##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926888_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 685852 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.840663875005102 31.0 28.0 31.0 16.0 34.0 2 28.2517161136805 31.0 28.0 31.0 16.0 34.0 3 27.834820048640232 31.0 28.0 31.0 16.0 34.0 4 31.678284819465425 35.0 32.0 37.0 19.0 37.0 5 31.834207671625947 35.0 32.0 37.0 19.0 37.0 6 30.87327149297516 35.0 31.0 37.0 11.0 37.0 7 30.69257070038434 35.0 31.0 37.0 11.0 37.0 8 30.780903168613637 35.0 32.0 37.0 11.0 37.0 9 32.999438654403576 37.0 33.0 39.0 17.0 39.0 10 33.15789558097082 37.0 33.0 39.0 17.0 39.0 11 33.31008439138473 38.0 33.0 39.0 17.0 39.0 12 33.19030198935047 38.0 33.0 39.0 17.0 39.0 13 33.29587287053184 38.0 34.0 39.0 17.0 39.0 14 34.31172760303972 39.0 34.0 41.0 17.0 41.0 15 33.893794871196704 39.0 33.0 41.0 11.0 41.0 16 33.98681931378781 39.0 33.0 41.0 16.0 41.0 17 34.21800767512524 39.0 34.0 41.0 17.0 41.0 18 34.25505356840834 39.0 34.0 41.0 17.0 41.0 19 33.6330432804745 39.0 33.0 41.0 10.0 41.0 20 33.91297976822988 39.0 34.0 41.0 10.0 41.0 21 33.90690702950491 39.0 34.0 41.0 10.0 41.0 22 33.92253576573371 39.0 34.0 41.0 7.0 41.0 23 33.925276881892884 39.0 34.0 41.0 2.0 41.0 24 32.63170334124563 39.0 32.0 40.0 2.0 41.0 25 33.20215731673889 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4782758062170558 1101 2 -0.4606966685071292 1101 3 -1.5257917564521541 1101 4 -0.954663924841352 1101 5 -0.3787219023984001 1101 6 -2.36248937303969 1101 7 -0.9607605121657521 1101 8 -0.4531752955821098 1101 9 -0.6038500364061932 1101 10 -9.177290314927166 1101 11 -4.157973214067859 1101 12 -1.6132655632975066 1101 13 -0.9398805546600215 1101 14 -0.8224753680457582 1101 15 -1.9398872340706568 1101 16 -0.48391514115791523 1101 17 -0.42999160335207876 1101 18 -0.3160492912804358 1101 19 -1.08333137185614 1101 20 -0.20513777610239714 1101 21 -0.2840549624979971 1101 22 -0.09974408372707444 1101 23 -0.22548330725346943 1101 24 -0.20918329643831868 1101 25 -0.14344997794552938 1102 1 -1.2144319812842674 1102 2 -1.2902749861373763 1102 3 -1.605030257675299 1102 4 -1.4901293255728163 1102 5 -1.2022489205881435 1102 6 -2.2212788225032014 1102 7 -1.2757216379438425 1102 8 -0.8345050119577238 1102 9 -0.9234741311575689 1102 10 -0.9812276317581308 1102 11 -1.3351185624826165 1102 12 -1.0868296391567398 1102 13 -1.3685452675236327 1102 14 -1.5083377460339875 1102 15 -0.9299218696702383 1102 16 -1.0649348325862036 1102 17 -1.19929138294372 1102 18 -1.52505551600008 1102 19 -0.7664074006303281 1102 20 -1.0974147640162712 1102 21 -1.4089262294827662 1102 22 -1.409461912583616 1102 23 -1.8210877347972456 1102 24 -4.794260439364663 1102 25 -3.116796551927653 1103 1 -0.7799227318439463 1103 2 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0.9194169906157299 2215 5 0.8284650943240237 2215 6 1.7854602987740193 2215 7 1.433832260703955 2215 8 -0.31171045447847234 2215 9 0.4005723745924641 2215 10 -0.08169437424938764 2215 11 0.11698978243502722 2215 12 -0.21108171380183904 2215 13 0.26345945045100905 2215 14 0.4697742216141876 2215 15 0.30000862445335486 2215 16 0.7742251994286207 2215 17 0.16076000928004675 2215 18 -0.5496867136073931 2215 19 0.6747240707880096 2215 20 -3.2669383328270882 2215 21 -1.6987522541678857 2215 22 -0.4894762350066344 2215 23 -0.290954199265002 2215 24 -0.24858623236352884 2215 25 -0.03126978163404459 2216 1 -0.7217220712983909 2216 2 -1.4376325340430185 2216 3 -0.3792883262715421 2216 4 -0.6012501806015926 2216 5 -0.950568586671583 2216 6 -1.7429214221616967 2216 7 -9.89359797919645 2216 8 -11.18943976752827 2216 9 -6.099490321051533 2216 10 -3.639086265314212 2216 11 -2.6086645287115076 2216 12 -2.2422138329295294 2216 13 -2.184632288203691 2216 14 -2.284942506031456 2216 15 -3.452603320858131 2216 16 -2.4446266343443277 2216 17 -2.65566341321545 2216 18 -2.4972029958154174 2216 19 -1.920503148675106 2216 20 -2.652731499897545 2216 21 -5.052083258805869 2216 22 -3.8037254621335457 2216 23 -3.8513442855909332 2216 24 -3.8281985045880127 2216 25 -3.3370558431981827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41488.0 3 6.0 4 339.0 5 1950.0 6 2419.0 7 1722.0 8 1431.0 9 1353.0 10 1319.0 11 1297.0 12 1302.0 13 1220.0 14 1261.0 15 1307.0 16 1454.0 17 1555.0 18 1802.0 19 2174.0 20 2800.0 21 3652.0 22 4588.0 23 5331.0 24 6386.0 25 7313.0 26 8262.0 27 9092.0 28 10142.0 29 11915.0 30 14306.0 31 17549.0 32 22499.0 33 29725.0 34 39739.0 35 55401.0 36 80630.0 37 127432.0 38 163691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99300740177847 18.595342651926437 12.755463652372148 25.656186293922943 2 13.810951171900665 20.092478898779845 42.840477549703394 23.256092379616096 3 19.039067188204942 24.401771361241178 29.141024124795408 27.418137325758472 4 11.537591752589353 17.17777716175739 38.86487219927546 32.4197588863778 5 12.526814841604676 37.983086415612185 36.72864036539642 12.761458377386722 6 31.038143626298627 37.43882378332974 18.581090934545095 12.941941655826541 7 27.399153304525214 31.13792069724422 22.42207550066902 19.040850497561546 8 24.008130319071157 37.964203199485276 21.532916093937356 16.494750387506215 9 25.90613657342933 15.682451537825507 20.445620139382072 37.9657917493631 10 14.320573499704645 29.5937224243927 34.99150397818017 21.09420009772248 11 34.037514527656135 22.700766010315547 24.77102080754082 18.4906986544875 12 23.131495634700162 24.858949848997227 32.277990926709336 19.731563589593275 13 28.831972561949243 22.440259973197737 25.628970941856004 23.098796522997013 14 20.056375654933163 22.859117734124478 27.908428204159787 29.17607840678258 15 22.650908109921037 32.04392175162045 23.09493777022741 22.2102323682311 16 21.190883465088234 28.425226360312827 29.041638048073402 21.342252126525533 17 21.1357915950897 29.213902604769725 28.30160614341837 21.348699656722207 18 20.73727880542168 28.236640831771815 30.40677199915423 20.619308363652273 19 23.63354508280396 27.05434903079409 27.28957760938931 22.02252827701264 20 23.05930080290715 28.063835930459437 28.36554586311789 20.511317403515523 21 22.31904853564783 27.347600632287495 27.21198546438717 23.12136536767751 22 22.399675684031084 29.01344657171481 27.47148746410869 21.11539028014542 23 21.76492890995261 28.485162231133792 27.922712358731317 21.827196500182282 24 23.152605200338893 27.351286085717664 28.428917862293535 21.067190851649915 25 22.232964545956975 27.847165411856317 28.49724612510773 21.42262391707898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 178.0 2 251.0 3 324.0 4 324.0 5 324.0 6 465.5 7 607.0 8 607.0 9 607.0 10 953.5 11 1300.0 12 1300.0 13 1300.0 14 2249.0 15 3198.0 16 3198.0 17 3198.0 18 5601.5 19 8005.0 20 8005.0 21 8005.0 22 12763.0 23 17521.0 24 17521.0 25 17521.0 26 25857.5 27 34194.0 28 34194.0 29 34194.0 30 44988.0 31 55782.0 32 55782.0 33 55782.0 34 63176.0 35 70570.0 36 70570.0 37 70570.0 38 75497.0 39 80424.0 40 80424.0 41 80424.0 42 83937.0 43 87450.0 44 87450.0 45 87450.0 46 89234.0 47 91018.0 48 91018.0 49 91018.0 50 86129.0 51 81240.0 52 81240.0 53 81240.0 54 71133.0 55 61026.0 56 61026.0 57 61026.0 58 51788.0 59 42550.0 60 42550.0 61 42550.0 62 34356.0 63 26162.0 64 26162.0 65 26162.0 66 20101.5 67 14041.0 68 14041.0 69 14041.0 70 10220.5 71 6400.0 72 6400.0 73 6400.0 74 4480.5 75 2561.0 76 2561.0 77 2561.0 78 1717.0 79 873.0 80 873.0 81 873.0 82 588.0 83 303.0 84 303.0 85 303.0 86 198.5 87 94.0 88 94.0 89 94.0 90 58.0 91 22.0 92 22.0 93 22.0 94 14.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22701690743775627 2 0.13691000390754857 3 0.13924286872386463 4 0.06546601890786934 5 0.019100330683587713 6 0.01866291853052845 7 0.2940867709068429 8 0.29044166963134904 9 0.016184249663192644 10 0.034555560091681585 11 0.01326816864279757 12 0.015017817255034614 13 0.012976560540758065 14 0.013851384846876586 15 0.012684952438718556 16 0.015892641561153133 17 0.016038445612172887 18 0.012684952438718556 19 0.01982935093868648 20 0.013705580795856832 21 0.012830756489738311 22 0.014726209152995107 23 0.014872013204014862 24 0.012976560540758065 25 0.01326816864279757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 685852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.20599932553642 #Duplication Level Percentage of deduplicated Percentage of total 1 93.84606203144 79.96247498155125 2 4.539843077259601 7.736437323580454 3 0.7907587128807675 2.0213215906914215 4 0.26969406433002757 0.9191820905362201 5 0.14309853519994825 0.609642684686602 6 0.08614838004361329 0.4404215287135302 7 0.052429933421230965 0.3127141410209128 8 0.04798218272816883 0.32706958633392946 9 0.029075968220942 0.2229702235760612 >10 0.16353877787612683 2.5772354467217795 >50 0.01628431547254602 0.9740348848640211 >100 0.013198518488101506 2.3771266187216855 >500 0.001199865315719066 0.7289407069998991 >1k 6.856373232680377E-4 0.790428192002295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1660 0.24203472469279086 No Hit CTGTAGGACGTGGAATATGGCAAGA 1286 0.18750400961140304 No Hit GTCCTACAGTGTGCATTTCTCATTT 1251 0.18240086782571166 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1215 0.17715192198900054 No Hit CTTTAGGACGTGAAATATGGCGAGG 999 0.14565824696873378 No Hit GTATCAACGCAGAGTACTTTTTTTT 902 0.13151525401981767 No Hit CTGTAGGACCTGGAATATGGCGAGA 810 0.11810128132600035 No Hit GTACATGGGAAGCAGTGGTATCAAC 702 0.10235444381586699 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4580405101975353E-4 2 0.0 0.0 0.0 0.0 1.4580405101975353E-4 3 0.0 0.0 0.0 0.0 1.4580405101975353E-4 4 0.0 0.0 0.0 0.0 1.4580405101975353E-4 5 0.0 0.0 0.0 0.0 1.4580405101975353E-4 6 0.0 0.0 0.0 0.0 1.4580405101975353E-4 7 0.0 0.0 0.0 0.0 1.4580405101975353E-4 8 0.0 0.0 0.0 0.0 1.4580405101975353E-4 9 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 10 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 11 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 12 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 13 0.0 0.0 0.0 1.4580405101975353E-4 2.9160810203950706E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 55 1.98928E-4 13.792085 19 CGAAATC 65 5.5412267E-5 13.129003 13 CTCTAGG 80 1.8969477E-6 13.121086 1 TAGGACA 265 0.0 12.9331665 4 TAGGACC 305 0.0 12.79771 4 CTAGGAC 60 3.952465E-4 12.716944 3 ACGAAAT 60 4.1550872E-4 12.641823 12 TCCAACG 85 4.018264E-6 12.270898 18 AATACCA 70 1.0706643E-4 12.236733 5 GTAGGAC 790 0.0 12.193779 3 TTAGACA 110 3.6885467E-8 12.116678 4 GACTGTT 55 0.0030235522 12.11313 7 TGTAGGA 835 0.0 11.879301 2 GTCCTAA 350 0.0 11.723777 1 CTGTAGG 825 0.0 11.451131 1 AGGACAT 285 0.0 11.354162 5 GGCGAGG 260 0.0 10.940836 19 GTTATAG 70 0.0014349499 10.905838 1 AGGACCT 505 0.0 10.74248 5 GTCCTAG 125 2.097895E-7 10.687721 1 >>END_MODULE