##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392378 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.922291769671084 31.0 30.0 33.0 26.0 34.0 2 30.25940037412903 31.0 30.0 34.0 26.0 34.0 3 30.340747952229737 31.0 30.0 34.0 26.0 34.0 4 33.96371865904816 35.0 35.0 37.0 30.0 37.0 5 33.98028431767327 37.0 35.0 37.0 30.0 37.0 6 33.95074647406327 37.0 35.0 37.0 30.0 37.0 7 33.9537869095617 37.0 35.0 37.0 30.0 37.0 8 33.98147704509427 37.0 35.0 37.0 30.0 37.0 9 35.54418698296031 39.0 35.0 39.0 30.0 39.0 10 35.42960614509478 39.0 35.0 39.0 30.0 39.0 11 35.46992440962541 39.0 35.0 39.0 30.0 39.0 12 35.35636044834318 39.0 35.0 39.0 30.0 39.0 13 35.37957785604698 39.0 35.0 39.0 30.0 39.0 14 36.49078439667871 40.0 37.0 41.0 30.0 41.0 15 36.47742992726402 40.0 37.0 41.0 30.0 41.0 16 36.44689049844793 40.0 37.0 41.0 30.0 41.0 17 36.417870013099616 40.0 36.0 41.0 30.0 41.0 18 36.3940129161166 40.0 36.0 41.0 30.0 41.0 19 36.46382315012564 40.0 37.0 41.0 30.0 41.0 20 36.424493727986786 40.0 37.0 41.0 30.0 41.0 21 36.393834516716026 40.0 37.0 41.0 30.0 41.0 22 36.302855919546964 40.0 37.0 41.0 30.0 41.0 23 36.23784717797634 40.0 37.0 41.0 30.0 41.0 24 36.15534255233474 40.0 37.0 41.0 29.0 41.0 25 36.04780849079204 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06700508539747219 1101 2 -0.0311161855138522 1101 3 0.2979363801002535 1101 4 0.34796846417867044 1101 5 0.23943883187282466 1101 6 0.10431932840687352 1101 7 -0.10600910479104186 1101 8 -0.29135840501171373 1101 9 -0.38012363280558503 1101 10 -0.38459676872936654 1101 11 -0.07841829886658047 1101 12 0.04904955967720781 1101 13 -0.08524614729002167 1101 14 -0.07798049684188868 1101 15 -0.25211927851208316 1101 16 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25 0.21522018366371043 2210 1 0.03911709781766248 2210 2 0.14914990671770667 2210 3 0.3260079168045955 2210 4 0.20424664559236305 2210 5 0.09740721985526335 2210 6 -0.008135025163724663 2210 7 0.1235795483069495 2210 8 0.03698660331723147 2210 9 0.22227629774130264 2210 10 0.1618364341985199 2210 11 0.3385146951593683 2210 12 -0.07801436431504527 2210 13 0.17423447366419254 2210 14 -0.05382112365621339 2210 15 -0.007400156659855384 2210 16 0.027928846473287194 2210 17 0.05072913900797715 2210 18 0.04580877724261967 2210 19 0.07243629059072276 2210 20 0.2769288535233869 2210 21 0.09934845364733036 2210 22 0.13945887563964732 2210 23 0.03468333308873639 2210 24 0.13178995070194333 2210 25 -0.06810861727345241 2211 1 -0.3282777291359871 2211 2 -0.06876823767102636 2211 3 -0.024627503683767316 2211 4 0.032328969998218327 2211 5 -0.004927581008949744 2211 6 7.160204520317848E-4 2211 7 0.08397404914262552 2211 8 0.06575632326342173 2211 9 0.14650737270767422 2211 10 0.23357729270408356 2211 11 -0.057639660553810756 2211 12 0.11944752064684394 2211 13 0.02928522577376924 2211 14 -0.07132958234114284 2211 15 0.010487923728220494 2211 16 0.22999714780629432 2211 17 0.057335575761470636 2211 18 0.02816018974109369 2211 19 -0.22493489280986978 2211 20 -0.10411706796370623 2211 21 0.008236697094403667 2211 22 -0.27088161190672366 2211 23 -0.36591692780564244 2211 24 -0.22564299128982412 2211 25 -0.37806591620133645 2212 1 0.20671074453544946 2212 2 0.3273532538763355 2212 3 0.24366801520323378 2212 4 0.16525079318029867 2212 5 0.24860109326105118 2212 6 0.16138697410405456 2212 7 0.1767559386516453 2212 8 0.21214069598848795 2212 9 0.3268185835788202 2212 10 0.23476025800376732 2212 11 0.11958775188653448 2212 12 0.06675501073824108 2212 13 -0.03956942115658535 2212 14 -0.025547814161896554 2212 15 0.10590873582884797 2212 16 0.0971865435840158 2212 17 -0.15608855268632027 2212 18 -0.012530783323697392 2212 19 -0.005545383791023539 2212 20 -0.1761111228047696 2212 21 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0.2740977819989183 2214 8 0.6465255540326567 2214 9 0.42707535124076657 2214 10 0.4349338818513715 2214 11 0.32586510986956796 2214 12 0.4353315397285371 2214 13 0.28715642434264765 2214 14 0.28162211712218266 2214 15 0.3043534574193387 2214 16 0.4516653052302644 2214 17 0.4091915686058343 2214 18 0.29951528813107586 2214 19 0.15955377743460986 2214 20 0.2919249713922838 2214 21 0.4282352063370922 2214 22 0.4274711012715713 2214 23 0.34897573766841816 2214 24 0.21694049111923874 2214 25 0.3587483354384986 2215 1 0.27739549548885023 2215 2 0.2261330975528928 2215 3 0.1446017885293962 2215 4 0.06878914960356042 2215 5 0.031924008261995596 2215 6 0.13487352960954269 2215 7 0.09553171190996324 2215 8 -0.03335155810252388 2215 9 0.008192058989131112 2215 10 0.21994442020024252 2215 11 -0.24016211122142295 2215 12 0.005931332533876343 2215 13 -0.10107634813618915 2215 14 0.04121915923985853 2215 15 0.16539014516270356 2215 16 0.193522198024894 2215 17 0.2667988275634343 2215 18 -0.20418206477857836 2215 19 -0.1683281930580094 2215 20 0.08768105326989684 2215 21 0.19083299864231407 2215 22 -0.07954136613098939 2215 23 0.11223798180087385 2215 24 0.22052710205768733 2215 25 0.12976966219875408 2216 1 0.1810433069707642 2216 2 0.23983982093703204 2216 3 -1.5344718260280885 2216 4 -1.4082453366475178 2216 5 -0.30483600591737314 2216 6 0.040158720609014154 2216 7 -0.1440152234895038 2216 8 0.2396181756967053 2216 9 0.0590858656433042 2216 10 -0.06960167118916871 2216 11 -0.01168858781702653 2216 12 -0.09676781163992132 2216 13 -0.03625637548211813 2216 14 0.08696450452437432 2216 15 -0.17923604278146144 2216 16 0.11686920432121184 2216 17 0.05481726074315674 2216 18 -0.09890024981934431 2216 19 -0.0875395500573859 2216 20 -0.18643653421174378 2216 21 -0.2702496974634201 2216 22 -0.07736382017464649 2216 23 -0.10133032393332542 2216 24 -0.32902472860013177 2216 25 -0.2978395949035857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8175.0 3 7.0 4 132.0 5 839.0 6 1229.0 7 884.0 8 731.0 9 607.0 10 551.0 11 513.0 12 447.0 13 418.0 14 466.0 15 447.0 16 498.0 17 512.0 18 557.0 19 626.0 20 851.0 21 1133.0 22 1402.0 23 1840.0 24 2070.0 25 2549.0 26 2964.0 27 3290.0 28 4071.0 29 4799.0 30 6087.0 31 8124.0 32 10538.0 33 14007.0 34 19092.0 35 26680.0 36 39223.0 37 68813.0 38 157206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04940400865412 18.488998652896274 10.41300975629669 28.04858758215292 2 14.889942241361718 19.96423250403086 39.35189909586301 25.793926158744412 3 19.277375611745516 23.617200244698207 28.652885399673732 28.452538743882545 4 12.55269153724215 16.493279439723942 36.21049090417913 34.74353811885478 5 13.521462760670211 37.87727409431279 34.75738746132319 13.843875683693806 6 33.57588277856326 36.43304024565162 16.76250182167758 13.22857515410753 7 29.397369849884143 31.151652956815884 20.119705223338443 19.331271969961534 8 26.02219492177743 37.644962813055955 18.538927772198747 17.79391449296787 9 26.256053423051434 15.700158026201763 18.791609318448284 39.252179232298516 10 15.179172188806197 29.67020083596697 32.819859312875934 22.330767662350905 11 35.76629685417256 22.56824709897263 22.481082472518928 19.18437357433588 12 23.514495062121696 24.612169480726344 30.6349792927684 21.23835616438356 13 29.598621719639223 21.345760284219352 25.020070188315707 24.035547807825715 14 21.360324381908217 21.60039961567574 26.423312273655185 30.61596372876086 15 23.84686746558061 29.952901117833857 22.509850496730095 23.690380919855443 16 22.987644504251026 28.104420249964317 26.411401308948562 22.496533936836098 17 22.401357874294686 28.221765746295663 26.711742248546045 22.665134130863606 18 22.147435489009236 27.68983752787512 28.059127110544757 22.103599872570882 19 23.72698636002202 26.912451322201154 26.625226823251168 22.73533549452566 20 23.65988485446246 27.071944297900213 27.25595288156932 22.012217966068 21 23.35409014556957 26.28893176760867 26.814776675222586 23.542201411599176 22 23.32342791899648 27.136842534494797 26.643185907466783 22.896543639041944 23 23.132854372198167 26.74927429487457 26.815791955185958 23.302079377741304 24 23.165671877628206 26.64879274577066 27.41030332995224 22.775232046648895 25 23.188420572395636 26.65495946231818 27.064541698632095 23.09207826665409 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 129.0 1 129.0 2 130.5 3 132.0 4 132.0 5 132.0 6 213.0 7 294.0 8 294.0 9 294.0 10 443.0 11 592.0 12 592.0 13 592.0 14 960.5 15 1329.0 16 1329.0 17 1329.0 18 2363.5 19 3398.0 20 3398.0 21 3398.0 22 5438.5 23 7479.0 24 7479.0 25 7479.0 26 10980.5 27 14482.0 28 14482.0 29 14482.0 30 19426.0 31 24370.0 32 24370.0 33 24370.0 34 28253.0 35 32136.0 36 32136.0 37 32136.0 38 36146.0 39 40156.0 40 40156.0 41 40156.0 42 43208.0 43 46260.0 44 46260.0 45 46260.0 46 49734.5 47 53209.0 48 53209.0 49 53209.0 50 52650.0 51 52091.0 52 52091.0 53 52091.0 54 47301.0 55 42511.0 56 42511.0 57 42511.0 58 37221.0 59 31931.0 60 31931.0 61 31931.0 62 26460.5 63 20990.0 64 20990.0 65 20990.0 66 16416.0 67 11842.0 68 11842.0 69 11842.0 70 8717.0 71 5592.0 72 5592.0 73 5592.0 74 3921.0 75 2250.0 76 2250.0 77 2250.0 78 1543.0 79 836.0 80 836.0 81 836.0 82 558.0 83 280.0 84 280.0 85 280.0 86 174.0 87 68.0 88 68.0 89 68.0 90 43.5 91 19.0 92 19.0 93 19.0 94 10.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10856877806604856 2 0.10245222718908809 3 0.014781664619321165 4 0.0 5 0.006116550876960482 6 0.3198446396077252 7 0.020643359209741627 8 0.009174826315440723 9 0.009684538888520763 10 0.004587413157720361 11 0.004332556871180342 12 7.645688596200602E-4 13 0.0012742814327001003 14 2.548562865400201E-4 15 0.003058275438480241 16 5.097125730800402E-4 17 0.0 18 7.645688596200602E-4 19 5.097125730800402E-4 20 0.0012742814327001003 21 0.014526808332781143 22 0.0 23 2.548562865400201E-4 24 0.0 25 0.006881119736580542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 392378.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.1226220617303 #Duplication Level Percentage of deduplicated Percentage of total 1 92.5708359955721 78.79872286389504 2 5.4946515212000016 9.354382896000384 3 0.9615722146124172 2.455546446285414 4 0.36671665909805884 1.2486353430457782 5 0.1768095118251648 0.752524462600627 6 0.10022910015365753 0.5119058287180264 7 0.06091876619524331 0.3629895577913225 8 0.049708232457785335 0.3385036067888557 9 0.030955291840869703 0.23714960473628235 >10 0.16215758671506147 2.3793184190800765 >50 0.012572883019283713 0.774647565465515 >100 0.011674819886175452 2.068614773746931 >500 8.980630681673423E-4 0.44465789359305175 >1k 2.993543560557807E-4 0.2724007382527062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1069 0.27244137031128146 No Hit GGTATCAACGCAGAGTACTTTTTTT 656 0.1671857239702532 No Hit GTACATGGGAAGCAGTGGTATCAAC 561 0.14297437674895128 No Hit TATCAACGCAGAGTACTTTTTTTTT 528 0.13456411929313059 No Hit CTGTAGGACGTGGAATATGGCAAGA 451 0.11494018522954907 No Hit GTCCTACAGTGGACATTTCTAAATT 440 0.11213676607760885 No Hit CCCATGTACTCTGCGTTGATACCAC 438 0.1116270535045288 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.548562865400201E-4 2 0.0 0.0 0.0 0.0 2.548562865400201E-4 3 0.0 0.0 0.0 0.0 2.548562865400201E-4 4 0.0 0.0 0.0 0.0 2.548562865400201E-4 5 0.0 0.0 0.0 0.0 2.548562865400201E-4 6 0.0 0.0 0.0 0.0 2.548562865400201E-4 7 0.0 0.0 0.0 0.0 2.548562865400201E-4 8 0.0 0.0 0.0 0.0 2.548562865400201E-4 9 0.0 0.0 0.0 0.0 2.548562865400201E-4 10 0.0 0.0 0.0 0.0 2.548562865400201E-4 11 0.0 0.0 0.0 0.0 2.548562865400201E-4 12 0.0 0.0 0.0 0.0 2.548562865400201E-4 13 0.0 0.0 0.0 0.0 2.548562865400201E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCCA 35 0.002178975 16.27151 10 TAGGACC 90 1.2914825E-10 15.864013 4 AACACTT 45 6.6480465E-4 14.806412 5 GCCCTAG 40 0.0051892917 14.283088 1 TCAGAAC 40 0.0052025192 14.277611 3 TATACAG 60 2.5123005E-5 14.277611 5 TACTGGG 40 0.005295909 14.239387 7 TTGGACT 55 1.9224294E-4 13.844956 4 TCCAGAC 50 0.0014743862 13.325771 3 GTCCTAA 80 1.9373965E-6 13.092831 1 GATATAC 165 0.0 12.696078 1 GTAGGAC 275 0.0 12.460461 3 CCCTACA 70 1.0635995E-4 12.242647 2 GCGAGAA 70 1.09709064E-4 12.202077 13 GTATAAT 55 0.0030086697 12.118983 1 CAAGACA 55 0.0030173296 12.114337 4 CACTGTT 165 0.0 12.081903 7 TGACCAG 55 0.0030844351 12.078824 14 TCTAACA 65 7.8457274E-4 11.719458 2 ACTGAGC 130 2.6211637E-9 11.682111 8 >>END_MODULE