##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 623536 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0738978984373 31.0 30.0 33.0 26.0 34.0 2 30.346417849169896 31.0 30.0 34.0 26.0 34.0 3 30.41374355289831 31.0 30.0 34.0 26.0 34.0 4 34.057606938492725 37.0 35.0 37.0 30.0 37.0 5 34.08277148392394 37.0 35.0 37.0 30.0 37.0 6 34.05189756485592 37.0 35.0 37.0 30.0 37.0 7 34.0450848066511 37.0 35.0 37.0 30.0 37.0 8 34.072149803700185 37.0 35.0 37.0 30.0 37.0 9 35.64367253855431 39.0 35.0 39.0 30.0 39.0 10 35.53111127505068 39.0 35.0 39.0 30.0 39.0 11 35.57922397423725 39.0 35.0 39.0 30.0 39.0 12 35.47737099381592 39.0 35.0 39.0 30.0 39.0 13 35.503340625080185 39.0 35.0 39.0 30.0 39.0 14 36.62215493572143 40.0 37.0 41.0 30.0 41.0 15 36.60473493110261 40.0 37.0 41.0 30.0 41.0 16 36.5883974622155 40.0 37.0 41.0 30.0 41.0 17 36.55967899207103 40.0 37.0 41.0 30.0 41.0 18 36.52888526083498 40.0 37.0 41.0 30.0 41.0 19 36.6060115213877 40.0 37.0 41.0 30.0 41.0 20 36.54759949706192 40.0 37.0 41.0 30.0 41.0 21 36.50434938800647 40.0 37.0 41.0 30.0 41.0 22 36.4233003387134 40.0 37.0 41.0 30.0 41.0 23 36.360436285956226 40.0 37.0 41.0 30.0 41.0 24 36.29598932539581 40.0 37.0 41.0 30.0 41.0 25 36.184189846295965 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2690734826726562 1101 2 0.3621024292761348 1101 3 0.39413704036718045 1101 4 0.3621529895504594 1101 5 0.112844984962436 1101 6 0.3506500376635131 1101 7 0.20076285507879277 1101 8 0.26193486240938313 1101 9 0.24275163042607062 1101 10 0.4084949055661298 1101 11 0.3734485378187955 1101 12 0.47819256355735007 1101 13 0.39809251007103796 1101 14 0.35343719191019574 1101 15 0.21886167628006348 1101 16 0.047425754461556835 1101 17 0.19315112750761187 1101 18 -0.03471886377064237 1101 19 -0.056554625138460324 1101 20 -0.039294044584920584 1101 21 0.020439882709304413 1101 22 -0.1577948168915171 1101 23 0.07950254287183611 1101 24 0.007030377131869159 1101 25 0.22483283187355596 1102 1 -0.47111440575052654 1102 2 -0.5437567989360339 1102 3 -0.3603766176758896 1102 4 -0.30683963944217396 1102 5 -0.43114692138241395 1102 6 -0.272249225235754 1102 7 -0.2972224029064634 1102 8 -0.5408516674359731 1102 9 -0.6297559573756359 1102 10 -0.3887937199577891 1102 11 -0.365392335139731 1102 12 -0.3147717376422463 1102 13 -0.34873217557718306 1102 14 -0.42834630281447517 1102 15 -0.2552819860988933 1102 16 -0.33808082163325537 1102 17 -0.21264739829091894 1102 18 -0.3566208727314759 1102 19 -0.180260362961846 1102 20 -0.11495237671383762 1102 21 -0.062335084771547145 1102 22 -0.29506320827755417 1102 23 -0.2972628856582915 1102 24 -0.11270946966679674 1102 25 -0.3770980829985362 1103 1 -0.5619217027550043 1103 2 -0.5040988094188883 1103 3 -0.2573365895654405 1103 4 -0.38647303231500274 1103 5 -0.4796046532204272 1103 6 -0.4580370498968165 1103 7 -0.3931305754343555 1103 8 -0.4660099351294491 1103 9 -0.4975337676886511 1103 10 -0.2985369192784546 1103 11 -0.30208057267303445 1103 12 -0.060908600398825286 1103 13 -0.010331543092043205 1103 14 -0.29572822428090006 1103 15 -0.19273037706841478 1103 16 -0.21401769024618034 1103 17 -0.327798345554541 1103 18 -0.3821849850561492 1103 19 -0.21615740843635223 1103 20 -0.4065537939490582 1103 21 -0.17292979649302254 1103 22 -0.23294965376963717 1103 23 -0.44445154777653784 1103 24 -0.5218008579131137 1103 25 -0.40999199532906516 1104 1 -0.2844892465599216 1104 2 -0.3480132019358919 1104 3 -0.15366372677451778 1104 4 -0.06859427365753845 1104 5 0.022148677881773438 1104 6 -0.10865505514943408 1104 7 -0.11204057186076 1104 8 -0.21748390176453825 1104 9 -0.21783576493187695 1104 10 -0.06377320205769621 1104 11 -0.12827333131914997 1104 12 0.015348539703161634 1104 13 -0.03894867479891673 1104 14 -0.13316022668598748 1104 15 -0.2678757316769307 1104 16 -0.07803271069509066 1104 17 -0.09823392722822888 1104 18 -0.12003957939625565 1104 19 -0.16351209637064557 1104 20 -0.2162909928168517 1104 21 -0.16580756248486495 1104 22 -0.11387175504422231 1104 23 -0.26707751704361016 1104 24 -0.1714840557712236 1104 25 -0.31907063146323367 1105 1 -0.19967785148899608 1105 2 -0.2804123080787484 1105 3 -0.20761775850966302 1105 4 -0.14098276926596043 1105 5 -0.3166242513223807 1105 6 -0.5132561687196642 1105 7 -0.8503397782142201 1105 8 -0.6135762241318687 1105 9 -0.6359669175681688 1105 10 -0.3785406009226904 1105 11 -0.498816772883508 1105 12 -0.4685154096663666 1105 13 -0.5042474042142118 1105 14 -0.49750561565853957 1105 15 -0.5788372711690215 1105 16 -0.6761228144961251 1105 17 -0.3882948574184155 1105 18 -0.4564288895507502 1105 19 -0.34151184986328076 1105 20 -0.4702739623731915 1105 21 -0.646724141176982 1105 22 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0.40555176341153754 1107 10 0.4302955062547227 1107 11 0.33825246877904647 1107 12 0.3345558618223379 1107 13 0.4614623392748669 1107 14 0.4242587152699926 1107 15 0.308657295648608 1107 16 0.4426112297437257 1107 17 0.36629031708515924 1107 18 0.209393993557363 1107 19 0.451558051870272 1107 20 0.5644757553170336 1107 21 0.42759855803880953 1107 22 0.49738175512631244 1107 23 0.4859421950810301 1107 24 0.623956722341859 1107 25 0.6401126366779408 1108 1 0.795696859296033 1108 2 0.41773650108667937 1108 3 0.6297176362418924 1108 4 0.749643440276202 1108 5 0.7776845571549487 1108 6 0.7524453012234815 1108 7 0.6104076220765009 1108 8 0.31254601596171483 1108 9 0.35810686342836817 1108 10 0.5847936068648281 1108 11 0.49185953858273734 1108 12 0.5263400043518445 1108 13 0.5360986216065555 1108 14 0.4471919665473223 1108 15 0.5483689337284972 1108 16 0.4340949676013608 1108 17 0.43161560420738 1108 18 0.3540320911567818 1108 19 0.4975706613397719 1108 20 0.3425050386847559 1108 21 0.42880504695681765 1108 22 0.3063381090061128 1108 23 0.3613971188840637 1108 24 0.33422671021055805 1108 25 0.3643668272899063 1109 1 0.5059552365058373 1109 2 0.27648767790595485 1109 3 0.4300114052017605 1109 4 0.4170002170117897 1109 5 0.42172287787742846 1109 6 0.4439355902851645 1109 7 0.3948971440263591 1109 8 0.3273652216522862 1109 9 0.3289566141170184 1109 10 0.2989790735826361 1109 11 0.2512915271457956 1109 12 0.5149422917398283 1109 13 0.48248796572595154 1109 14 0.4700702049796277 1109 15 0.346030114383332 1109 16 0.3134230473809865 1109 17 0.4025438095143983 1109 18 0.34681862485739856 1109 19 0.4723257085868866 1109 20 0.4788112209086819 1109 21 0.6078297688787799 1109 22 0.3891164485577008 1109 23 0.40416360307109755 1109 24 0.47757201513751113 1109 25 0.4445212957162781 1110 1 0.6113249566888364 1110 2 0.17305539787616198 1110 3 0.43793246063318847 1110 4 0.6793557867532556 1110 5 0.5895322192967285 1110 6 0.5031908302043036 1110 7 0.4218351161510583 1110 8 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0.35315085886863784 2214 5 0.4965494468608327 2214 6 0.6553552933453304 2214 7 0.5337351562151582 2214 8 0.5603138731045689 2214 9 0.47568006458382683 2214 10 0.4821068290286945 2214 11 0.4230652239818937 2214 12 0.6484841745199148 2214 13 0.49402437003092814 2214 14 0.4787620164422819 2214 15 0.46504107133353756 2214 16 0.5151824807316103 2214 17 0.6140850763712464 2214 18 0.46758415260636355 2214 19 0.6635698735246436 2214 20 0.5118360523402146 2214 21 0.5230228121879108 2214 22 0.47800794881703723 2214 23 0.5463203069092728 2214 24 0.5687083935088779 2214 25 0.4811768325420047 2215 1 0.3802626856265654 2215 2 0.21217627699704167 2215 3 0.17903136173208978 2215 4 0.1315902189876823 2215 5 0.1768582266227341 2215 6 0.17549852251199383 2215 7 0.1329706472865837 2215 8 0.06349839691409187 2215 9 0.09160826398859001 2215 10 0.1500737189096455 2215 11 0.03241976267236879 2215 12 0.04262851975801141 2215 13 0.15747117062616667 2215 14 0.06460093013275525 2215 15 0.11143616061924888 2215 16 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quality scores pass #Quality Count 2 12449.0 3 3.0 4 194.0 5 1293.0 6 1741.0 7 1353.0 8 1065.0 9 954.0 10 805.0 11 774.0 12 744.0 13 674.0 14 629.0 15 681.0 16 664.0 17 816.0 18 887.0 19 978.0 20 1283.0 21 1767.0 22 2105.0 23 2692.0 24 3159.0 25 3805.0 26 4765.0 27 5079.0 28 5961.0 29 7468.0 30 9699.0 31 12434.0 32 16723.0 33 22014.0 34 30097.0 35 42184.0 36 61808.0 37 107779.0 38 256010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55801855437704 18.83664248624799 10.528757999929352 28.076580959445618 2 14.810284072210047 20.283510326614813 39.23696229762163 25.669243303553515 3 19.006808940644323 23.841236997650153 28.84600887006873 28.305945191636795 4 12.402812347643119 16.6829180672808 37.024806907700594 33.889462677375484 5 13.506057758004037 38.027387417441595 34.84726494712092 13.619289877433449 6 32.84778784703949 37.06834813144651 17.188503338628145 12.895360682885848 7 28.96557919288036 31.512892162681545 20.725150063682822 18.796378580755274 8 25.847429082848684 37.285756904056946 19.13022836557739 17.73658564751698 9 26.175158668674786 15.817466465854757 19.231750095032794 38.775624770437666 10 14.796649911949524 29.695784803995473 33.407537377346806 22.100027906708196 11 35.30488225397472 22.669337597413325 23.014965445234424 19.01081470337753 12 23.11084880696161 24.734016108183354 31.06743176795492 21.08770331690012 13 29.167114385652038 21.421068078411498 25.243813058212023 24.168004477724445 14 21.094726037832682 21.742003255631197 26.88798543786636 30.275285268669762 15 23.547044049768893 29.974884607118913 22.875215309163107 23.60285603394908 16 22.839331936991407 28.223320620848263 26.524936891973816 22.412410550186518 17 22.296743566920863 28.319661286054508 26.87964588996608 22.503949257058544 18 22.096273665471866 27.749204134491244 28.244765730873166 21.909756469163717 19 23.625656339509955 26.98409389062986 26.930528247056294 22.45972152280389 20 23.451204667595782 27.24266398089596 27.563737604906237 21.742393746602023 21 23.343192324102024 26.55768311578326 26.970541720532896 23.128582839581817 22 23.03411511123656 27.41750275846142 26.974384798953068 22.573997331348952 23 22.93788971286341 27.063874419440097 27.008544815375537 22.989691052320957 24 22.992614688830617 26.770109135814344 27.63132783243924 22.605948342915795 25 23.081549579950536 26.86616476716846 27.295516585458174 22.75676906742283 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 157.0 2 144.0 3 131.0 4 131.0 5 131.0 6 268.5 7 406.0 8 406.0 9 406.0 10 700.0 11 994.0 12 994.0 13 994.0 14 1626.5 15 2259.0 16 2259.0 17 2259.0 18 4030.0 19 5801.0 20 5801.0 21 5801.0 22 9394.5 23 12988.0 24 12988.0 25 12988.0 26 18786.0 27 24584.0 28 24584.0 29 24584.0 30 32485.5 31 40387.0 32 40387.0 33 40387.0 34 46933.5 35 53480.0 36 53480.0 37 53480.0 38 59572.0 39 65664.0 40 65664.0 41 65664.0 42 70933.5 43 76203.0 44 76203.0 45 76203.0 46 79931.0 47 83659.0 48 83659.0 49 83659.0 50 82378.5 51 81098.0 52 81098.0 53 81098.0 54 73647.5 55 66197.0 56 66197.0 57 66197.0 58 57296.5 59 48396.0 60 48396.0 61 48396.0 62 39754.5 63 31113.0 64 31113.0 65 31113.0 66 24173.0 67 17233.0 68 17233.0 69 17233.0 70 12569.0 71 7905.0 72 7905.0 73 7905.0 74 5541.0 75 3177.0 76 3177.0 77 3177.0 78 2186.5 79 1196.0 80 1196.0 81 1196.0 82 787.0 83 378.0 84 378.0 85 378.0 86 242.0 87 106.0 88 106.0 89 106.0 90 63.5 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11514972671986862 2 0.10119704395576197 3 0.014594185419927639 4 0.0 5 0.006094275230299453 6 0.3484963177747556 7 0.021490338970003336 8 0.009943291165225424 9 0.010745169485001668 10 0.004811269918657463 11 0.005292396910523209 12 3.2075132791049756E-4 13 8.018783197762439E-4 14 1.6037566395524878E-4 15 0.002245259295373483 16 3.2075132791049756E-4 17 1.6037566395524878E-4 18 1.6037566395524878E-4 19 3.2075132791049756E-4 20 4.811269918657463E-4 21 0.012509301788509404 22 0.0 23 0.0 24 1.6037566395524878E-4 25 0.006094275230299453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 623536.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.98806538158738 #Duplication Level Percentage of deduplicated Percentage of total 1 92.31828185958503 79.38270456457809 2 5.84646544996858 10.054525067261798 3 0.9730738196363455 2.5101820567200317 4 0.3331303343200587 1.1458093187241307 5 0.17020986311516406 0.7318008419068883 6 0.08661807964958082 0.44688726576814203 7 0.05048274093378737 0.30386392596393724 8 0.038386016654276114 0.26405914478452774 9 0.028362226677301202 0.219493170170566 >10 0.13594520542637598 2.0843710807855658 >50 0.00840199307246675 0.5232283657083711 >100 0.009708866140136881 1.6687299480701985 >500 7.468358566903807E-4 0.450014435599402 >1k 1.8670896417259517E-4 0.21433081395833092 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1335 0.21410151138025713 No Hit GGTATCAACGCAGAGTACTTTTTTT 834 0.13375330373867747 No Hit GTCCTACAGTGGACATTTCTAAATT 668 0.10713094352210617 No Hit TATCAACGCAGAGTACTTTTTTTTT 651 0.10440455723486695 No Hit CTGTAGGACGTGGAATATGGCAAGA 650 0.10424418157091171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGGTA 25 0.0060706106 18.976906 14 CTAGACC 50 0.0014736273 13.328726 3 TAGGACC 155 0.0 12.897729 4 CTCTAAA 60 3.9742113E-4 12.708341 1 CTACACT 160 0.0 12.494675 4 GATATAC 140 4.9112714E-11 12.254471 1 CTTAAAC 70 1.06715874E-4 12.240666 3 GTCTAGA 80 2.7653286E-5 11.914069 1 AGGACGT 290 0.0 11.489357 5 GTCCTAT 75 2.007793E-4 11.437506 1 GCTATAC 75 2.0289324E-4 11.424621 3 TCCAACG 75 2.0904189E-4 11.387969 18 TACACTG 330 0.0 11.250617 5 TTAGGCT 85 5.199542E-5 11.199708 4 TCTACAC 145 1.1659722E-9 11.161987 3 CCCGTTT 60 0.0059223436 11.069861 12 GTCCTAC 345 0.0 11.05073 1 CAATACT 70 0.001463639 10.879717 4 GACGTGA 175 2.0008883E-11 10.844815 7 CACTGTT 175 2.0008883E-11 10.844815 7 >>END_MODULE