##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926885_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392378 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.631215307687995 31.0 28.0 31.0 16.0 34.0 2 28.003649542023254 31.0 28.0 31.0 16.0 34.0 3 27.61660184821779 31.0 27.0 31.0 15.0 34.0 4 31.47191993434902 35.0 32.0 37.0 17.0 37.0 5 31.62641891237531 35.0 32.0 37.0 17.0 37.0 6 30.62773651937672 35.0 30.0 37.0 11.0 37.0 7 30.432557380892913 35.0 30.0 37.0 11.0 37.0 8 30.539502724413705 35.0 31.0 37.0 10.0 37.0 9 32.771567213248446 37.0 32.0 39.0 17.0 39.0 10 32.921552686440116 37.0 33.0 39.0 17.0 39.0 11 33.09972526492311 37.0 33.0 39.0 17.0 39.0 12 32.95877699565215 37.0 33.0 39.0 16.0 39.0 13 33.07082711059234 37.0 33.0 39.0 17.0 39.0 14 34.072667172981156 39.0 34.0 40.0 17.0 41.0 15 33.63305537007681 39.0 33.0 40.0 10.0 41.0 16 33.70354861893378 39.0 33.0 40.0 11.0 41.0 17 33.92955007671174 39.0 33.0 40.0 11.0 41.0 18 33.9802155064759 39.0 34.0 40.0 11.0 41.0 19 33.32514310180489 39.0 32.0 40.0 10.0 41.0 20 33.61245533643579 39.0 33.0 40.0 9.0 41.0 21 33.60893067399294 39.0 33.0 40.0 2.0 41.0 22 33.635435727793094 39.0 34.0 40.0 2.0 41.0 23 33.61701726396485 39.0 34.0 40.0 2.0 41.0 24 32.278114471249665 38.0 31.0 40.0 2.0 41.0 25 32.861437185571056 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.13553780815718852 1101 2 -0.26985630212412204 1101 3 -0.9350740880585207 1101 4 -0.31740474145196984 1101 5 -0.029471689601489715 1101 6 -2.598780667054669 1101 7 -1.18313673366994 1101 8 -0.09794142156378882 1101 9 -0.2851166622577992 1101 10 -9.7668528082509 1101 11 -4.236213273516153 1101 12 -1.50346890923824 1101 13 -0.9098517791510901 1101 14 -0.6977161593401178 1101 15 -2.5489144717412806 1101 16 -0.5130043404711344 1101 17 -0.10498577643355844 1101 18 -0.03515604937712169 1101 19 -0.7396800594535833 1101 20 0.14035716841620172 1101 21 -0.041353228440087264 1101 22 0.22685427164437755 1101 23 0.21800665416741083 1101 24 0.43465637527148715 1101 25 0.22819505856893585 1102 1 -0.4045169236145796 1102 2 -0.20074220048955027 1102 3 -0.8786480976195499 1102 4 -0.4279548838063896 1102 5 -0.07108456810803432 1102 6 -1.1998994607851792 1102 7 -0.3239497159213194 1102 8 0.30068926450787714 1102 9 0.29196398166752857 1102 10 0.32789687786203103 1102 11 -0.03980249112683509 1102 12 0.07490559782642947 1102 13 -0.020463814730852903 1102 14 -0.39969143741753754 1102 15 0.18556359153127744 1102 16 0.25690317336642465 1102 17 0.08718679366788251 1102 18 -0.02082924135812192 1102 19 0.25945638268804316 1102 20 0.38390995744283174 1102 21 -0.06281838844881094 1102 22 -0.26756060159240036 1102 23 -0.4008018128258186 1102 24 -3.3990217917459056 1102 25 -1.6328517032364616 1103 1 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-0.19190221380929984 2214 22 -0.08063044936523056 2214 23 -0.0504061828302369 2214 24 -1.1130114290516069 2214 25 -0.27696143533269435 2215 1 0.15588835164946246 2215 2 -0.24771656436179157 2215 3 0.29169963365079354 2215 4 0.24756225602630977 2215 5 0.355672195102013 2215 6 1.0363094804212558 2215 7 0.5962129672365144 2215 8 -0.8307625473718154 2215 9 -0.2944276199338347 2215 10 -0.44425119246421474 2215 11 -0.598934317014475 2215 12 -0.7780189582570785 2215 13 -0.4442200128477367 2215 14 -0.5728885116675997 2215 15 -0.5910654287881982 2215 16 -0.04328068088729964 2215 17 -0.5902643974052353 2215 18 -1.6118928103591799 2215 19 -0.20127760169629028 2215 20 -4.3797457973544915 2215 21 -2.8757088797615005 2215 22 -1.6613827803051073 2215 23 -1.5500739569166129 2215 24 -1.155100522753603 2215 25 -1.185345674249568 2216 1 -0.034689120034002485 2216 2 -0.7903475125226969 2216 3 -0.0057632721070426385 2216 4 -0.1953067029716813 2216 5 -0.0835675206353983 2216 6 -0.9545920911254768 2216 7 -9.157555537684065 2216 8 -10.73130440183072 2216 9 -5.58307906065901 2216 10 -3.2680556522133735 2216 11 -2.0646084278498726 2216 12 -1.8864742510441666 2216 13 -1.6177991219107177 2216 14 -1.6531707486318652 2216 15 -2.5032546220833822 2216 16 -1.5375836129782456 2216 17 -1.87952352537404 2216 18 -1.217048023809319 2216 19 -0.897450860081527 2216 20 -1.8385327860953993 2216 21 -4.2611682744374875 2216 22 -2.769652390878864 2216 23 -2.7185721254147843 2216 24 -3.1310124756240825 2216 25 -2.704783225945185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24781.0 3 3.0 4 202.0 5 1141.0 6 1374.0 7 1096.0 8 909.0 9 810.0 10 805.0 11 771.0 12 784.0 13 721.0 14 732.0 15 821.0 16 794.0 17 946.0 18 1068.0 19 1324.0 20 1723.0 21 2196.0 22 2866.0 23 3224.0 24 3839.0 25 4510.0 26 4916.0 27 5498.0 28 6077.0 29 7119.0 30 8561.0 31 10594.0 32 13326.0 33 17699.0 34 23449.0 35 32701.0 36 46428.0 37 72120.0 38 86450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.528750015961585 17.422905520156295 12.260544226373653 25.787800237508456 2 14.805996810207336 19.342647527910685 41.05518341307815 24.796172248803828 3 19.85663210491529 23.136383239889856 28.33672315807472 28.670261497120137 4 12.085173887761036 16.064342042173777 37.09945625006376 34.751027820001426 5 13.356037798856448 37.33185142510458 35.6476253355324 13.664485440506569 6 33.09380392916591 35.732620947904266 17.573165229405273 13.600409893524548 7 28.742227254256004 30.529735145206267 21.055738861300735 19.672298739237 8 25.429156305665096 36.8646352714476 20.354672098259353 17.35153632462796 9 25.996365036031005 15.515892133846204 19.169719169719173 39.31802366040362 10 14.888485503625368 29.196198207201785 33.66238693031746 22.252929358855383 11 35.515683865475104 22.376973546699837 22.909200283446424 19.198142304378635 12 23.32378311140113 24.488672281245538 30.938385774587573 21.249158832765758 13 29.287210885047333 21.31408704163459 25.106418772526368 24.292283300791706 14 20.96775015803748 21.484685658353555 26.775372662574686 30.77219152103428 15 23.938233779739903 30.00958406190896 22.508526246565285 23.543655911785848 16 22.806154442240928 27.908601026759992 26.778604238571308 22.506640292427775 17 22.515332419081023 28.27614158335585 26.6473110276162 22.56121496994693 18 22.09553959915273 27.774591595105 28.077916185553082 22.051952620189184 19 24.192606830307867 26.869004616588622 26.291102551487327 22.647286001616187 20 23.72193560227574 27.15083912804095 27.104957278900464 22.022267990782847 21 23.601981020740563 26.425419870871703 26.544199549860704 23.42839955852703 22 23.310919314630627 27.26362046727876 26.687806196057238 22.73765402203337 23 23.153668701647952 27.095499674997768 26.558927365187802 23.191904258166478 24 23.81059194477935 26.802815070465975 26.793383921675584 22.593209063079094 25 23.34074236453453 26.655689065391496 26.96437037679002 23.03919819328395 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 112.0 2 152.0 3 192.0 4 192.0 5 192.0 6 253.0 7 314.0 8 314.0 9 314.0 10 491.5 11 669.0 12 669.0 13 669.0 14 1077.0 15 1485.0 16 1485.0 17 1485.0 18 2576.5 19 3668.0 20 3668.0 21 3668.0 22 5741.5 23 7815.0 24 7815.0 25 7815.0 26 11299.0 27 14783.0 28 14783.0 29 14783.0 30 19770.0 31 24757.0 32 24757.0 33 24757.0 34 28691.0 35 32625.0 36 32625.0 37 32625.0 38 36462.0 39 40299.0 40 40299.0 41 40299.0 42 43409.5 43 46520.0 44 46520.0 45 46520.0 46 49756.5 47 52993.0 48 52993.0 49 52993.0 50 52204.0 51 51415.0 52 51415.0 53 51415.0 54 46833.5 55 42252.0 56 42252.0 57 42252.0 58 36644.5 59 31037.0 60 31037.0 61 31037.0 62 25857.0 63 20677.0 64 20677.0 65 20677.0 66 16244.0 67 11811.0 68 11811.0 69 11811.0 70 8656.5 71 5502.0 72 5502.0 73 5502.0 74 3873.5 75 2245.0 76 2245.0 77 2245.0 78 1544.0 79 843.0 80 843.0 81 843.0 82 553.5 83 264.0 84 264.0 85 264.0 86 168.0 87 72.0 88 72.0 89 72.0 90 47.0 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20719816095703636 2 0.1281927121296301 3 0.13227041271427042 4 0.07288889795044574 5 0.023191922075141827 6 0.020643359209741627 7 0.28212590919980224 8 0.27702878346900184 9 0.019623934063581545 10 0.03618959268868285 11 0.016310802338561286 12 0.016820514911641323 13 0.015291377192401206 14 0.016820514911641323 15 0.015291377192401206 16 0.019369077777041527 17 0.018349652630881445 18 0.015036520905861186 19 0.02472105979438195 20 0.016820514911641323 21 0.014017095759701106 22 0.016310802338561286 23 0.018604508917421467 24 0.015546233478941225 25 0.016310802338561286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 392378.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.0425470738469 #Duplication Level Percentage of deduplicated Percentage of total 1 94.2459416549831 82.97652754677878 2 4.257168840337043 7.496239760533766 3 0.7371443701334177 1.9470020372307797 4 0.2692207980482615 0.9481153914169076 5 0.14027031659654576 0.6174877976007395 6 0.08204105493645204 0.4333862064738384 7 0.056343309286118054 0.34724259220835546 8 0.03700558287260657 0.26064526176452857 9 0.02728682500410097 0.21621614174274442 >10 0.12615712547197627 2.0012008762621707 >50 0.011288983442757599 0.7128124762648108 >100 0.009841677776542686 1.894292133695174 >500 2.8946111107478487E-4 0.14883177802744268 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 584 0.14883607133937174 No Hit GTACATGGGAAGCAGTGGTATCAAC 468 0.11927274210072938 No Hit GTCCTACAGTGGACATTTCTAAATT 454 0.11570475408916912 No Hit CCCATGTACTCTGCGTTGATACCAC 394 0.10041337689676792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.548562865400201E-4 13 0.0 0.0 0.0 0.0 2.548562865400201E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACA 25 0.005942581 19.044912 4 AGTACTG 45 3.468641E-5 16.922316 5 ACTGGAC 45 6.600931E-4 14.820296 3 GTGCAAT 40 0.005165855 14.2928295 1 GTCCTAT 65 5.281294E-5 13.193382 1 GTGTAAA 60 3.9778493E-4 12.704737 1 CTATACT 60 3.9993972E-4 12.696609 4 TAAGGCT 60 3.9993972E-4 12.696609 4 TCTTGGA 55 0.002996204 12.125696 2 TGTTAGA 55 0.002996204 12.125696 2 TGTAGGG 55 0.002996204 12.125696 2 TAGGACC 95 1.0041149E-6 12.028367 4 GTGTAGG 65 7.8009337E-4 11.72745 1 GTCTTAG 65 7.8009337E-4 11.72745 1 CTACACC 65 7.842956E-4 11.719948 4 ACAGATC 65 7.919095E-4 11.706466 8 CTCTTAG 60 0.005732806 11.116645 1 TACTGTT 60 0.005776685 11.105269 7 CTAGCAC 70 0.0014518545 10.888381 3 ATGCCGG 70 0.0015086589 10.838428 19 >>END_MODULE