FastQCFastQC Report
Thu 26 May 2016
SRR1926887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623536
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7160.11482897539195813No Hit
GTCCTACAGTGGACATTTCTAAATT6330.10151779528367248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC551.0864962E-515.6043293
GTAGGAC3350.012.8095253
ATTTAGA751.4130768E-512.7197691
CTTATAC751.4189538E-512.7146393
TCCAACG604.155832E-412.64121418
TAGGACC1650.012.1210574
CAGTACC907.2368475E-611.6400614
TAGACAG907.275905E-611.6344385
CTGTAGG3950.011.5927011
GTTCTAA751.9910833E-411.4477921
TAGACTG1001.8773753E-611.4229025
TCCTATA855.1100495E-511.21882
TTTATAG855.1100495E-511.21883
GTCCTAC4000.011.2092971
TGTAGGA4000.011.2047772
TAATAGT855.177672E-511.2043374
CCAGTAC600.005706059711.1253093
AGGACCT2400.011.1055995
CTGTGCG600.00594327711.0646139
TATGGCG3200.010.96230416
Produced by FastQC (version 0.11.5)