##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926887_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 623536 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.549839945087374 31.0 28.0 31.0 16.0 34.0 2 27.92449353365323 31.0 28.0 31.0 16.0 34.0 3 27.510697056785816 31.0 27.0 31.0 15.0 34.0 4 31.37607932821842 35.0 32.0 37.0 17.0 37.0 5 31.54097277462729 35.0 32.0 37.0 17.0 37.0 6 30.481665854096637 35.0 30.0 37.0 11.0 37.0 7 30.25217950527315 35.0 30.0 37.0 11.0 37.0 8 30.403569320776988 35.0 30.0 37.0 10.0 37.0 9 32.658030971748225 37.0 32.0 39.0 17.0 39.0 10 32.835839470375404 37.0 33.0 39.0 17.0 39.0 11 32.98989633317082 37.0 33.0 39.0 17.0 39.0 12 32.87256068615124 37.0 33.0 39.0 15.0 39.0 13 32.97456762720998 37.0 33.0 39.0 17.0 39.0 14 33.96060532190603 39.0 33.0 40.0 16.0 41.0 15 33.516576428626415 38.0 33.0 40.0 10.0 41.0 16 33.60366843293731 38.0 33.0 40.0 10.0 41.0 17 33.829963306048086 39.0 33.0 40.0 11.0 41.0 18 33.8668416899746 39.0 33.0 40.0 10.0 41.0 19 33.226556285443024 39.0 32.0 40.0 9.0 41.0 20 33.50815349875548 39.0 33.0 40.0 8.0 41.0 21 33.49652626311873 39.0 33.0 40.0 2.0 41.0 22 33.51109158091914 39.0 33.0 40.0 2.0 41.0 23 33.490978868902516 39.0 33.0 40.0 2.0 41.0 24 32.14603647584101 38.0 31.0 40.0 2.0 41.0 25 32.721058607682636 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17543394873820262 1101 2 -0.03537300970742763 1101 3 -0.8931082563884338 1101 4 -0.12459106833044231 1101 5 0.2779387452557316 1101 6 -2.2986346319042497 1101 7 -0.6215422264305523 1101 8 0.020709654248822318 1101 9 0.14680545929657285 1101 10 -8.647760472388203 1101 11 -3.6738843304488995 1101 12 -1.0850540432145905 1101 13 -0.2315781880233274 1101 14 -0.0891704520545602 1101 15 -1.8379590146064722 1101 16 -0.0023139785921415523 1101 17 0.11146008372877247 1101 18 0.3777358592214455 1101 19 -0.5806838279499758 1101 20 0.046224550564403444 1101 21 0.22973962243241175 1101 22 0.47885605917507945 1101 23 0.5998643458471307 1101 24 0.006338898388897007 1101 25 0.1566595246337812 1102 1 -0.43967228048567364 1102 2 -0.37419653911919326 1102 3 -0.5502552429471841 1102 4 -0.3957458594852348 1102 5 -0.16558199473559654 1102 6 -1.118695334317895 1102 7 -0.3228863513040885 1102 8 0.19043938397855342 1102 9 0.048473330376211266 1102 10 -0.10232327048041157 1102 11 -0.29445377690069563 1102 12 -0.2895171159129575 1102 13 -0.27565392151083046 1102 14 -0.3916765945607068 1102 15 0.016256912550630886 1102 16 0.05556721811258569 1102 17 -0.12431387204518529 1102 18 -0.669797021252613 1102 19 0.11890281751902165 1102 20 -0.027121308663808463 1102 21 -0.11200340401649811 1102 22 -0.4028840766827031 1102 23 -0.4656306678831754 1102 24 -4.133964613679325 1102 25 -2.0172326563172227 1103 1 -0.03211525433709994 1103 2 -0.1704695618710872 1103 3 0.10945603929931025 1103 4 -0.0881683361330623 1103 5 0.05615323995738564 1103 6 0.5331450217478135 1103 7 0.09948488233474961 1103 8 0.30434995760743533 1103 9 0.13342662603373157 1103 10 0.1592116772517187 1103 11 0.15663345334146328 1103 12 -6.380561458030343E-5 1103 13 -0.13225431388586628 1103 14 -0.016778055718503992 1103 15 0.26429415889774077 1103 16 0.3288324226453909 1103 17 0.06778826259215975 1103 18 -0.16675113679827547 1103 19 -2.1653629433664534 1103 20 -0.6708002638920618 1103 21 -0.5392037788225039 1103 22 -0.17849123313492754 1103 23 -0.11356952765301997 1103 24 1.4607951298128157 1103 25 0.8173092311824135 1104 1 -0.1555120993621415 1104 2 0.016798225278712664 1104 3 -1.0954936750966127 1104 4 -0.18046922159079415 1104 5 0.027167403877676577 1104 6 -1.6576793445060929 1104 7 -0.39485465468600367 1104 8 0.2259452563430635 1104 9 0.26713471427319746 1104 10 0.4453716495705251 1104 11 0.2518261155572077 1104 12 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1108 21 0.5241628462674015 1108 22 0.9242724075326052 1108 23 0.9573127797661556 1108 24 0.16802721007720578 1108 25 0.7292913046185063 1109 1 0.4903950067185434 1109 2 0.613064871669188 1109 3 -0.7113688461769705 1109 4 0.4238246238611829 1109 5 0.5637731695573294 1109 6 0.9926649886606853 1109 7 1.2527893243715873 1109 8 1.63938185341895 1109 9 0.8985439819406338 1109 10 0.9970161934648587 1109 11 0.6242251509898509 1109 12 0.5925104718407681 1109 13 0.47281305168643684 1109 14 0.8353863189752389 1109 15 -0.5577040608709183 1109 16 0.5505217992497364 1109 17 0.437355972072794 1109 18 0.6185107055613415 1109 19 -0.8521995711767261 1109 20 0.29108997315743323 1109 21 0.3449114776701734 1109 22 0.6663746426175123 1109 23 0.5861614146557983 1109 24 -0.14774391164949208 1109 25 0.17674850326559977 1110 1 0.4009652801518868 1110 2 0.7991367942141387 1110 3 0.9945264618345213 1110 4 0.6633809596415823 1110 5 0.9805302713766721 1110 6 0.9056982900757298 1110 7 0.9066354782177406 1110 8 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-0.2823371748983021 2215 1 0.20514110432771204 2215 2 -0.28943463435728845 2215 3 -0.06225009494203704 2215 4 0.19817949444011873 2215 5 0.3036071944535941 2215 6 1.2712660556434976 2215 7 0.7651702767525386 2215 8 -0.6564332028940321 2215 9 0.11205119395407337 2215 10 -0.3454635536206965 2215 11 -0.38930577175269576 2215 12 -0.6769817233775655 2215 13 -0.2974299832868894 2215 14 -0.20259036547447806 2215 15 -0.25102751473379215 2215 16 -0.23822943568406885 2215 17 -0.6004013881327026 2215 18 -1.054996506452099 2215 19 -0.055305676689471284 2215 20 -3.846426327968828 2215 21 -2.363792335805428 2215 22 -1.2573242311228583 2215 23 -1.2732754555279655 2215 24 -1.1803481400628506 2215 25 -0.6649804040649698 2216 1 0.03827074924926421 2216 2 -0.786296731797421 2216 3 0.29067586156773473 2216 4 -0.1558750059727636 2216 5 -0.24013076315719672 2216 6 -1.4314589650005978 2216 7 -9.181700078325907 2216 8 -10.423606338044046 2216 9 -5.801981286089969 2216 10 -3.175460801074589 2216 11 -1.9962587032142949 2216 12 -1.7826244428931197 2216 13 -1.5027423972698237 2216 14 -1.708893696055263 2216 15 -2.5386740878138916 2216 16 -1.5649951940105211 2216 17 -1.9740540168142289 2216 18 -1.6183436025159565 2216 19 -1.125011154256221 2216 20 -1.9818075556043908 2216 21 -4.479261644916356 2216 22 -3.029545535829712 2216 23 -2.903311238627328 2216 24 -3.313081418345263 2216 25 -2.857735702716223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39780.0 3 1.0 4 326.0 5 1885.0 6 2188.0 7 1795.0 8 1407.0 9 1322.0 10 1251.0 11 1198.0 12 1140.0 13 1209.0 14 1233.0 15 1273.0 16 1365.0 17 1477.0 18 1812.0 19 2176.0 20 2852.0 21 3597.0 22 4608.0 23 5344.0 24 6277.0 25 7265.0 26 8287.0 27 8930.0 28 10150.0 29 11910.0 30 14020.0 31 17179.0 32 21668.0 33 28616.0 34 38244.0 35 52323.0 36 74606.0 37 113580.0 38 131242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12711421799044 17.796472620625927 12.314009079586999 25.762404081796635 2 14.904563329260126 19.672944542840124 41.009908971063105 24.412583156836646 3 19.446616705553264 23.372905061573565 28.72062830890677 28.459849923966402 4 11.99055737789104 16.461841150831606 37.74818176566121 33.799419705616145 5 13.222435213628597 37.348871903047026 36.105839796597664 13.322853086726713 6 32.45421821261742 36.26899286484267 17.870938350187362 13.405850572352549 7 28.331805388017695 30.86787293928428 21.611258544431042 19.18906312826699 8 25.05837814888197 36.49245426240898 20.979466330442836 17.46970125826622 9 25.949012770066616 15.768570012399524 19.6167536344402 38.665663583093654 10 14.599514504693708 29.235288738145393 34.16388434750939 22.001312409651504 11 34.98505844290208 22.691597599726673 23.307545847241627 19.015798110129623 12 22.996856052099705 24.646947483237625 31.254050239004204 21.102146225658462 13 28.880662013919746 21.572234938589833 25.394309875511485 24.152793171978935 14 20.950495811825697 21.606231413071725 27.155602465103897 30.28767030999868 15 23.682218165836318 30.008308818575824 22.92624399695878 23.383229018629077 16 22.616488720067757 28.096206657598234 26.901323050073145 22.385981572260864 17 22.211724472020197 28.236773636780693 27.06548136551313 22.486020525685984 18 21.903451196273554 27.949139927755212 28.236579215829693 21.910829660141538 19 24.024479254393352 26.847614235183713 26.652710604201257 22.475195906221675 20 23.506068120791372 27.279274712794866 27.39717238082711 21.81748478558665 21 23.440454899067266 26.659555527039707 26.592507639128378 23.307481934764652 22 22.966699817137723 27.49334317153765 27.009720573610085 22.530236437714542 23 22.859718548437545 27.235323085412354 26.906487052682742 22.99847131346736 24 23.4446053828275 26.92392212720976 27.120737472069706 22.510735017893033 25 23.038784011600395 26.937716845757404 27.232218046379124 22.791281096263074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 139.0 2 183.5 3 228.0 4 228.0 5 228.0 6 365.0 7 502.0 8 502.0 9 502.0 10 785.5 11 1069.0 12 1069.0 13 1069.0 14 1765.5 15 2462.0 16 2462.0 17 2462.0 18 4367.0 19 6272.0 20 6272.0 21 6272.0 22 9899.0 23 13526.0 24 13526.0 25 13526.0 26 19436.5 27 25347.0 28 25347.0 29 25347.0 30 32997.5 31 40648.0 32 40648.0 33 40648.0 34 47530.5 35 54413.0 36 54413.0 37 54413.0 38 60592.0 39 66771.0 40 66771.0 41 66771.0 42 71592.5 43 76414.0 44 76414.0 45 76414.0 46 79830.0 47 83246.0 48 83246.0 49 83246.0 50 81386.5 51 79527.0 52 79527.0 53 79527.0 54 72338.5 55 65150.0 56 65150.0 57 65150.0 58 56615.5 59 48081.0 60 48081.0 61 48081.0 62 39200.0 63 30319.0 64 30319.0 65 30319.0 66 23586.0 67 16853.0 68 16853.0 69 16853.0 70 12291.0 71 7729.0 72 7729.0 73 7729.0 74 5425.5 75 3122.0 76 3122.0 77 3122.0 78 2148.5 79 1175.0 80 1175.0 81 1175.0 82 786.5 83 398.0 84 398.0 85 398.0 86 253.0 87 108.0 88 108.0 89 108.0 90 66.0 91 24.0 92 24.0 93 24.0 94 14.0 95 4.0 96 4.0 97 4.0 98 6.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2022337122475687 2 0.12300813425367581 3 0.1258948962048703 4 0.0647917682379205 5 0.022612968617690077 6 0.023094095609555824 7 0.2864309358240743 8 0.27712914731466987 9 0.020046957994406096 10 0.04089579430858844 11 0.01716019604321162 12 0.018603577018808857 13 0.016839444715301122 14 0.01828282569089836 15 0.016358317723435375 16 0.020528084986271844 17 0.019565831002540352 18 0.01603756639552488 19 0.024216725257242565 20 0.01828282569089836 21 0.016197942059480126 22 0.018603577018808857 23 0.020046957994406096 24 0.017480947371122116 25 0.017480947371122116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 623536.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.91171987523082 #Duplication Level Percentage of deduplicated Percentage of total 1 94.15233363164562 83.71245913456147 2 4.435057780292551 7.8865722998366845 3 0.7401845569811386 1.9743324595883645 4 0.25317806343926724 0.9004198822026218 5 0.12791710607724258 0.5686664951394989 6 0.07209679981826642 0.3846150281605378 7 0.042227473539310854 0.26281621088561496 8 0.027788924043197275 0.1976608824130258 9 0.023357061373140704 0.18690448481255342 >10 0.10908412722284318 1.6984927108210826 >50 0.00883820681923719 0.5879202592642541 >100 0.007395159515651144 1.3370033705643085 >500 5.411092325677219E-4 0.30213678175000397 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 716 0.11482897539195813 No Hit GTCCTACAGTGGACATTTCTAAATT 633 0.10151779528367248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.6037566395524878E-4 0.0 6 0.0 0.0 0.0 1.6037566395524878E-4 0.0 7 0.0 0.0 0.0 1.6037566395524878E-4 0.0 8 0.0 0.0 0.0 1.6037566395524878E-4 0.0 9 0.0 0.0 0.0 3.2075132791049756E-4 0.0 10 0.0 0.0 0.0 3.2075132791049756E-4 0.0 11 0.0 0.0 0.0 3.2075132791049756E-4 0.0 12 0.0 0.0 0.0 3.2075132791049756E-4 0.0 13 0.0 1.6037566395524878E-4 0.0 3.2075132791049756E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTACAC 55 1.0864962E-5 15.604329 3 GTAGGAC 335 0.0 12.809525 3 ATTTAGA 75 1.4130768E-5 12.719769 1 CTTATAC 75 1.4189538E-5 12.714639 3 TCCAACG 60 4.155832E-4 12.641214 18 TAGGACC 165 0.0 12.121057 4 CAGTACC 90 7.2368475E-6 11.640061 4 TAGACAG 90 7.275905E-6 11.634438 5 CTGTAGG 395 0.0 11.592701 1 GTTCTAA 75 1.9910833E-4 11.447792 1 TAGACTG 100 1.8773753E-6 11.422902 5 TCCTATA 85 5.1100495E-5 11.2188 2 TTTATAG 85 5.1100495E-5 11.2188 3 GTCCTAC 400 0.0 11.209297 1 TGTAGGA 400 0.0 11.204777 2 TAATAGT 85 5.177672E-5 11.204337 4 CCAGTAC 60 0.0057060597 11.125309 3 AGGACCT 240 0.0 11.105599 5 CTGTGCG 60 0.005943277 11.064613 9 TATGGCG 320 0.0 10.962304 16 >>END_MODULE