FastQCFastQC Report
Thu 26 May 2016
SRR1926885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392378
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10690.27244137031128146No Hit
GGTATCAACGCAGAGTACTTTTTTT6560.1671857239702532No Hit
GTACATGGGAAGCAGTGGTATCAAC5610.14297437674895128No Hit
TATCAACGCAGAGTACTTTTTTTTT5280.13456411929313059No Hit
CTGTAGGACGTGGAATATGGCAAGA4510.11494018522954907No Hit
GTCCTACAGTGGACATTTCTAAATT4400.11213676607760885No Hit
CCCATGTACTCTGCGTTGATACCAC4380.1116270535045288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCA350.00217897516.2715110
TAGGACC901.2914825E-1015.8640134
AACACTT456.6480465E-414.8064125
GCCCTAG400.005189291714.2830881
TCAGAAC400.005202519214.2776113
TATACAG602.5123005E-514.2776115
TACTGGG400.00529590914.2393877
TTGGACT551.9224294E-413.8449564
TCCAGAC500.001474386213.3257713
GTCCTAA801.9373965E-613.0928311
GATATAC1650.012.6960781
GTAGGAC2750.012.4604613
CCCTACA701.0635995E-412.2426472
GCGAGAA701.09709064E-412.20207713
GTATAAT550.003008669712.1189831
CAAGACA550.003017329612.1143374
CACTGTT1650.012.0819037
TGACCAG550.003084435112.07882414
TCTAACA657.8457274E-411.7194582
ACTGAGC1302.6211637E-911.6821118
Produced by FastQC (version 0.11.5)