867946549 1101 2 -0.5805479556012436 1101 3 -1.418543196549475 1101 4 -0.9447981847254141 1101 5 -0.5055637230240606 1101 6 -2.588588008672094 1101 7 -1.1306902319409566 1101 8 -0.29340394080857735 1101 9 -0.5775532589628583 1101 10 -9.735525832547136 1101 11 -4.556535627483175 1101 12 -2.0197685622748445 1101 13 -1.130205511800856 1101 14 -0.9789950474953812 1101 15 -2.280838400356256 1101 16 -0.8288055065023912 1101 17 -0.7328607892780212 1101 18 -0.5437259019520582 1101 19 -1.375643395675791 1101 20 -0.6054462638782852 1101 21 -0.6641412563137905 1101 22 -0.5107518108808264 1101 23 -0.5182960028438615 1101 24 -0.26348102162802434 1101 25 -0.08888286187081462 1102 1 -0.4482837557667452 1102 2 -0.48218108863378006 1102 3 -0.9314784233837763 1102 4 -0.6786707361232445 1102 5 -0.1802354907767878 1102 6 -1.217560236862525 1102 7 -0.6523813990685241 1102 8 0.05687525586277786 1102 9 -0.030110617302604226 1102 10 -0.0980598802173418 1102 11 -0.43117551344802507 1102 12 -0.5374883675649116 1102 13 -0.5560965269123628 1102 14 -0.9955308371125469 1102 15 -0.3169260429290688 1102 16 -0.1775991966807169 1102 17 -0.4600000456930715 1102 18 -0.7363704500508135 1102 19 -0.12908974763686842 1102 20 -0.43978456661203325 1102 21 -0.4871488178421757 1102 22 -0.43688887022484835 1102 23 -0.8544236131652454 1102 24 -4.178474471999863 1102 25 -2.6036073962927446 1103 1 -0.27773046085067676 1103 2 -0.5470145963259796 1103 3 -0.19986978498074137 1103 4 -0.11977650955935104 1103 5 -0.47835882955664744 1103 6 0.1355263815901253 1103 7 -0.25524904844275653 1103 8 0.19698047764842386 1103 9 0.23254497903387517 1103 10 0.2491650147299822 1103 11 -0.07958639857294258 1103 12 -0.35040188673330874 1103 13 -0.34920661091950933 1103 14 -0.20672082763069 1103 15 -0.05116697539329351 1103 16 0.30540985248615016 1103 17 -0.15935637573632988 1103 18 -0.23492134709510992 1103 19 -2.0443956570612265 1103 20 -0.7654636040111384 1103 21 -0.5740203532615169 1103 22 -0.4810244059614135 1103 23 -0.562851269397207 1103 24 1.1777366106928895 1103 25 0.4258528376397024 1104 1 -0.23186033716704557 1104 2 -0.13726281422693276 1104 3 -1.4411260159475816 1104 4 -0.41807692297230403 1104 5 -0.4527884727965805 1104 6 -1.9947956722045959 1104 7 -0.3228548260163784 1104 8 0.34415208655636675 1104 9 0.29623098934367675 1104 10 0.2441155923172147 1104 11 0.06110330353155291 1104 12 0.14794956531589776 1104 13 -0.19005453279637408 1104 14 -0.09629023166954909 1104 15 0.34375297901219426 1104 16 -0.23346967931942686 1104 17 -0.27504054865523386 1104 18 -0.17657057556105826 1104 19 -1.0694896532163156 1104 20 -0.44132062777812564 1104 21 -0.33577540183328125 1104 22 -0.1427573377908118 1104 23 -0.048606386020487946 1104 24 1.1757937399397278 1104 25 0.40520941056541204 1105 1 -0.4648474005206005 1105 2 -0.5980844436349493 1105 3 -0.037890469256538495 1105 4 -0.2970597202797087 1105 5 -0.16687473557394128 1105 6 -0.40787636696068574 1105 7 -0.3574762836744867 1105 8 0.19643054302330398 1105 9 -0.5662305746885679 1105 10 -0.3942012585650758 1105 11 -0.5793299494671444 1105 12 -0.5421360326231053 1105 13 -0.4161549787921075 1105 14 -0.5374492665458632 1105 15 -0.06020834955646848 1105 16 -0.7392066803443882 1105 17 -0.6632895306214905 1105 18 -0.8374420678461618 1105 19 -1.4024850270493872 1105 20 -0.7275113809845877 1105 21 -0.7192984911155378 1105 22 -0.7830687037566264 1105 23 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-0.22440836182246215 2214 24 -1.598890834744843 2214 25 -0.6952382898822904 2215 1 0.7929994906778575 2215 2 0.35476908974527177 2215 3 0.7504557577237421 2215 4 0.807225138150649 2215 5 0.7116749245026348 2215 6 1.5803760880417883 2215 7 1.1404477089279474 2215 8 -0.2612652710997949 2215 9 0.5759277436673003 2215 10 0.1878414076848216 2215 11 0.006692816285244874 2215 12 -0.11890662989851108 2215 13 0.3258105860538194 2215 14 0.4431711913739633 2215 15 0.4793764489640182 2215 16 0.5497412501601602 2215 17 0.3690722208306738 2215 18 -0.42313142212190513 2215 19 0.649850155675729 2215 20 -3.270049996806005 2215 21 -1.513474209382835 2215 22 -0.6422440313073565 2215 23 -0.44865376815742053 2215 24 -0.21531421236225157 2215 25 -0.1294190345107964 2216 1 -0.21979121291778014 2216 2 -0.8392801115359383 2216 3 0.060499194582437354 2216 4 -0.32818073931972336 2216 5 -0.31056495083917923 2216 6 -1.137656064399689 2216 7 -9.953564318957191 2216 8 -10.996520418087478 2216 9 -5.827497301017157 2216 10 -3.188736297443576 2216 11 -2.074990883944416 2216 12 -1.6566579958935108 2216 13 -1.5455949247748393 2216 14 -1.6096802117944335 2216 15 -2.747450521607888 2216 16 -1.5893880776124405 2216 17 -1.9715491350307595 2216 18 -1.5970652331079194 2216 19 -1.0074977007433006 2216 20 -1.9952601792611802 2216 21 -4.81402162583181 2216 22 -3.323301385274469 2216 23 -3.1648384537366923 2216 24 -3.315336109020212 2216 25 -2.5774185456551777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39616.0 3 5.0 4 304.0 5 1820.0 6 2212.0 7 1678.0 8 1303.0 9 1263.0 10 1225.0 11 1133.0 12 1145.0 13 1199.0 14 1174.0 15 1243.0 16 1273.0 17 1436.0 18 1683.0 19 2102.0 20 2645.0 21 3441.0 22 4226.0 23 5074.0 24 5927.0 25 6798.0 26 7685.0 27 8416.0 28 9508.0 29 11270.0 30 13214.0 31 16046.0 32 20775.0 33 27059.0 34 37018.0 35 51276.0 36 73255.0 37 114283.0 38 138492.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28160579564516 18.598413328687307 12.611200700145053 25.508780175522478 2 13.766354489377171 20.289040649985182 42.88175860053021 23.062846260107435 3 19.135356610740427 24.489862019822503 29.22588585865129 27.148895510785774 4 11.433187160772011 17.361836173419256 38.95911538891128 32.24586127689745 5 12.347890243132916 38.13855356382987 36.94092939647961 12.572626796557598 6 30.85784365277241 37.685839698546935 18.64100195284376 12.8153146958369 7 27.146543907372532 31.169149265763814 22.484675364279447 19.199631462584207 8 23.816088896530136 38.216197892421704 21.67882868343668 16.28888452761148 9 25.74988351324066 15.722800341694493 20.37968212057674 38.147634024488106 10 14.007870881299214 29.97601840854146 35.279154139170906 20.736956570988426 11 34.20145377007913 22.772064188495058 24.526586390602425 18.49989565082339 12 22.67947336575454 24.93520612702511 32.69024807721553 19.69507243000482 13 28.9908529976089 22.293512973083157 25.656743123570276 23.058890905737663 14 19.916066583725524 22.960998788239614 27.88229907670006 29.240635551334798 15 22.81659653567459 31.975202587162226 23.229940675813623 21.978260201349556 16 21.090098383571352 28.650797374496634 29.220616381730196 21.038487860201815 17 21.023458987218895 29.215499110176346 28.508979129590685 21.252062773014075 18 20.698625527398857 28.455271400098365 30.521671886728964 20.32443118577382 19 23.437636513663502 27.093371300985325 27.399809083113563 22.06918310223761 20 22.812745201135712 28.366404309877613 28.49680075714066 20.324049731846017 21 22.3267311515881 27.347851338474655 27.299803115530903 23.025614394406347 22 21.992635686505444 29.08353771783937 27.714539018566093 21.20928757708909 23 21.810666299960364 28.485799568041546 27.831875945414687 21.871658186583403 24 22.861732745106604 27.681276885569194 28.476580131331808 20.98041023799239 25 22.379309173327897 27.78885960697524 28.338906603637753 21.49292461605911 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 222.5 3 275.0 4 275.0 5 275.0 6 417.5 7 560.0 8 560.0 9 560.0 10 845.0 11 1130.0 12 1130.0 13 1130.0 14 2004.5 15 2879.0 16 2879.0 17 2879.0 18 5151.0 19 7423.0 20 7423.0 21 7423.0 22 11764.5 23 16106.0 24 16106.0 25 16106.0 26 23463.0 27 30820.0 28 30820.0 29 30820.0 30 41248.0 31 51676.0 32 51676.0 33 51676.0 34 57874.5 35 64073.0 36 64073.0 37 64073.0 38 68645.5 39 73218.0 40 73218.0 41 73218.0 42 76370.5 43 79523.0 44 79523.0 45 79523.0 46 81118.5 47 82714.0 48 82714.0 49 82714.0 50 78049.5 51 73385.0 52 73385.0 53 73385.0 54 63658.0 55 53931.0 56 53931.0 57 53931.0 58 45490.0 59 37049.0 60 37049.0 61 37049.0 62 29886.0 63 22723.0 64 22723.0 65 22723.0 66 17315.0 67 11907.0 68 11907.0 69 11907.0 70 8628.0 71 5349.0 72 5349.0 73 5349.0 74 3750.5 75 2152.0 76 2152.0 77 2152.0 78 1463.0 79 774.0 80 774.0 81 774.0 82 533.5 83 293.0 84 293.0 85 293.0 86 182.5 87 72.0 88 72.0 89 72.0 90 41.5 91 11.0 92 11.0 93 11.0 94 9.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19523730957487764 2 0.11824231424957378 3 0.12002161036003248 4 0.07424517406368586 5 0.026689441656880537 6 0.024101374587122425 7 0.27999650610945587 8 0.27255581328390127 9 0.020381028174345138 10 0.04011503958125075 11 0.016984190145287615 12 0.01746945272086726 13 0.015528402418548675 14 0.018278223680166673 15 0.01504313984296903 16 0.02118979913364455 17 0.01973401140690561 18 0.015204894034828912 19 0.024586637162702072 20 0.01730769852900738 21 0.01504313984296903 22 0.017145944337147497 23 0.01730769852900738 24 0.01504313984296903 25 0.01504313984296903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 618222.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.11272164356618 #Duplication Level Percentage of deduplicated Percentage of total 1 93.84181194718778 79.87132018788876 2 4.613262774568177 7.852947008008941 3 0.7661542998369852 1.9562843297413999 4 0.24642602850380757 0.8389595987909633 5 0.141654100083036 0.6028282995018653 6 0.08161634047542718 0.41679533210709346 7 0.05337655492399934 0.31801167030772143 8 0.03996927718375292 0.27215151705882423 9 0.023685493793748395 0.18143431562319493 >10 0.1583612440835921 2.528471497239394 >50 0.017507097158594064 1.0150313147832382 >100 0.01389133506698948 2.499573784022992 >500 0.0015223380893944955 0.9073162921953495 >1k 7.611690446972478E-4 0.7388748527303078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1345 0.21755938805154135 No Hit GTCCTACAGTGTGCATTTCTCATTT 1127 0.18229697422608707 No Hit CTGTAGGACGTGGAATATGGCAAGA 1085 0.17550329816797203 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1005 0.16256296281918145 No Hit GTACATGGGAAGCAGTGGTATCAAC 855 0.13829983404019916 No Hit CTTTAGGACGTGAAATATGGCGAGG 842 0.13619702954602067 No Hit GTATCAACGCAGAGTACTTTTTTTT 774 0.1251977444995487 No Hit CCCATGTACTCTGCGTTGATACCAC 713 0.1153307387960959 No Hit CTGTAGGACCTGGAATATGGCGAGA 703 0.11371319687749708 No Hit GATATACACTGTTCTACAAATCCCG 626 0.10125812410428617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.235083837197641E-4 0.0 5 0.0 0.0 0.0 3.235083837197641E-4 0.0 6 0.0 0.0 0.0 3.235083837197641E-4 0.0 7 0.0 0.0 0.0 3.235083837197641E-4 0.0 8 0.0 0.0 0.0 3.235083837197641E-4 0.0 9 0.0 0.0 0.0 3.235083837197641E-4 0.0 10 0.0 0.0 0.0 3.235083837197641E-4 0.0 11 0.0 0.0 0.0 3.235083837197641E-4 0.0 12 0.0 0.0 0.0 3.235083837197641E-4 0.0 13 0.0 0.0 0.0 3.235083837197641E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCGTC 35 0.0021497982 16.306509 8 CCAACGA 80 7.878043E-9 15.408451 19 AGGACCG 45 6.652148E-4 14.807462 5 TCCAACG 125 7.2759576E-12 13.654258 18 GTACATA 85 2.5635018E-7 13.464946 1 TAGATAG 60 4.0172125E-4 12.69211 5 GTATTAA 100 1.364715E-7 12.398972 1 TAGGACC 300 0.0 12.379833 4 ATAGGAC 70 1.0549337E-4 12.255739 3 CCCCTAT 55 0.0029753046 12.138853 1 ACTGTTC 390 0.0 11.951139 8 TAGGACT 105 2.625875E-7 11.790318 4 TGAACTG 105 2.6397538E-7 11.785531 5 GTAGGAC 740 0.0 11.72208 3 ACTGGCC 65 7.926065E-4 11.707237 8 TGTAGGA 800 0.0 11.676996 2 GTTCTAT 90 7.1192735E-6 11.657152 1 GTATAGA 100 1.8337323E-6 11.445205 1 CTGTAGG 770 0.0 11.395658 1 GTCCTAT 85 5.1006664E-5 11.220788 1 >>END_MODULE