FastQCFastQC Report
Thu 26 May 2016
SRR1926889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618222
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA14380.23260252789451039No Hit
GTCCTACAGTGGACATTTCTAAATT13320.21545658355736289No Hit
GTATCAACGCAGAGTACTTTTTTTT12970.20979518684226703No Hit
GTCCTACAGTGTGCATTTCTCATTT10820.17501803559239237No Hit
GTACATGGGAAGCAGTGGTATCAAC10460.16919488468543664No Hit
CTTTAGGACGTGAAATATGGCGAGG10350.1674155885749779No Hit
GTCCTAAAGTGTGTATTTCTCATTT10340.16725383438311806No Hit
CTGTAGGACCTGGAATATGGCGAGA8710.14088790110995728No Hit
CCCATGTACTCTGCGTTGATACCAC8200.13263843732510328No Hit
GGTATCAACGCAGAGTACTTTTTTT7560.12228616904607084No Hit
GATATACACTGTTCTACAAATCCCG7520.1216391522786313No Hit
TATCAACGCAGAGTACTTTTTTTTT7130.1153307387960959No Hit
GTGTATATCAATGAGTTACAATGAA6580.10643425824380238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT653.4029235E-614.59926919
TGTAGGA8450.013.1916272
TCCAACG1700.012.83876918
TAGGACC3750.012.69484
GGCGAGG2750.012.42265119
GTAGGAC8150.012.3842763
GCCGGTT701.10095425E-412.19983111
CCGGTTT701.10095425E-412.19983112
TGCCGGT701.10095425E-412.19983110
TAGGACT951.0089789E-612.0266534
CTGTAGG8600.011.9654691
CTACACT1209.638825E-911.9013754
GTGTAGG1052.6076668E-711.7966451
CCAACGA1052.7485112E-711.74893619
CTAATGT657.863634E-411.7182774
GGACCTT907.258517E-611.6369016
ACTGTTC2700.011.5983098
AGGACCT5200.011.5351795
GCAGGAC752.0261976E-411.4262483
GTCCTAC6800.011.34961