FastQCFastQC Report
Thu 26 May 2016
SRR1926885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392378
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5840.14883607133937174No Hit
GTACATGGGAAGCAGTGGTATCAAC4680.11927274210072938No Hit
GTCCTACAGTGGACATTTCTAAATT4540.11570475408916912No Hit
CCCATGTACTCTGCGTTGATACCAC3940.10041337689676792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA250.00594258119.0449124
AGTACTG453.468641E-516.9223165
ACTGGAC456.600931E-414.8202963
GTGCAAT400.00516585514.29282951
GTCCTAT655.281294E-513.1933821
GTGTAAA603.9778493E-412.7047371
CTATACT603.9993972E-412.6966094
TAAGGCT603.9993972E-412.6966094
TCTTGGA550.00299620412.1256962
TGTTAGA550.00299620412.1256962
TGTAGGG550.00299620412.1256962
TAGGACC951.0041149E-612.0283674
GTGTAGG657.8009337E-411.727451
GTCTTAG657.8009337E-411.727451
CTACACC657.842956E-411.7199484
ACAGATC657.919095E-411.7064668
CTCTTAG600.00573280611.1166451
TACTGTT600.00577668511.1052697
CTAGCAC700.001451854510.8883813
ATGCCGG700.001508658910.83842819
Produced by FastQC (version 0.11.5)