FastQCFastQC Report
Thu 26 May 2016
SRR1926888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685852
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16200.2362025626520007No Hit
CTGTAGGACGTGGAATATGGCAAGA16020.23357808973364516No Hit
GTATCAACGCAGAGTACTTTTTTTT14700.21433195499903768No Hit
CTTTAGGACGTGAAATATGGCGAGG12700.18517114479508698No Hit
GTCCTAAAGTGTGTATTTCTCATTT12120.17671450983594128No Hit
GTCCTACAGTGTGCATTTCTCATTT11870.17306940856044745No Hit
CTGTAGGACCTGGAATATGGCGAGA9910.14449181456057575No Hit
GGTATCAACGCAGAGTACTTTTTTT8980.13093203781573867No Hit
GTACATGGGAAGCAGTGGTATCAAC8100.11810128132600035No Hit
TATCAACGCAGAGTACTTTTTTTTT7820.11401876789744725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA905.129059E-712.7186181
TAGGACC4000.012.379784
ACTATAC1353.5470293E-1011.9926863
TGTAGGA9350.011.9364572
GACAGGT802.899712E-511.8593697
GTAAGAC657.848227E-411.7213593
TAGACTG657.8530493E-411.7205015
CTGTAGG9150.011.6761091
AATCCCG1406.91216E-1011.52053119
TATTAGG751.9933209E-411.4467562
GTAGGAC9150.011.2409753
CAGGACT855.1837706E-511.203424
TGGCGAG7200.011.06874518
AATGTCC1553.1832315E-1011.0169338
GGCGAGA4750.010.78578519
GGCGAGG2650.010.740561519
GTTCTAA803.6250817E-410.7313341
GTCCTAA4000.010.7313341
GTCCAAG803.6250817E-410.7313341
TTAGACT803.6819858E-410.713274