##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926884_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 545412 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.009389232360125 31.0 30.0 33.0 26.0 34.0 2 30.278167697080374 31.0 30.0 34.0 26.0 34.0 3 30.321171884740345 31.0 30.0 34.0 26.0 34.0 4 33.932339222459355 35.0 35.0 37.0 30.0 37.0 5 34.001761970767056 37.0 35.0 37.0 30.0 37.0 6 33.97032335188811 37.0 35.0 37.0 30.0 37.0 7 33.98840509559746 37.0 35.0 37.0 30.0 37.0 8 34.03385697417732 37.0 35.0 37.0 30.0 37.0 9 35.59007319237567 39.0 35.0 39.0 30.0 39.0 10 35.477109047839065 39.0 35.0 39.0 30.0 39.0 11 35.526898931449985 39.0 35.0 39.0 30.0 39.0 12 35.41840480224124 39.0 35.0 39.0 30.0 39.0 13 35.433659325427385 39.0 35.0 39.0 30.0 39.0 14 36.55191304921784 40.0 37.0 41.0 30.0 41.0 15 36.54189493447156 40.0 37.0 41.0 30.0 41.0 16 36.51772788277486 40.0 37.0 41.0 30.0 41.0 17 36.4921160517187 40.0 37.0 41.0 30.0 41.0 18 36.47888018598784 40.0 37.0 41.0 30.0 41.0 19 36.53601497583478 40.0 37.0 41.0 30.0 41.0 20 36.495157788974204 40.0 37.0 41.0 30.0 41.0 21 36.45870827924578 40.0 37.0 41.0 30.0 41.0 22 36.37098743702009 40.0 37.0 41.0 30.0 41.0 23 36.303572345309604 40.0 37.0 41.0 30.0 41.0 24 36.23969952989667 40.0 37.0 41.0 30.0 41.0 25 36.11979201044348 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1222919177178099 1101 2 -0.13969409701234525 1101 3 0.2501599033142661 1101 4 0.012211735711076699 1101 5 0.15001260279807127 1101 6 0.04975772438765347 1101 7 0.04559743340073652 1101 8 0.011247119608128742 1101 9 0.046793288332352745 1101 10 0.062439409417443414 1101 11 0.01926440889447889 1101 12 0.25712533752704303 1101 13 0.3925763770424737 1101 14 0.15757329920934637 1101 15 0.10282939619309417 1101 16 0.06484160003122241 1101 17 0.2761104459198904 1101 18 0.10928339766790174 1101 19 0.04489099979225131 1101 20 0.1602688962338661 1101 21 0.15335788060649946 1101 22 -0.21982944377639768 1101 23 -0.09921813827598669 1101 24 0.028659478461115384 1101 25 0.09773278028990262 1102 1 -0.9057156226468912 1102 2 -0.6017257730225154 1102 3 -0.5396602725274136 1102 4 -0.746073348380456 1102 5 -0.7646582301523281 1102 6 -0.6460085961098514 1102 7 -0.6761867163404034 1102 8 -0.541765777490788 1102 9 -0.5702272061074964 1102 10 -0.4954382366322463 1102 11 -0.5771176638316788 1102 12 -0.5358409952242411 1102 13 -0.5796044886867762 1102 14 -0.48885013000978006 1102 15 -0.6604889262650175 1102 16 -0.6999992540569409 1102 17 -0.518853252269615 1102 18 -0.36656134699404674 1102 19 -0.7014190820017063 1102 20 -0.7021150758275212 1102 21 -0.8817828846417939 1102 22 -0.9594949156999348 1102 23 -1.0508892469363431 1102 24 -0.9945614264802174 1102 25 -0.6900073171276162 1103 1 -0.6992965626267456 1103 2 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13 0.10897204099911306 1104 14 0.17652843320513512 1104 15 0.17244698125782776 1104 16 0.33759077196738474 1104 17 0.3491005091538568 1104 18 0.23346286168476382 1104 19 0.399543212981051 1104 20 0.417224632457895 1104 21 0.38178305374712096 1104 22 0.5873972951576576 1104 23 0.7003151348345256 1104 24 0.3137693851157408 1104 25 0.4078502147975769 1105 1 -0.1316367857001488 1105 2 -0.10539653911381208 1105 3 -0.008008971156311162 1105 4 -0.07108104612185429 1105 5 -0.22507976450840772 1105 6 -0.3293627586257699 1105 7 -0.30200101663080403 1105 8 -0.2975300273685306 1105 9 -0.37053571950760755 1105 10 -0.07144362203249699 1105 11 -0.08772847206433454 1105 12 -0.41514644764011877 1105 13 -0.2646823415301114 1105 14 -0.3107334503626049 1105 15 -0.4741733973547113 1105 16 -0.4213078097191598 1105 17 -0.2893126414010112 1105 18 -0.25617573543113537 1105 19 -0.3412674214026694 1105 20 -0.4428400521305562 1105 21 -0.3710090654149454 1105 22 -0.254487459456314 1105 23 -0.6866381617057371 1105 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9 -0.10118902233652705 2211 10 -0.25081766702371056 2211 11 -0.2750177416065753 2211 12 -0.3143491986241145 2211 13 -0.3539091440699096 2211 14 -0.2987830954555051 2211 15 -0.36120371460619083 2211 16 -0.4411384711229758 2211 17 -0.3228605112600249 2211 18 -0.3748203003661459 2211 19 -0.5701832018491189 2211 20 -0.6095444302958768 2211 21 -0.8866160389694073 2211 22 -0.7762609614019453 2211 23 -0.7002911209410101 2211 24 -0.5908356385464373 2211 25 -0.3184470045465986 2212 1 -0.24362266380178355 2212 2 -0.2436143422385868 2212 3 -0.029890154336399632 2212 4 -0.23149871733878769 2212 5 -0.292106129449742 2212 6 -0.11039019878229084 2212 7 -0.2882449131219431 2212 8 -0.32316892133790276 2212 9 -0.3445338018557891 2212 10 -0.42157026464538205 2212 11 -0.06772060193259932 2212 12 -0.19478275887612284 2212 13 -0.20443703728301443 2212 14 -0.3546205195144623 2212 15 -0.5076630248536205 2212 16 -0.3191568251213397 2212 17 -0.43278181764208057 2212 18 -0.5353354048856005 2212 19 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0.5768494783539424 2214 7 0.5385557220531609 2214 8 0.6415641478464664 2214 9 0.5245298221936068 2214 10 0.4079699436636446 2214 11 0.5527242235718148 2214 12 0.3082155517204441 2214 13 0.6183125809523986 2214 14 0.42414373007079575 2214 15 0.281181839207747 2214 16 0.44252314200377896 2214 17 0.41341144402267105 2214 18 0.5376564626408182 2214 19 0.6323858443026822 2214 20 0.4595924960029052 2214 21 0.5989214099859197 2214 22 0.645146222312853 2214 23 0.5377886532222718 2214 24 0.6553332940733156 2214 25 0.67146040546519 2215 1 0.603403834531612 2215 2 0.6565812744042709 2215 3 0.37547900772941745 2215 4 0.6419669146187985 2215 5 0.6013831824172371 2215 6 0.5492136775159224 2215 7 0.3037476740368561 2215 8 0.3275266760006801 2215 9 0.1945020140071776 2215 10 0.25331616187612127 2215 11 0.21652324965614866 2215 12 0.364700143971902 2215 13 0.3471903130123408 2215 14 0.4661315749992667 2215 15 0.5131662867559683 2215 16 0.3071098569887454 2215 17 0.6115781169807804 2215 18 0.37335537925400786 2215 19 0.4162029897483066 2215 20 0.19561101801667036 2215 21 0.4435441120356671 2215 22 0.4970569341269311 2215 23 0.6433012172377204 2215 24 0.6138865134056672 2215 25 0.3369669296935385 2216 1 -0.11241537450793615 2216 2 0.017297314689212584 2216 3 -1.7641181428478383 2216 4 -1.4172568633227414 2216 5 -0.672075427260971 2216 6 -0.4218069562349953 2216 7 -0.3442442198467148 2216 8 -0.3555671584380278 2216 9 -0.0681422994293257 2216 10 -0.406736650700644 2216 11 -0.2532261976541008 2216 12 -0.2558107870032842 2216 13 -0.47710961329568846 2216 14 -0.6468013653102389 2216 15 -0.5146155850200032 2216 16 -0.2219002142468227 2216 17 -0.4224000210688388 2216 18 -0.42865272686242406 2216 19 -0.538844910030619 2216 20 -0.4867520377435852 2216 21 -0.5570822696887205 2216 22 -0.5218020685751128 2216 23 -0.4864027866925156 2216 24 -0.5945020889033472 2216 25 -0.5911158509500325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11373.0 3 7.0 4 173.0 5 1151.0 6 1513.0 7 1193.0 8 940.0 9 843.0 10 732.0 11 716.0 12 659.0 13 589.0 14 596.0 15 574.0 16 657.0 17 707.0 18 739.0 19 942.0 20 1172.0 21 1594.0 22 1953.0 23 2393.0 24 2929.0 25 3338.0 26 4025.0 27 4584.0 28 5370.0 29 6743.0 30 8657.0 31 10979.0 32 14449.0 33 19238.0 34 26107.0 35 36837.0 36 54058.0 37 94764.0 38 222118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41498171886701 18.876187539462286 10.492746281367928 28.216084460302778 2 14.695944395807295 20.23158625018583 39.77399325687207 25.298476097134813 3 19.29960647103913 23.79841634850602 28.8590960936841 28.042881086770755 4 12.428769444016634 16.789692929381825 37.063540956194586 33.71799667040696 5 13.15999853313286 38.40386519490997 35.09442957204151 13.341706699915656 6 32.64089987526263 37.47014950086654 17.03069863966354 12.858251984207293 7 28.73809838182005 31.424123057964 20.80674266097443 19.031035899241523 8 25.411477357743255 38.317637049098565 18.964632017485954 17.306253575672226 9 26.0031575878376 15.860736073699053 19.204075158658704 38.93203117980464 10 14.889052684848018 29.79955775091035 33.503852260934295 21.807537303307335 11 35.32536350134766 22.697702561469775 23.195144758796456 18.78178917838611 12 23.26231642250784 24.81014282833098 31.102290020351663 20.825250728809518 13 29.35223043215196 21.580645752736473 25.376505747969418 23.69061806714215 14 20.93279942502182 22.064971067743283 26.921849904292532 30.080379602942365 15 23.19118000282363 30.708273056140754 22.90423306325484 23.19631387778077 16 22.507879407930563 28.32681528907664 27.101129610989183 22.064175692003616 17 21.7672145093984 28.736991485335857 27.302662941042733 22.19313106422301 18 21.722047124268208 28.043908333012475 28.796554512301775 21.43749003041754 19 23.48375812002325 27.015223382 26.998722064644827 22.502296433331928 20 23.36012673081436 27.561935285144333 27.686795939920206 21.391142044121096 21 23.05600481162418 26.72468487895758 26.910988620068725 23.308321689349516 22 23.052298079250182 27.711344818229154 26.96016955989234 22.276187542628325 23 22.638340627526766 27.549316020395626 27.133849518986597 22.67849383309101 24 23.00591112773463 27.0129736786136 27.79476799190337 22.1863472017484 25 22.87218340138948 27.244340215958328 27.41724622879701 22.46623015385518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 141.0 1 141.0 2 160.0 3 179.0 4 179.0 5 179.0 6 286.0 7 393.0 8 393.0 9 393.0 10 650.5 11 908.0 12 908.0 13 908.0 14 1462.5 15 2017.0 16 2017.0 17 2017.0 18 3665.0 19 5313.0 20 5313.0 21 5313.0 22 8422.5 23 11532.0 24 11532.0 25 11532.0 26 17021.0 27 22510.0 28 22510.0 29 22510.0 30 29707.5 31 36905.0 32 36905.0 33 36905.0 34 42943.0 35 48981.0 36 48981.0 37 48981.0 38 53934.5 39 58888.0 40 58888.0 41 58888.0 42 63212.5 43 67537.0 44 67537.0 45 67537.0 46 70393.0 47 73249.0 48 73249.0 49 73249.0 50 71421.0 51 69593.0 52 69593.0 53 69593.0 54 62716.5 55 55840.0 56 55840.0 57 55840.0 58 48112.0 59 40384.0 60 40384.0 61 40384.0 62 33183.0 63 25982.0 64 25982.0 65 25982.0 66 20242.0 67 14502.0 68 14502.0 69 14502.0 70 10530.5 71 6559.0 72 6559.0 73 6559.0 74 4592.0 75 2625.0 76 2625.0 77 2625.0 78 1799.5 79 974.0 80 974.0 81 974.0 82 629.0 83 284.0 84 284.0 85 284.0 86 188.0 87 92.0 88 92.0 89 92.0 90 57.0 91 22.0 92 22.0 93 22.0 94 11.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10780840905590637 2 0.1028580229257882 3 0.01650128710039383 4 0.0 5 0.005867124302362251 6 0.342860076419294 7 0.019801544520472596 8 0.01100085806692922 9 0.010817510432480401 10 0.003300257420078766 11 0.004033647957874048 12 3.666952688976407E-4 13 3.666952688976407E-4 14 0.0 15 0.002750214516732305 16 5.500429033464609E-4 17 0.0 18 5.500429033464609E-4 19 1.8334763444882035E-4 20 7.333905377952814E-4 21 0.012100943873622143 22 0.0 23 1.8334763444882035E-4 24 0.0 25 0.00531708139901579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 545412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.18288316679661 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91981112699365 77.38074720817717 2 6.102362434742781 10.274289277708 3 1.0248335144769298 2.5882032004388678 4 0.3525545347815048 1.18716228845743 5 0.17876406634363065 0.7524437255713667 6 0.10475533171852403 0.5291163510695718 7 0.062265321039710796 0.36691719725003397 8 0.03819740636451662 0.2572454237807005 9 0.03344782400224895 0.25341608341484084 >10 0.15709745275000297 2.459995530126788 >50 0.011104722600259541 0.6642771114247978 >100 0.012846611573386141 2.0687138552447655 >500 0.001741913433654946 1.0123610333536501 >1k 2.1773917920686825E-4 0.2051117139820269 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1119 0.20516600294822998 No Hit GTCCTACAGTGGACATTTCTAAATT 886 0.16244600412165483 No Hit GGTATCAACGCAGAGTACTTTTTTT 824 0.15107845078582796 No Hit CTGTAGGACGTGGAATATGGCAAGA 795 0.14576136938681217 No Hit GTCCTACAGTGTGCATTTCTCATTT 658 0.12064274346732379 No Hit TATCAACGCAGAGTACTTTTTTTTT 642 0.11770918131614265 No Hit CTTTAGGACGTGAAATATGGCGAGG 604 0.11074197120708748 No Hit GTCCTAAAGTGTGTATTTCTCATTT 592 0.10854179959370164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 6 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 7 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 8 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 9 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 10 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 11 1.8334763444882035E-4 1.8334763444882035E-4 0.0 0.0 0.0 12 1.8334763444882035E-4 1.8334763444882035E-4 0.0 1.8334763444882035E-4 0.0 13 1.8334763444882035E-4 1.8334763444882035E-4 0.0 1.8334763444882035E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 185 0.0 16.990122 4 CCCGTTT 50 8.811723E-5 15.176748 12 CCCTAAT 45 6.555882E-4 14.835423 1 CGTTTCC 50 0.001514744 13.2796545 14 TCCTAGG 65 5.2491465E-5 13.203939 2 ATAGGAC 65 5.303904E-5 13.189351 3 TCCAACG 65 5.4995653E-5 13.138543 18 CCAACGA 65 5.4995653E-5 13.138543 19 GTCCTAG 95 6.987102E-8 13.05071 1 AGGACCT 340 0.0 12.886447 5 TTATACT 75 1.4354517E-5 12.699687 4 ATACACT 225 0.0 12.699687 4 TCCCGTT 60 4.1375458E-4 12.64729 11 GGACCTG 330 0.0 12.411057 6 TGTAGGA 500 0.0 12.397033 2 TCCTAGA 100 1.3663339E-7 12.397032 2 GTAGGAC 485 0.0 12.373514 3 TAACCTC 55 0.0030048597 12.122429 5 TAATGTG 55 0.0030048597 12.122429 5 CTAAATT 270 0.0 11.949045 19 >>END_MODULE