FastQCFastQC Report
Thu 26 May 2016
SRR1926886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16210.26172219988730283No Hit
CTGTAGGACGTGGAATATGGCAAGA15360.24799833376119504No Hit
GTCCTAAAGTGTGTATTTCTCATTT12090.19520181348781562No Hit
GTCCTACAGTGTGCATTTCTCATTT11650.18809769455194805No Hit
CTTTAGGACGTGAAATATGGCGAGG11250.18163940461025027No Hit
GTATCAACGCAGAGTACTTTTTTTT10980.17728005889960427No Hit
CTGTAGGACCTGGAATATGGCGAGA9100.14692609617362468No Hit
GGTATCAACGCAGAGTACTTTTTTT6780.1094680145117775No Hit
GATATACACTGTTCTACAAATCCCG6470.10446283980696171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA651.9321487E-716.05902119
CCGGTTT551.1406908E-515.524384512
CCAACGT508.783521E-515.18307519
TCCAACG1203.6379788E-1214.23413318
CGGTTTC602.596335E-514.23068513
TGCCGGT602.596335E-514.23068510
TAGGACC3350.013.9272834
TACACTA852.6163616E-713.442425
TTTAGTA500.001458777213.34662152
CTAGGAC1457.2759576E-1212.4787513
GCCGGTT701.1027315E-412.1977311
GTGTAGA1800.012.1804521
AGTGCTC550.003094281812.0754968
CTGTAGG8450.012.0707741
GTCCTAC8600.011.9710811
CCCGTTT802.8998114E-511.85890512
TGTAGGA8750.011.7668162
AGCACCG657.85941E-411.7190335
CGTTTCC907.5533753E-611.59537314
GTAGGAC8700.011.4935873
Produced by FastQC (version 0.11.5)