##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926888_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 685852 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.085428051532983 31.0 30.0 33.0 26.0 34.0 2 30.362515528131432 31.0 30.0 34.0 26.0 34.0 3 30.433488566046318 31.0 30.0 34.0 26.0 34.0 4 34.01382368207718 37.0 35.0 37.0 30.0 37.0 5 34.01806366388084 37.0 35.0 37.0 30.0 37.0 6 33.98845523524026 37.0 35.0 37.0 30.0 37.0 7 33.99776773997889 37.0 35.0 37.0 30.0 37.0 8 34.02634241789774 37.0 35.0 37.0 30.0 37.0 9 35.595017583968556 39.0 35.0 39.0 30.0 39.0 10 35.48848877017199 39.0 35.0 39.0 30.0 39.0 11 35.50418603430478 39.0 35.0 39.0 30.0 39.0 12 35.40814053177653 39.0 35.0 39.0 30.0 39.0 13 35.418747776488225 39.0 35.0 39.0 30.0 39.0 14 36.553132454232106 40.0 37.0 41.0 30.0 41.0 15 36.555020616692815 40.0 37.0 41.0 30.0 41.0 16 36.5525871470813 40.0 37.0 41.0 30.0 41.0 17 36.5258787610155 40.0 37.0 41.0 30.0 41.0 18 36.505934224876505 40.0 37.0 41.0 30.0 41.0 19 36.57063914663805 40.0 37.0 41.0 30.0 41.0 20 36.52688480896753 40.0 37.0 41.0 30.0 41.0 21 36.47952911123683 40.0 37.0 41.0 30.0 41.0 22 36.396880084916276 40.0 37.0 41.0 30.0 41.0 23 36.32932906807883 40.0 37.0 41.0 30.0 41.0 24 36.26377994086188 40.0 37.0 41.0 30.0 41.0 25 36.1496139108729 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2495543913984264 1101 2 -0.13533005071685622 1101 3 0.11885267835488733 1101 4 0.16434528834479778 1101 5 -0.18961283760175007 1101 6 -0.22886353991805208 1101 7 -0.20829985552277464 1101 8 -0.39320674124819277 1101 9 -0.23358975582785746 1101 10 -0.18291734726852127 1101 11 -0.24031118465608614 1101 12 -0.19530098661387285 1101 13 -0.329116876266788 1101 14 -0.20264534138522095 1101 15 -0.2021704572073304 1101 16 -0.2627684574191349 1101 17 -0.36135353916178303 1101 18 -0.27525908152060197 1101 19 -0.30489663100483 1101 20 -0.1871533188374883 1101 21 -0.2170784644920971 1101 22 -0.5006026153194085 1101 23 -0.36664762182725497 1101 24 -0.3123616918627192 1101 25 -0.24321990043368658 1102 1 -0.8162935586907949 1102 2 -0.8455778284677216 1102 3 -0.5698503032131583 1102 4 -0.6946824927969644 1102 5 -0.7415085917132131 1102 6 -0.682151984222557 1102 7 -0.6062575661823004 1102 8 -0.6781128860395285 1102 9 -0.8825678845366554 1102 10 -0.6954660914252742 1102 11 -0.8781032257343924 1102 12 -0.740339376421808 1102 13 -0.6798151590192276 1102 14 -0.8740101777743945 1102 15 -0.9041802908111265 1102 16 -0.7353869571890428 1102 17 -0.8912520544329325 1102 18 -0.952336127564152 1102 19 -0.8939605009881149 1102 20 -0.9386053497430993 1102 21 -0.9532501408066807 1102 22 -0.9642071397459802 1102 23 -0.9357584799666228 1102 24 -0.7935804774368691 1102 25 -0.7341414980309935 1103 1 -0.7540854757403999 1103 2 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0.33470203549870803 2214 11 0.3447215649142237 2214 12 0.43519288198992 2214 13 0.4579438662320996 2214 14 0.37189772914227603 2214 15 0.5225245506415206 2214 16 0.31177899549431487 2214 17 0.371195580811289 2214 18 0.3875583894745773 2214 19 0.40601840241962606 2214 20 0.53246863901974 2214 21 0.47662693925273913 2214 22 0.6853087149889134 2214 23 0.5518250991348381 2214 24 0.3215793615401594 2214 25 0.3416322948349162 2215 1 0.5889096933417264 2215 2 0.2337641825689687 2215 3 0.38463640642077834 2215 4 0.32463003089821996 2215 5 0.6063636859732924 2215 6 0.4634834679868831 2215 7 0.27990276070594433 2215 8 0.4604034794350085 2215 9 0.19211596903762995 2215 10 0.12970346078837736 2215 11 0.2081025959093168 2215 12 0.19438828691700394 2215 13 0.17235468806784127 2215 14 -0.057207448089066304 2215 15 0.14667947581762775 2215 16 0.0985571853611944 2215 17 0.29893034583665923 2215 18 0.1171512139413835 2215 19 0.044683081302629546 2215 20 0.08371656777659808 2215 21 0.22041092326509926 2215 22 0.10314953071191724 2215 23 0.10187410775822059 2215 24 0.12744652454299654 2215 25 0.262086649742713 2216 1 -0.28951151092217486 2216 2 -0.1304609365900724 2216 3 -2.0926177632127363 2216 4 -1.4650372791451716 2216 5 -0.6022687578282699 2216 6 -0.4548838180231769 2216 7 -0.5072529916940312 2216 8 -0.35250376235970293 2216 9 -0.22727012118737377 2216 10 -0.4894749874347184 2216 11 -0.5268052937403809 2216 12 -0.3228171890996734 2216 13 -0.5488355439508226 2216 14 -0.8830613403689966 2216 15 -0.4378379841728517 2216 16 -0.44970278660489527 2216 17 -0.741834511165024 2216 18 -0.5262240209806848 2216 19 -0.45521690421766436 2216 20 -0.36605227935500295 2216 21 -0.21200765076725503 2216 22 -0.47809208187975827 2216 23 -0.543387967497452 2216 24 -0.6258826887908739 2216 25 -0.7315131699326685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14547.0 3 8.0 4 232.0 5 1635.0 6 2060.0 7 1563.0 8 1242.0 9 1027.0 10 958.0 11 863.0 12 793.0 13 715.0 14 778.0 15 738.0 16 810.0 17 919.0 18 963.0 19 1091.0 20 1499.0 21 1916.0 22 2446.0 23 2964.0 24 3619.0 25 4238.0 26 5151.0 27 5606.0 28 6620.0 29 8039.0 30 10267.0 31 13241.0 32 17650.0 33 23574.0 34 31837.0 35 44783.0 36 66599.0 37 116318.0 38 288543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.379320412301304 19.62576985342312 10.921225692776844 28.07368404149873 2 13.850207275062768 20.69714485899457 40.86836573830793 24.58428212763473 3 18.498642314860454 24.874950052936594 29.21589672843089 27.41051090377206 4 11.871365834028333 17.655558342032975 38.10457649755341 32.36849932638528 5 12.680790132529612 38.7752275050342 35.473579378515005 13.070402983921179 6 31.487089902501257 38.392909395226894 17.69944520523895 12.420555497032902 7 27.953653877003397 31.771740240043165 21.714281547593007 18.560324335360427 8 24.773685714785675 38.90841270859703 19.66294920177552 16.654952374841784 9 25.887321150469834 15.866643724153217 20.108286719201136 38.13774840617582 10 14.62303629804818 30.05386237245233 34.06918996474304 21.253911364756455 11 33.93840595031007 22.885101332287373 24.648264176969523 18.528228540433034 12 23.42104457395141 25.046183635173342 31.786734397804768 19.746037393070488 13 29.012945998317413 22.41455480725349 25.614384507376986 22.958114687052106 14 20.32493890810262 23.15951546397765 27.36625394399958 29.149291683920147 15 22.349673393211244 32.14875189548583 23.22713752478712 22.274437186515804 16 21.34668126657617 28.717983112900946 28.674387510953576 21.26094810956931 17 20.968809597405855 29.641526160162833 28.136828353638975 21.252835888792333 18 20.840040358796703 28.281776720206224 30.35730949131586 20.520873429681213 19 23.214080026128123 27.23871511450738 27.55161106421074 21.99559379515376 20 23.116122278179066 28.11622434190766 28.32253887899033 20.445114500922948 21 22.272141340612677 27.163534448566296 27.253068057208186 23.31125615361284 22 22.583152050296565 28.971410741676046 27.258796358398023 21.186640849629367 23 21.590841159377185 28.560576568380014 27.9508231379702 21.897759134272604 24 22.779258498918136 27.359546957652668 28.716691064544538 21.14450347888466 25 22.135206305972652 28.02810042197292 28.278309658718097 21.558383613336325 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 210.0 2 215.5 3 221.0 4 221.0 5 221.0 6 392.0 7 563.0 8 563.0 9 563.0 10 880.0 11 1197.0 12 1197.0 13 1197.0 14 2076.0 15 2955.0 16 2955.0 17 2955.0 18 5424.0 19 7893.0 20 7893.0 21 7893.0 22 12580.0 23 17267.0 24 17267.0 25 17267.0 26 25243.5 27 33220.0 28 33220.0 29 33220.0 30 44170.5 31 55121.0 32 55121.0 33 55121.0 34 62134.0 35 69147.0 36 69147.0 37 69147.0 38 74331.0 39 79515.0 40 79515.0 41 79515.0 42 83576.0 43 87637.0 44 87637.0 45 87637.0 46 89783.0 47 91929.0 48 91929.0 49 91929.0 50 87402.5 51 82876.0 52 82876.0 53 82876.0 54 72435.5 55 61995.0 56 61995.0 57 61995.0 58 52540.5 59 43086.0 60 43086.0 61 43086.0 62 34773.5 63 26461.0 64 26461.0 65 26461.0 66 20391.0 67 14321.0 68 14321.0 69 14321.0 70 10390.5 71 6460.0 72 6460.0 73 6460.0 74 4499.5 75 2539.0 76 2539.0 77 2539.0 78 1709.0 79 879.0 80 879.0 81 879.0 82 572.0 83 265.0 84 265.0 85 265.0 86 169.0 87 73.0 88 73.0 89 73.0 90 46.0 91 19.0 92 19.0 93 19.0 94 11.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11941351778517816 2 0.11256072738724972 3 0.018371310428488948 4 0.0 5 0.006269574193849402 6 0.3435143442025393 7 0.020704175244805 8 0.011664324081580283 9 0.011664324081580283 10 0.004374121530592606 11 0.005686357989770388 12 4.3741215305926056E-4 13 4.3741215305926056E-4 14 0.0 15 0.0017496486122370422 16 4.3741215305926056E-4 17 0.0 18 5.832162040790141E-4 19 1.4580405101975353E-4 20 8.748243061185211E-4 21 0.011081107877501268 22 0.0 23 1.4580405101975353E-4 24 0.0 25 0.003790905326513592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 685852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.3760145579841 #Duplication Level Percentage of deduplicated Percentage of total 1 92.10948258858774 75.87612078645887 2 5.940514800216936 9.787118673291808 3 0.956878415495053 2.3647149085512376 4 0.35114001358206537 1.1570205948290782 5 0.1681366289888764 0.6925212698659027 6 0.09988190534024946 0.49367239730325485 7 0.06248810986039842 0.3603265013292763 8 0.046648398974373964 0.3074167354415744 9 0.043224219476410114 0.32045750395616335 >10 0.18560594579233516 2.8449032699741457 >50 0.018975038619575047 1.0963474252400314 >100 0.014363945214782592 2.538716937893635 >500 0.0015959939107224647 0.9392916681368237 >1k 0.0010639959404816432 1.2213713277281466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1620 0.2362025626520007 No Hit CTGTAGGACGTGGAATATGGCAAGA 1602 0.23357808973364516 No Hit GTATCAACGCAGAGTACTTTTTTTT 1470 0.21433195499903768 No Hit CTTTAGGACGTGAAATATGGCGAGG 1270 0.18517114479508698 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1212 0.17671450983594128 No Hit GTCCTACAGTGTGCATTTCTCATTT 1187 0.17306940856044745 No Hit CTGTAGGACCTGGAATATGGCGAGA 991 0.14449181456057575 No Hit GGTATCAACGCAGAGTACTTTTTTT 898 0.13093203781573867 No Hit GTACATGGGAAGCAGTGGTATCAAC 810 0.11810128132600035 No Hit TATCAACGCAGAGTACTTTTTTTTT 782 0.11401876789744725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4580405101975353E-4 2 0.0 0.0 0.0 0.0 1.4580405101975353E-4 3 0.0 0.0 0.0 0.0 1.4580405101975353E-4 4 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 5 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 6 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 7 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 8 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 9 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 10 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 11 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 12 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 13 0.0 0.0 0.0 1.4580405101975353E-4 1.4580405101975353E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAA 90 5.129059E-7 12.718618 1 TAGGACC 400 0.0 12.37978 4 ACTATAC 135 3.5470293E-10 11.992686 3 TGTAGGA 935 0.0 11.936457 2 GACAGGT 80 2.899712E-5 11.859369 7 GTAAGAC 65 7.848227E-4 11.721359 3 TAGACTG 65 7.8530493E-4 11.720501 5 CTGTAGG 915 0.0 11.676109 1 AATCCCG 140 6.91216E-10 11.520531 19 TATTAGG 75 1.9933209E-4 11.446756 2 GTAGGAC 915 0.0 11.240975 3 CAGGACT 85 5.1837706E-5 11.20342 4 TGGCGAG 720 0.0 11.068745 18 AATGTCC 155 3.1832315E-10 11.016933 8 GGCGAGA 475 0.0 10.785785 19 GGCGAGG 265 0.0 10.7405615 19 GTTCTAA 80 3.6250817E-4 10.731334 1 GTCCTAA 400 0.0 10.731334 1 GTCCAAG 80 3.6250817E-4 10.731334 1 TTAGACT 80 3.6819858E-4 10.71327 4 >>END_MODULE