FastQCFastQC Report
Thu 26 May 2016
SRR1926883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580343
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10470.18041055031248762No Hit
GTCCTACAGTGTGCATTTCTCATTT7690.13250784449885672No Hit
GTCCTAAAGTGTGTATTTCTCATTT7620.13130166125894513No Hit
GTATCAACGCAGAGTACTTTTTTTT7490.12906160667053793No Hit
CTGTAGGACGTGGAATATGGCAAGA7370.12699386397354667No Hit
GTACATGGGAAGCAGTGGTATCAAC6890.11872289318558163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC350.002113241516.3481751
ATCTATG400.00514152914.3046531
GTACTAG400.00514152914.3046531
ACCGTCT400.00523988514.2638678
GTCTTAG1009.496944E-913.3510091
GGTGAGC604.0517526E-412.6789938
TAGACAA701.070972E-412.2356865
ACTGATC701.0787806E-412.2261728
AGTACCT550.00302452612.1120935
GTCTTAA959.891919E-712.0460231
GTCCTAC6100.011.881461
TATAGGA1052.5973895E-711.7998582
TGAGTTA1900.011.48044212
ATAGGAC752.0085038E-411.4367863
TGTAGGA6500.011.4367852
TAGGACC2250.011.4219494
TAGTACT752.032878E-411.4219494
GTATTAA855.1061434E-511.2193361
GTATAAG855.1061434E-511.2193361
CTGTAGG7000.011.1712521
Produced by FastQC (version 0.11.5)