FastQCFastQC Report
Thu 26 May 2016
SRR1926884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545412
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8540.15657887981929255No Hit
GTCCTACAGTGTGCATTTCTCATTT6930.1270599106730325No Hit
GTATCAACGCAGAGTACTTTTTTTT6820.12504308669409547No Hit
CTGTAGGACGTGGAATATGGCAAGA6580.12064274346732379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC350.002105987416.3563331
GTATGGG456.534348E-414.8418571
CTTATAC400.005152310714.2999163
AGGACTA400.005206134614.2775425
CTAGTAC706.984803E-613.6189693
GCCGGTT707.3874744E-613.545311
GTCTATG500.001449311913.3576711
GTTGTAG500.001449311913.3576711
CCGGTTT655.539085E-513.1285212
TCTACAC751.4223175E-512.7110373
CCAACGA751.5025071E-512.64343719
TCCAACG1155.4369593E-912.36858118
TGGCGAG3850.012.31503718
GATTAAG701.0452849E-412.267251
CGGTTTC701.107206E-412.19188713
CGTTTCC701.107206E-412.19188714
CTTAGAC550.00298481112.1332643
GGACTAA550.003020037712.1142786
ATCCCGT550.003108599712.06762910
CCTACAC959.927917E-712.0420363
Produced by FastQC (version 0.11.5)