FastQCFastQC Report
Thu 26 May 2016
SRR1926887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623536
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13350.21410151138025713No Hit
GGTATCAACGCAGAGTACTTTTTTT8340.13375330373867747No Hit
GTCCTACAGTGGACATTTCTAAATT6680.10713094352210617No Hit
TATCAACGCAGAGTACTTTTTTTTT6510.10440455723486695No Hit
CTGTAGGACGTGGAATATGGCAAGA6500.10424418157091171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA250.006070610618.97690614
CTAGACC500.001473627313.3287263
TAGGACC1550.012.8977294
CTCTAAA603.9742113E-412.7083411
CTACACT1600.012.4946754
GATATAC1404.9112714E-1112.2544711
CTTAAAC701.06715874E-412.2406663
GTCTAGA802.7653286E-511.9140691
AGGACGT2900.011.4893575
GTCCTAT752.007793E-411.4375061
GCTATAC752.0289324E-411.4246213
TCCAACG752.0904189E-411.38796918
TACACTG3300.011.2506175
TTAGGCT855.199542E-511.1997084
TCTACAC1451.1659722E-911.1619873
CCCGTTT600.005922343611.06986112
GTCCTAC3450.011.050731
CAATACT700.00146363910.8797174
GACGTGA1752.0008883E-1110.8448157
CACTGTT1752.0008883E-1110.8448157
Produced by FastQC (version 0.11.5)