FastQCFastQC Report
Thu 26 May 2016
SRR1926888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685852
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16600.24203472469279086No Hit
CTGTAGGACGTGGAATATGGCAAGA12860.18750400961140304No Hit
GTCCTACAGTGTGCATTTCTCATTT12510.18240086782571166No Hit
GTCCTAAAGTGTGTATTTCTCATTT12150.17715192198900054No Hit
CTTTAGGACGTGAAATATGGCGAGG9990.14565824696873378No Hit
GTATCAACGCAGAGTACTTTTTTTT9020.13151525401981767No Hit
CTGTAGGACCTGGAATATGGCGAGA8100.11810128132600035No Hit
GTACATGGGAAGCAGTGGTATCAAC7020.10235444381586699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA551.98928E-413.79208519
CGAAATC655.5412267E-513.12900313
CTCTAGG801.8969477E-613.1210861
TAGGACA2650.012.93316654
TAGGACC3050.012.797714
CTAGGAC603.952465E-412.7169443
ACGAAAT604.1550872E-412.64182312
TCCAACG854.018264E-612.27089818
AATACCA701.0706643E-412.2367335
GTAGGAC7900.012.1937793
TTAGACA1103.6885467E-812.1166784
GACTGTT550.003023552212.113137
TGTAGGA8350.011.8793012
GTCCTAA3500.011.7237771
CTGTAGG8250.011.4511311
AGGACAT2850.011.3541625
GGCGAGG2600.010.94083619
GTTATAG700.001434949910.9058381
AGGACCT5050.010.742485
GTCCTAG1252.097895E-710.6877211
Produced by FastQC (version 0.11.5)