FastQCFastQC Report
Thu 26 May 2016
SRR1926884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545412
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11190.20516600294822998No Hit
GTCCTACAGTGGACATTTCTAAATT8860.16244600412165483No Hit
GGTATCAACGCAGAGTACTTTTTTT8240.15107845078582796No Hit
CTGTAGGACGTGGAATATGGCAAGA7950.14576136938681217No Hit
GTCCTACAGTGTGCATTTCTCATTT6580.12064274346732379No Hit
TATCAACGCAGAGTACTTTTTTTTT6420.11770918131614265No Hit
CTTTAGGACGTGAAATATGGCGAGG6040.11074197120708748No Hit
GTCCTAAAGTGTGTATTTCTCATTT5920.10854179959370164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1850.016.9901224
CCCGTTT508.811723E-515.17674812
CCCTAAT456.555882E-414.8354231
CGTTTCC500.00151474413.279654514
TCCTAGG655.2491465E-513.2039392
ATAGGAC655.303904E-513.1893513
TCCAACG655.4995653E-513.13854318
CCAACGA655.4995653E-513.13854319
GTCCTAG956.987102E-813.050711
AGGACCT3400.012.8864475
TTATACT751.4354517E-512.6996874
ATACACT2250.012.6996874
TCCCGTT604.1375458E-412.6472911
GGACCTG3300.012.4110576
TGTAGGA5000.012.3970332
TCCTAGA1001.3663339E-712.3970322
GTAGGAC4850.012.3735143
TAACCTC550.003004859712.1224295
TAATGTG550.003004859712.1224295
CTAAATT2700.011.94904519
Produced by FastQC (version 0.11.5)