FastQCFastQC Report
Thu 26 May 2016
SRR1926886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15250.24622230402722817No Hit
CTGTAGGACGTGGAATATGGCAAGA12610.20359759041202277No Hit
GTCCTAAAGTGTGTATTTCTCATTT11580.18696749381215094No Hit
GTCCTACAGTGTGCATTTCTCATTT11080.1788946313850287No Hit
CTTTAGGACGTGAAATATGGCGAGG9610.15516041584928936No Hit
CTGTAGGACCTGGAATATGGCGAGA7340.11850962043015441No Hit
GTATCAACGCAGAGTACTTTTTTTT6630.10704615578364084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT307.52768E-419.0697481
CCAACGA453.584627E-516.86053319
TAGGACC2900.014.4432494
CTAATAC759.3315975E-713.974273
CTCCTAT706.976412E-613.6212481
GTCCTAA4150.013.0960911
TAGGACT957.1646355E-813.0265684
GTGTTAG603.961425E-412.7131651
CTAGGAC905.198963E-712.703883
CTAAACT604.016045E-412.6925534
GTTCAAG701.0522222E-412.2591231
TCCAACG701.1060707E-412.19377818
AGGACCT4650.012.076445
GGCGAGG2800.011.85506219
GTTCTAT657.7688263E-411.73522951
GAACTAG657.7688263E-411.73522951
TCCTAAA4700.011.5551262
GGACCTG4700.011.5429516
TGTAGGA8650.011.4555232
GTCCTAG1251.726221E-811.4418491
Produced by FastQC (version 0.11.5)